LOCUS AK312253 1684 bp mRNA linear HTC 24-MAY-2008
DEFINITION Homo sapiens cDNA, FLJ92548, highly similar to Homo sapiens
pyruvate kinase, muscle (PKM2), mRNA.
ACCESSION AK312253
VERSION AK312253.1
KEYWORDS HTC; HTC_FLI; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1684)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Kaida,T., Tsuchiya,K.,
Iida,Y., Takayama,Y., Murakawa,K., Kanehori,K., Andoh,T.,
Kagawa,N., Sato,R., Kawamura,Y., Tanaka,S., Kisu,Y., Sugano,S.,
Goshima,N., Nomura,N. and Isogai,T.
TITLE NEDO functional analysis of protein and research application
project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC and Biological
Information Research Center (BIRC), AIST; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1684
/cell_type="normal astrocytes (NHA5732)"
/clone="ASTRO2017257"
/clone_lib="ASTRO2"
/db_xref="H-InvDB:HIT000431119"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, normal astrocytes"
/organism="Homo sapiens"
CDS 89..1684
/note="highly similar to Homo sapiens pyruvate kinase,
muscle (PKM2), mRNA"
/protein_id="BAG35185.1"
/translation="MSKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARN
TGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDP
ILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLD
YKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDL
PAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEG
VRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESM
IKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHL
QLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPI
IAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDV
VIVLTGWRPGSGFTNTMRVVPVP"
BASE COUNT 380 a 445 c 505 g 354 t
ORIGIN
1 gcacagcagc tgcacgcgcc gtggctccgg atctcttcgt ctttgcagcg tagcccgagt
61 cggtcagcgc cggaggacct cagcagccat gtcgaagccc catagtgaag ccgggactgc
121 cttcattcag acccagcagc tgcacgcagc catggctgac acattcctgg agcacatgtg
181 ccgcctggac attgattcac cacccatcac agcccggaac actggcatca tctgtaccat
241 tggcccagct tcccgatcag tggagacgtt gaaggagatg attaagtctg gaatgaatgt
301 ggctcgtctg aacttctctc atggaactca tgagtaccat gcggagacca tcaagaatgt
361 gcgcacagcc acggaaagct ttgcttctga ccccatcctc taccggcccg ttgctgtggc
421 tctagacact aaaggacctg agatccgaac tgggctcatc aagggcagcg gcactgcaga
481 ggtggagctg aagaagggag ccactctcaa aatcacgctg gataacgcct acatggaaaa
541 gtgtgacgag aacatcctgt ggctggacta caagaacatc tgcaaggtgg tggaagtggg
601 cagcaagatc tacgtggatg atgggcttat ttctctccag gtgaagcaga aaggtgccga
661 cttcctggtg acggaggtgg aaaatggtgg ctccttgggc agcaagaagg gtgtgaacct
721 tcctggggct gctgtggact tgcctgctgt gtcggagaag gacatccagg atctgaagtt
781 tggggtcgag caggatgttg atatggtgtt tgcgtcattc atccgcaagg catctgatgt
841 ccatgaagtt aggaaggtcc tgggagagaa gggaaagaac atcaagatta tcagcaaaat
901 cgagaatcat gagggggttc ggaggtttga tgaaatcctg gaggccagtg atgggatcat
961 ggtggctcgt ggtgatctag gcattgagat tcctgcagag aaggtcttcc ttgctcagaa
1021 gatgatgatt ggacggtgca accgagctgg gaagcctgtc atctgtgcta ctcagatgct
1081 ggagagcatg atcaagaagc cccgccccac tcgggctgaa ggcagtgatg tggccaatgc
1141 agtcctggat ggagccgact gcatcatgct gtctggagaa acagccaaag gggactatcc
1201 tctggaggct gtgcgcatgc agcacctaat tgcccgtgag gcagaggctg ccatctacca
1261 cttgcaatta tttgaggaac tccgccgcct ggcgcccatt accagcgacc ccacagaagc
1321 caccgccgtg ggtgccgtgg aggcctcctt caagtgctgc agtggggcca taatcgtcct
1381 caccaagtct ggcaggtctg ctcaccaggt ggccagatac cgcccacgtg cccccatcat
1441 tgctgtgacc cggaatcccc agacagctcg tcaggcccac ctgtaccgtg gcatcttccc
1501 tgtgctgtgc aaggacccag tccaggaggc ctgggctgag gacgtggacc tccgggtgaa
1561 ctttgccatg aatgttggca aggcccgagg cttcttcaag aagggagatg tggtcattgt
1621 gctgaccgga tggcgccctg gctccggctt caccaacacc atgcgtgttg ttcctgtgcc
1681 gtga
//