LOCUS AK312769 1658 bp mRNA linear HTC 24-MAY-2008
DEFINITION Homo sapiens cDNA, FLJ93184, Homo sapiens preferentially expressed
antigen in melanoma (PRAME),mRNA.
ACCESSION AK312769
VERSION AK312769.1
KEYWORDS HTC; HTC_FLI; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1658)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Kaida,T., Tsuchiya,K.,
Iida,Y., Takayama,Y., Murakawa,K., Kanehori,K., Andoh,T.,
Kagawa,N., Sato,R., Kawamura,Y., Tanaka,S., Kisu,Y., Sugano,S.,
Goshima,N., Nomura,N. and Isogai,T.
TITLE NEDO functional analysis of protein and research application
project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC and Biological
Information Research Center (BIRC), AIST; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1658
/clone="TESTI2021281"
/clone_lib="TESTI2"
/db_xref="H-InvDB:HIT000431635"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="testis"
CDS 129..1658
/note="Homo sapiens preferentially expressed antigen in
melanoma (PRAME),mRNA"
/protein_id="BAG35634.1"
/translation="MERRRLWGSIQSRYISMSVWTSPRRLVELAGQSLLKDEALAIAA
LELLPRELFPPLFMAAFDGRHSQTLKAMVQAWPFTCLPLGVLMKGQHLHLETFKAVLD
GLDVLLAQEVRPRRWKLQVLDLRKNSHQDFWTVWSGNRASLYSFPEPEAAQPMTKKRK
VDGLSTEAEQPFIPVEVLVDLFLKEGACDELFSYLIEKVKRKKNVLRLCCKKLKIFAM
PMQDIKMILKMVQLDSIEDLEVTCTWKLPTLAKFSPYLGQMINLRRLLLSHIHASSYI
SPEKEEQYIAQFTSQFLSLQCLQALYVDSLFFLRGRLDQLLRHVMNPLETLSITNCRL
SEGDVMHLSQSPSVSQLSVLSLSGVMLTDVSPEPLQALLERASATLQDLVFDECGITD
DQLLALLPSLSHCSQLTTLSFYGNSISISALQSLLQHLIGLSNLTHVLYPVPLESYED
IHGTLHLERLAYLHARLRELLCELGRPSMVWLSANPCPHCGDRTFYDPEPILCPCFMP
N"
BASE COUNT 367 a 456 c 435 g 400 t
ORIGIN
1 gttctttcct tccagcaact tcgcggtgtg gtgaactctc tgaggaaaaa cacgtgcgtg
61 gcaacaagtg actgagacct agaaatccaa gcgttggagg tcctgaggcc agcctaagtc
121 gcttcaaaat ggaacgaagg cgtttgtggg gttccattca gagccgatac atcagcatga
181 gtgtgtggac aagcccacgg agacttgtgg agctggcagg gcagagcctg ctgaaggatg
241 aggccctggc cattgccgcc ctggagttgc tgcccaggga gctcttcccg ccactcttca
301 tggcagcctt tgacgggaga cacagccaga ccctgaaggc aatggtgcag gcctggccct
361 tcacctgcct ccctctggga gtgctgatga agggacaaca tcttcacctg gagaccttca
421 aagctgtgct tgatggactt gatgtgctcc ttgcccagga ggttcgcccc aggaggtgga
481 aacttcaagt gctggattta cggaagaact ctcatcagga cttctggact gtatggtctg
541 gaaacagggc cagtctgtac tcatttccag agccagaagc agctcagccc atgacaaaga
601 agcgaaaagt agatggtttg agcacagagg cagagcagcc cttcattcca gtagaggtgc
661 tcgtagacct gttcctcaag gaaggtgcct gtgatgaatt gttctcctac ctcattgaga
721 aagtgaagcg aaagaaaaat gtactacgcc tgtgctgtaa gaagctgaag atttttgcaa
781 tgcccatgca ggatatcaag atgatcctga aaatggtgca gctggactct attgaagatt
841 tggaagtgac ttgtacctgg aagctaccca ccttggcgaa attttctcct tacctgggcc
901 agatgattaa tctgcgtaga ctcctcctct cccacatcca tgcatcttcc tacatttccc
961 cggagaagga agagcagtat atcgcccagt tcacctctca gttcctcagt ctgcagtgcc
1021 tgcaggctct ctatgtggac tctttatttt tccttagagg ccgcctggat cagttgctca
1081 ggcacgtgat gaaccccttg gaaaccctct caataactaa ctgccggctt tcggaagggg
1141 atgtgatgca tctgtcccag agtcccagcg tcagtcagct aagtgtcctg agtctaagtg
1201 gggtcatgct gaccgatgta agtcccgagc ccctccaagc tctgctggag agagcctctg
1261 ccaccctcca ggacctggtc tttgatgagt gtgggatcac ggatgatcag ctccttgccc
1321 tcctgccttc cctgagccac tgctcccagc ttacaacctt aagcttctac gggaattcca
1381 tctccatatc tgccttgcag agtctcctgc agcacctcat cgggctgagc aatctgaccc
1441 acgtgctgta tcctgtcccc ctggagagtt atgaggacat ccatggtacc ctccacctgg
1501 agaggcttgc ctatctgcat gccaggctca gggagttgct gtgtgagttg gggcggccca
1561 gcatggtctg gcttagtgcc aacccctgtc ctcactgtgg ggacagaacc ttctatgacc
1621 cggagcccat cctgtgcccc tgtttcatgc ctaactag
//