LOCUS AK312255 2274 bp mRNA linear HTC 24-MAY-2008
DEFINITION Homo sapiens cDNA, FLJ92550, Homo sapiens heat shock 90kDa protein
1, beta (HSPCB), mRNA.
ACCESSION AK312255
VERSION AK312255.1
KEYWORDS HTC; HTC_FLI; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2274)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Kaida,T., Tsuchiya,K.,
Iida,Y., Takayama,Y., Murakawa,K., Kanehori,K., Andoh,T.,
Kagawa,N., Sato,R., Kawamura,Y., Tanaka,S., Kisu,Y., Sugano,S.,
Goshima,N., Nomura,N. and Isogai,T.
TITLE NEDO functional analysis of protein and research application
project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC and Biological
Information Research Center (BIRC), AIST; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2274
/cell_type="normal astrocytes (NHA5732)"
/clone="ASTRO2017270"
/clone_lib="ASTRO2"
/db_xref="H-InvDB:HIT000431121"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, normal astrocytes"
/organism="Homo sapiens"
CDS 100..2274
/note="Homo sapiens heat shock 90kDa protein 1, beta
(HSPCB), mRNA"
/protein_id="BAG35187.1"
/translation="MPEEVHHGEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELI
SNASDALDKIRYESLTDPSKLDSGKELKIDIIPNPQERTLTLVDTGIGMTKADLINNL
GTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVAEKVVVITKHNDDEQYAWESS
AGGSFTVRADHGEPIGRGTKVILHLKEDQTEYLEERRVKEVVKKHSQFIGYPITLYLE
KEREKEISDDEAEEEKGEKEEEDKDDEEKPKIEDVGSDEEDDSGKDKKKKTKKIKEKY
IDQEELNKTKPIWTRNPDDITQEEYGEFYKSLTNDWEDHLAVKHFSVEGQLEFRALLF
IPRRAPFDLFENKKKKNNIKLYVRRVFIMDSCDELIPEYLNFIRGVVDSEDLPLNISR
EMLQQSKILKVIRKNIVKKCLELFSELAEDKENYKKFYEAFSKNLKLGIHEDSTNRRR
LSELLRYHTSQSGDEMTSLSEYVSRMKETQKSIYYITGESKEQVANSAFVERVRKRGF
EVVYMTEPIDEYCVQQLKEFDGKSLVSVTKEGLELPEDEEEKKKMEESKAKFENLCKL
MKEILDKKVEKVTISNRLVSSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMMAKK
HLEINPDHPIVETLRQKAEADKNDKAVKDLVVLLFETALLSSGFSLEDPQTHSNRIYR
MIKLGLGIDEDEVAAEEPNAAVPDEIPPLEGDEDASRMEEVD"
BASE COUNT 664 a 487 c 596 g 527 t
ORIGIN
1 agctctctcg agtcactccg gcgcagtgtt gggactgtct gggtatcgga aagcaagcct
61 acgttgctca ctattacgta taatcctttt cttttcaaga tgcctgagga agtgcaccat
121 ggagaggagg aggtggagac ttttgccttt caggcagaaa ttgcccaact catgtccctc
181 atcatcaata ccttctattc caacaaggag attttccttc gggagttgat ctctaatgct
241 tctgatgcct tggacaagat tcgctatgag agcctgacag acccttcgaa gttggacagt
301 ggtaaagagc tgaaaattga catcatcccc aaccctcagg aacgtaccct gactttggta
361 gacacaggca ttggcatgac caaagctgat ctcataaata atttgggaac cattgccaag
421 tctggtacta aagcattcat ggaggctctt caggctggtg cagacatctc catgattggg
481 cagtttggtg ttggctttta ttctgcctac ttggtggcag agaaagtggt tgtgatcaca
541 aagcacaacg atgatgaaca gtatgcttgg gagtcttctg ctggaggttc cttcactgtg
601 cgtgctgacc atggtgagcc cattggcagg ggtaccaaag tgatcctcca tcttaaagaa
661 gatcagacag agtacctaga agagaggcgg gtcaaagaag tagtgaagaa gcattctcag
721 ttcataggct atcccatcac cctttatttg gagaaggaac gagagaagga aattagtgat
781 gatgaggcag aggaagagaa aggtgagaaa gaagaggaag ataaagatga tgaagaaaag
841 cccaagatcg aagatgtggg ttcagatgag gaggatgaca gcggtaagga taagaagaag
901 aaaactaaga agatcaaaga gaaatacatt gatcaggaag aactaaacaa gaccaagcct
961 atttggacca gaaaccctga tgacatcacc caagaggagt atggagaatt ctacaagagc
1021 ctcactaatg actgggaaga ccacttggca gtcaagcact tttctgtaga aggtcagttg
1081 gaattcaggg cattgctatt tattcctcgt cgggctccct ttgacctttt tgagaacaag
1141 aagaaaaaga acaacatcaa actctatgtc cgccgtgtgt tcatcatgga cagctgtgat
1201 gagttgatac cagagtatct caattttatc cgtggtgtgg ttgactctga ggatctgccc
1261 ctgaacatct cccgagaaat gctccagcag agcaaaatct tgaaagtcat tcgcaaaaac
1321 attgttaaga agtgccttga gctcttctct gagctggcag aagacaagga gaattacaag
1381 aaattctatg aggcattctc taaaaatctc aagcttggaa tccacgaaga ctccactaac
1441 cgccgccgcc tgtctgagct gctgcgctat catacctccc agtctggaga tgagatgaca
1501 tctctgtcag agtatgtttc tcgcatgaag gagacacaga agtccatcta ttacatcact
1561 ggtgagagca aagagcaggt ggccaactca gcttttgtgg agcgagtgcg gaaacggggc
1621 ttcgaggtgg tatatatgac cgagcccatt gacgagtact gtgtgcagca gctcaaggaa
1681 tttgatggga agagcctggt ctcagttacc aaggagggtc tggagctgcc tgaggatgag
1741 gaggagaaga agaagatgga agagagcaag gcaaagtttg agaacctctg caagctcatg
1801 aaagaaatct tagataagaa ggttgagaag gtgacaatct ccaatagact tgtgtcttca
1861 ccttgctgca ttgtgaccag cacctacggc tggacagcca atatggagcg gatcatgaaa
1921 gcccaggcac ttcgggacaa ctccaccatg ggctatatga tggccaaaaa gcacctggag
1981 atcaaccctg accaccccat tgtggagacg ctgcggcaga aggctgaggc cgacaagaat
2041 gataaggcag ttaaggacct ggtggtgctg ctgtttgaaa ccgccctgct atcttctggc
2101 ttttcccttg aggatcccca gacccactcc aaccgcatct atcgcatgat caagctaggt
2161 ctaggtattg atgaagatga agtggcagca gaggaaccca atgctgcagt tcctgatgag
2221 atcccccctc tcgagggcga tgaggatgcg tctcgcatgg aagaagtcga ttag
//