LOCUS AK312684 1292 bp mRNA linear HTC 24-MAY-2008
DEFINITION Homo sapiens cDNA, FLJ93078, Homo sapiens glutamic-oxaloacetic
transaminase 1, soluble(aspartate aminotransferase 1) (GOT1), mRNA.
ACCESSION AK312684
VERSION AK312684.1
KEYWORDS HTC; HTC_FLI; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1292)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Kaida,T., Tsuchiya,K.,
Iida,Y., Takayama,Y., Murakawa,K., Kanehori,K., Andoh,T.,
Kagawa,N., Sato,R., Kawamura,Y., Tanaka,S., Kisu,Y., Sugano,S.,
Goshima,N., Nomura,N. and Isogai,T.
TITLE NEDO functional analysis of protein and research application
project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC and Biological
Information Research Center (BIRC), AIST; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1292
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2014787"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000431550"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 51..1292
/note="Homo sapiens glutamic-oxaloacetic transaminase 1,
soluble(aspartate aminotransferase 1) (GOT1), mRNA"
/protein_id="BAG35564.1"
/translation="MAPPSVFAEVPQAQPVLVFKLTADFREDPDPRKVNLGVGAYRTD
DCHPWVLPVVKKVEQKIANDNSLNHEYLPILGLAEFRSCASRLALGDDSPALKEKRVG
GVQSLGGTGALRIGADFLARWYNGTNNKNTPVYVSSPTWENHNAVFSAAGFKDIRSYR
YWDAEKRGLDLQGFLNDLENAPEFSIVVLHACAHNPTGIDPTPEQWKQIASVMKHRFL
FPFFDSAYQGFASGNLERDAWAIRYFVSEGFEFFCAQSFSKNFGLYNERVGNLTVVGK
EPESILQVLSQMEKIVRITWSNPPAQGARIVASTLSNPELFEEWTGNVKTMADRILTM
RSELRARLEALKTPGTWNHITDQIGMFSFTGLNPKQVEYLVNEKHIYLLPSGRINVSG
LTTKNLDYVATSIHEAVTKIQ"
BASE COUNT 308 a 339 c 338 g 307 t
ORIGIN
1 ctgccagctc cgggcgtggg gtgaaatctc ttgattccta gtctctcgat atggcacctc
61 cgtcagtctt tgccgaggtt ccgcaggccc agcctgtcct ggtcttcaag ctcactgccg
121 acttcaggga ggatccggac ccccgcaagg tcaacctggg agtgggagca tatcgcacgg
181 atgactgcca tccctgggtt ttgccagtag tgaagaaagt ggagcagaag attgctaatg
241 acaatagcct aaatcacgag tatctgccaa tcctgggcct ggctgagttc cggagctgtg
301 cttctcgtct tgcccttggg gatgacagcc cagcactcaa ggagaagcgg gtaggaggtg
361 tgcaatcttt ggggggaaca ggtgcacttc gaattggagc tgatttctta gcgcgttggt
421 acaatggaac aaacaacaag aacacacctg tctatgtgtc ctcaccaacc tgggagaatc
481 acaatgctgt gttttccgct gctggtttta aagacattcg gtcctatcgc tactgggatg
541 cagagaagag aggattggac ctccagggct tcctgaatga tctggagaat gctcctgagt
601 tctccattgt tgtcctccac gcctgtgcac acaacccaac tgggattgac ccaactccgg
661 agcagtggaa gcagattgct tctgtcatga agcaccggtt tctgttcccc ttctttgact
721 cagcctatca gggcttcgca tctggaaacc tggagagaga tgcctgggcc attcgctatt
781 ttgtgtctga aggcttcgag ttcttctgtg cccagtcctt ctccaagaac ttcgggctct
841 acaatgagag agtcgggaat ctgactgtgg ttggaaaaga acctgagagc atcctgcaag
901 tcctttccca gatggagaag atcgtgcgga ttacttggtc caatcccccc gcccagggag
961 cacgaattgt ggccagcacc ctctctaacc ctgagctctt tgaggaatgg acaggtaatg
1021 tgaagacaat ggctgaccgg attctgacca tgagatctga actcagggca cgactagaag
1081 ccctcaaaac ccctgggacc tggaaccaca tcactgatca aattggcatg ttcagcttca
1141 ctgggttgaa ccccaagcag gttgagtatc tggtcaatga aaagcacatc tacctgctgc
1201 caagtggtcg aatcaacgtg agtggcttaa ccaccaaaaa tctagattac gtggccacct
1261 ccatccatga agcagtcacc aaaatccagt ga
//