LOCUS AK312714 3157 bp mRNA linear HTC 24-MAY-2008
DEFINITION Homo sapiens cDNA, FLJ93116, Homo sapiens mitogen-activated
protein kinase kinase kinase 13 (MAP3K13), mRNA.
ACCESSION AK312714
VERSION AK312714.1
KEYWORDS HTC; HTC_FLI; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3157)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Kaida,T., Tsuchiya,K.,
Iida,Y., Takayama,Y., Murakawa,K., Kanehori,K., Andoh,T.,
Kagawa,N., Sato,R., Kawamura,Y., Tanaka,S., Kisu,Y., Sugano,S.,
Goshima,N., Nomura,N. and Isogai,T.
TITLE NEDO functional analysis of protein and research application
project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC and Biological
Information Research Center (BIRC), AIST; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
The reason of remaining 5'-end "N": This sequence was generated
from 5'-end region of cDNA insert including about 20 base of the
sequence before "N". This cDNA was obtained from oligo-capped cDNA
library by which method 5'-end of mRNA was efficiently cloned.
Therefore remaining 5'-end "N" is important for a detection of
transcription start site of this mRNA.
FEATURES Location/Qualifiers
source 1..3157
/clone="OCBBF1000261"
/clone_lib="OCBBF1"
/db_xref="H-InvDB:HIT000431580"
/db_xref="taxon:9606"
/dev_stage="fetal"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="brain"
CDS 257..3157
/note="Homo sapiens mitogen-activated protein kinase
kinase kinase 13 (MAP3K13), mRNA"
/protein_id="BAG35589.1"
/translation="MANFQEHLSCSSSPHLPFSESKTFNGLQDELTAMGNHPSPKLLE
DQQEKGMVRTELIESVHSPVTTTVLTSVSEDSRDQFENSVLQLREHDESETAVSQGNS
NTVDGESTSGTEDIKIQFSRSGSGSGGFLEGLFGCLRPVWNIIGKAYSTDYKLQQQDT
WEVPFEEISELQWLGSGAQGAVFLGKFRAEEVAIKKVREQNETDIKHLRKLKHPNIIA
FKGVCTQAPCYCIIMEYCAHGQLYEVLRAGRKITPRLLVDWSTGIASGMNYLHLHKII
HRDLKSPNVLVTHTDAVKISDFGTSKELSDKSTKMSFAGTVAWMAPEVIRNEPVSEKV
DIWSFGVVLWELLTGEIPYKDVDSSAIIWGVGSNSLHLPVPSTCPDGFKILMKQTWQS
KPRNRPSFRQTLMHLDIASADVLATPQETYFKSQAEWREEVKKHFEKIKSEGTCIHRL
DEELIRRRREELRHALDIREHYERKLERANNLYMELSAIMLQLEMREKELIKREQAVE
KKYPGTYKRHPVRPIIHPNAMEKLMKRKGVPHKSGMQTKRPDLLRSEGIPTTEVAPTA
SPLSGSPKMSTSSSKSRYRSKPRHRRGNSRGSHSDFAAILKNQPAQENSPHPTYLHQA
QSQYPSLHHHNSLQQQYQQPPPAMSQSHHPRLNMHGQDIATCANNLRYFGPAAALRSP
LSNHAQRQLPGSSPDLISTAMAADCWRSSEPDKGQAGPWGCCQADAYDPCLQCRPEQY
GSLDIPSAEPVGRSPDLSKSPAHNPLLENAQSSEKTEENEFSGCRSESSLGTSHLGTP
PALPRKTRPLQKSGDDSSEEEEGEVDSEVEFPRRQRPHRCISSCQSYSTFSSENFSVS
DGEEGNTSDHSNSPDELADKLEDRLAEKLDDLLSQTPEIPIDISSHSDGLSDKECAVR
RVKTQMSLGKLCVEERGYENPMQFEESDCDSSDGECSDATVRTNKHYSSATW"
BASE COUNT 897 a 778 c 796 g 685 t
ORIGIN
1 ntgggtgggg cggagcgtct gggatgcgct gggagcctag gatccccgac agaacactga
61 aatctatgga ctctaaaatg gacttcattt aaagaaaccc acggaccatt aatggacaaa
121 aacatgagtc aatatgtatt gtggcattca aatcctagca ctctgggaga ggttttggag
181 ccctctctta agtcagaact ctgtcccaaa aatcttctga gtgtcatctc aggactttgg
241 ttatactcat ggcacgatgg ccaactttca ggagcacctg agctgctcct cttctccaca
301 cttacccttc agtgaaagca aaaccttcaa tggactacaa gatgagctca cagctatggg
361 gaaccaccct tctcccaagc tgctcgagga ccagcaggaa aaggggatgg tacgaacaga
421 gctaatcgag agcgtgcaca gccccgtcac cacaacagtg ttgacgagcg taagtgagga
481 ttccagggac cagtttgaga acagcgttct tcagctaagg gaacacgatg aatcagagac
541 ggcggtgtct caggggaaca gcaacacggt ggacggagag agcacaagcg gaactgaaga
601 cataaagatt cagttcagca ggtcaggcag tggcagtggt gggtttcttg aaggactatt
661 tggatgctta aggcctgtat ggaatatcat tgggaaggca tattccactg attacaaatt
721 gcagcagcaa gatacttggg aagtgccatt tgaggagatc tcagagctgc agtggctggg
781 tagtggagcc caaggagcgg tcttcttggg caagttccgg gcggaagagg tggccatcaa
841 gaaagtgaga gaacagaatg agacggatat caagcatttg aggaagttga agcaccctaa
901 catcatcgca ttcaagggtg tttgtactca ggccccatgt tattgtatta tcatggaata
961 ctgtgcccat ggacaactct acgaggtctt acgagctggc aggaagatca cacctcgatt
1021 gctagtagac tggtccacag gaattgcaag tggaatgaat tatttgcacc tccataaaat
1081 tattcatcgt gatctcaaat cacctaatgt tttagtgacc cacacagatg cggtaaaaat
1141 ttcagatttt ggtacatcta aggaactcag tgacaaaagt accaagatgt catttgctgg
1201 cacggtcgca tggatggcgc cagaggtgat acggaatgaa cctgtctctg aaaaagttga
1261 tatatggtct tttggagtgg tgctttggga gctgctgaca ggagagatcc cttacaaaga
1321 tgtagattct tcagccatta tctggggtgt tggaagcaac agcctccacc ttccagttcc
1381 ttccacttgc cctgatggat tcaaaatcct tatgaaacag acgtggcaga gtaaacctcg
1441 aaaccgacct tcttttcggc agacactcat gcatttagac attgcctctg cagatgtact
1501 tgccacccca caagaaactt acttcaagtc tcaggctgaa tggagagaag aagtgaaaaa
1561 acattttgag aagatcaaaa gtgaaggaac ttgtatacac cggttagatg aagaactgat
1621 tcgaaggcgc agagaagagc tcaggcatgc gctggatatt cgtgaacact atgagcggaa
1681 gcttgagcgg gcgaataatt tatacatgga attgagtgcc atcatgctgc agctagaaat
1741 gcgggagaag gagctcatta agcgtgagca agcagtggaa aagaagtatc ctgggaccta
1801 caaacgacac cctgttcgtc ctatcatcca tcccaatgcc atggagaaac tcatgaaaag
1861 gaaaggagtg cctcacaaat ctgggatgca gaccaaacgg ccagacttgt tgagatcaga
1921 agggatcccc accacagaag tggctcccac tgcatcccct ttgtccggaa gtcccaaaat
1981 gtccacttct agcagcaaga gccgatatcg aagcaaacca cgccaccgcc gagggaatag
2041 cagaggcagc catagtgact ttgccgcaat cttgaaaaac cagccagccc aggaaaattc
2101 accccatccc acttacctgc accaagctca atcccaatac ccttctcttc atcaccataa
2161 ttctctgcag cagcaatacc agcagccccc tcctgccatg tcccagagtc accatcccag
2221 actcaatatg cacggacagg acatagcaac ctgcgccaac aacctgaggt atttcggccc
2281 agcagcagcc ctgcggagcc cactcagcaa ccatgctcag agacagctgc ccggctcgag
2341 ccctgacctc atctccacag ccatggctgc agactgctgg agaagttctg agcctgacaa
2401 gggccaagct ggtccctggg gctgttgcca ggctgacgct tatgacccct gccttcagtg
2461 caggccagaa cagtatgggt ccttagacat accctctgct gagccagtgg ggaggagccc
2521 tgacctttcc aagtcaccag cacataatcc tctcttggaa aacgcccaga gttctgagaa
2581 aacggaagaa aatgaattca gcggctgtag gtctgagtca tccctcggca cctctcatct
2641 cggcacccct ccagcgctac ctcgaaaaac aaggcctctg cagaagagtg gagatgactc
2701 ctcagaagag gaagaagggg aagtagatag tgaagttgaa tttccacgaa gacagaggcc
2761 ccatcgctgt atcagcagct gccagtcata ttcaaccttt agctctgaga atttctctgt
2821 gtctgatgga gaagagggaa ataccagtga ccactcaaac agtcctgatg agttagctga
2881 taaacttgaa gaccgcttgg cagagaagct agacgacctg ctgtcccaga cgccagagat
2941 tcccattgac atatcctcac actcggatgg gctctctgac aaggagtgtg ccgtgcgccg
3001 tgtgaagact cagatgtctc tgggcaagct gtgtgtggag gaacgtggct atgagaaccc
3061 catgcagttt gaagaatcgg actgtgactc ttcagatggg gagtgttctg atgccacagt
3121 taggaccaat aaacactaca gctctgctac ctggtaa
//