LOCUS AK312709 3333 bp mRNA linear HTC 24-MAY-2008
DEFINITION Homo sapiens cDNA, FLJ93109, highly similar to Homo sapiens BUB1
budding uninhibited by benzimidazoles 1 homologbeta (yeast)
(BUB1B), mRNA.
ACCESSION AK312709
VERSION AK312709.1
KEYWORDS HTC; HTC_FLI; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3333)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Kaida,T., Tsuchiya,K.,
Iida,Y., Takayama,Y., Murakawa,K., Kanehori,K., Andoh,T.,
Kagawa,N., Sato,R., Kawamura,Y., Tanaka,S., Kisu,Y., Sugano,S.,
Goshima,N., Nomura,N. and Isogai,T.
TITLE NEDO functional analysis of protein and research application
project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC and Biological
Information Research Center (BIRC), AIST; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3333
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP8005110"
/clone_lib="NT2RP8"
/db_xref="H-InvDB:HIT000431575"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 181..3333
/note="highly similar to Homo sapiens BUB1 budding
uninhibited by benzimidazoles 1 homologbeta (yeast)
(BUB1B), mRNA"
/protein_id="BAG35587.1"
/translation="MAAVKKEGGALSEAMSLEGDEWELSKENVQPLRQGRIMSTLQGA
LAQESACNNTLQQQKRAFEYEIRFYTGNDPLDVWDRYISWTEQNYPQGGKESNMSTLL
ERAVEALQGEKRYYSDPRFLNLWLKLGRLCNEPLDMYSYLHNQGIGVSLAQFYISWAE
EYEARENFRKADAIFQEGIQQKAEPLERLQSQHRQFQARVSRQTLLALEKEEEEEVFE
SSVPQRSTLAELKSKGKKTARAPIIRVGGALKAPSQNRGLQNPFPQQMQNNSRITVFD
ENADEAPTAELSKPTVQPWIAPPMPRAKENELQAGPWNTGRSLEHRPRGNTASLIAVP
AVLPSFTPYVEETAQQPVMTPCKIEPSINHILSTRKPGKEEGDPLQRVQSHQQASEEK
KEKMMYCKEKIYAGVGEFSFEEIRAEVFRKKLKEQREAELLTSAEKRAEMQKQIEEME
KKLKEIQTTQQERTGDQQEETMPTKETTKLQIASESQKIPGMTLSSSVCQVNCCARET
SLAENIWQEQPHSKGPSVPFSIFDEFLLSEKKNKSPPADPPRVLAQRRPLAVLKTSES
ITSNEDVSPDVCDEFTGIEPLSEDAIITGFRNVTICPNPEDTCDFARAARFVSTPFHE
IMSLKDLPSDPERLLPEEDLDVKTSEDQQTACGTIYSQTLSIKKLSPIIEDSREATHS
SGFSGSSASVASTSSIKCLQIPEKLELTNETSENPTQSPWCSQYRRQLLKSLPELSAS
AELCIEDRPMPKLEIEKEIELGNEDYCIKREYLICEDYKLFWVAPRNSAELTVIKVSS
QPVPWDFYINLKLKERLNEDFDHFCSCYQYQDGCIVWHQYINCFTLQDLLQHSEYITH
EITVLIIYNLLTIVEMLHKAEIVHGDLSPRCLILRNRIHDPYDCNKNNQALKIVDFSY
SVDLRVQLDVFTLSGFRTVQILEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQVF
WDGSFWKLSQNISELKDGELWNKFFVRILNANDEATVSVLGELAAEMNGVFDTTFQSH
LNKALWKVGKLTSPGALLFQ"
BASE COUNT 1037 a 683 c 793 g 820 t
ORIGIN
1 aaaatttgaa acttggcggc taggggtgtg ggcttgaggt ggccggtttg ttagggagtc
61 gtgtgcgtgc cttggtcgct tctgtagctc cgagggcagg ttgcaggaag aaagcccagg
121 cggtctgtgg cccagaggaa aggcctgcag caggacgagg acctgagcca ggaatgcagg
181 atggcggcgg tgaagaagga agggggtgct ctgagtgaag ccatgtccct ggagggagat
241 gaatgggaac tgagtaaaga aaatgtacaa cctttaaggc aagggcggat catgtccacg
301 cttcagggag cactggcaca agaatctgcc tgtaacaata ctcttcagca gcagaaacgg
361 gcatttgaat atgaaattcg attttacact ggaaatgacc ctctggatgt ttgggatagg
421 tatatcagct ggacagagca gaactatcct caaggtggga aggagagtaa tatgtcaacg
481 ttattagaaa gagctgtaga agcactacaa ggagaaaaac gatattatag tgatcctcga
541 tttctcaatc tctggcttaa attagggcgt ttatgcaatg agcctttgga tatgtacagt
601 tacttgcaca accaagggat tggtgtttca cttgctcagt tctatatctc atgggcagaa
661 gaatatgaag ctagagaaaa ctttaggaaa gcagatgcga tatttcagga agggattcaa
721 cagaaggctg aaccactaga aagactacag tcccagcacc gacaattcca agctcgagtg
781 tctcggcaaa ctctgttggc acttgagaaa gaagaagagg aggaagtttt tgagtcttct
841 gtaccacaac gaagcacact agctgaacta aagagcaaag ggaaaaagac agcaagagct
901 ccaatcatcc gtgtaggagg tgctctcaag gctccaagcc agaacagagg actccaaaat
961 ccatttcctc aacagatgca aaataatagt agaattactg tttttgatga aaatgctgat
1021 gaggctccta cagcagagtt gtctaagcct acagtccagc catggatagc accccccatg
1081 cccagggcca aagagaatga gctgcaagca ggcccttgga acacaggcag gtccttggaa
1141 cacaggcctc gtggcaatac agcttcactg atagctgtac ccgctgtgct tcccagtttc
1201 actccatatg tggaagagac tgcacaacag ccagttatga caccatgtaa aattgaacct
1261 agtataaacc acatcctaag caccagaaag cctggaaagg aagaaggaga tcctctacaa
1321 agggttcaga gccatcagca agcgtctgag gagaagaaag agaagatgat gtattgtaag
1381 gagaagattt atgcaggagt aggggaattc tcctttgaag aaattcgggc tgaagttttc
1441 cggaagaaat taaaagagca aagggaagcc gagctattga ccagtgcaga gaagagagca
1501 gaaatgcaga aacagattga agagatggag aagaagctaa aagaaatcca aactactcag
1561 caagaaagaa caggtgatca gcaagaagag acgatgccta caaaggagac aactaaactg
1621 caaattgctt ccgagtctca gaaaatacca ggaatgactc tatccagttc tgtttgtcaa
1681 gtaaactgtt gtgccagaga aacttcactt gcggagaaca tttggcagga acaacctcat
1741 tctaaaggtc ccagtgtacc tttctccatt tttgatgagt ttcttctttc agaaaagaag
1801 aataaaagtc ctcctgcaga tcccccacga gttttagctc aacgaagacc ccttgcagtt
1861 ctcaaaacct cagaaagcat cacctcaaat gaagatgtgt ctccagatgt ttgtgatgaa
1921 tttacaggaa ttgaaccctt gagcgaggat gccattatca caggcttcag aaatgtaaca
1981 atttgtccta acccagaaga cacttgtgac tttgccagag cagctcgttt tgtatccact
2041 ccttttcatg agataatgtc cttgaaggat ctcccttctg atcctgagag actgttaccg
2101 gaagaagatc tagatgtaaa gacctctgag gaccagcaga cagcttgtgg cactatctac
2161 agtcagactc tcagcatcaa gaagctgagc ccaattattg aagacagtcg tgaagccaca
2221 cactcctctg gcttctctgg ttcttctgcc tcggttgcaa gcacctcctc catcaaatgt
2281 cttcaaattc ctgagaaact agaacttact aatgagactt cagaaaaccc tactcagtca
2341 ccatggtgtt cacagtatcg cagacagcta ctgaagtccc taccagagtt aagtgcctct
2401 gcagagttgt gtatagaaga cagaccaatg cctaagttgg aaattgagaa ggaaattgaa
2461 ttaggtaatg aggattactg cattaaacga gaatacctaa tatgtgaaga ttacaagtta
2521 ttctgggtgg cgccaagaaa ctctgcagaa ttaacagtaa taaaggtatc ttctcaacct
2581 gtcccatggg acttttatat caacctcaag ttaaaggaac gtttaaatga agattttgat
2641 catttttgca gctgttatca atatcaagat ggctgtattg tttggcacca atatataaac
2701 tgcttcaccc ttcaggatct tctccaacac agtgaatata ttacccatga aataacagtg
2761 ttgattattt ataacctttt gacaatagtg gagatgctac acaaagcaga aatagtccat
2821 ggtgacttga gtccaaggtg tctgattctc agaaacagaa tccacgatcc ctatgattgt
2881 aacaagaaca atcaagcttt gaagatagtg gacttttcct acagtgttga ccttagggtg
2941 cagctggatg tttttaccct cagcggcttt cggactgtac agatcctgga aggacaaaag
3001 atcctggcta actgttcttc tccctaccag gtagacctgt ttggtatagc agatttagca
3061 catttactat tgttcaagga acacctacag gtcttctggg atgggtcctt ctggaaactt
3121 agccaaaata tttctgagct aaaagatggt gaattgtgga ataaattctt tgtgcggatt
3181 ctgaatgcca atgatgaggc cacagtgtct gttcttgggg agcttgcagc agaaatgaat
3241 ggggtttttg acactacatt ccaaagtcac ctgaacaaag ccttatggaa ggtagggaag
3301 ttaactagtc ctggggcttt gctctttcag tga
//