LOCUS AK309703 2760 bp mRNA linear HTC 12-JAN-2008
DEFINITION Homo sapiens cDNA, FLJ99744.
ACCESSION AK309703
VERSION AK309703.1
KEYWORDS HTC; HTC_FLI; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2760)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2760
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3009240"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000428569"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
BASE COUNT 757 a 720 c 686 g 597 t
ORIGIN
1 aaaacacagc ctgccaatcc tggagaatgc ctggtggggg tgctggggga agaagtccaa
61 tttcagctgg gaaaatggga caggagcctt gggagtgggt ttacacctca aattccctta
121 tggccacagt ccattggccc ctagcctcct ccccacttta cagatgaaga gactgaggtt
181 tagagagagg aacagccggc ttggcagagg caactgctga ggcagttgag gaaagtgaat
241 tttgaagatt gcaccggtcg acaaaggaca gcctattttt ccctcgacac ccgattcaaa
301 gtgggcacag atggtgtgat tacagtcaaa aggcctctac ggtttcataa cccacagatc
361 catttcttgg tctacgcctg ggactccacc tacagaaagt tttccaccaa agtcacgctg
421 aatacagtgg ggcaccacca ccgccccccg ccccatcagg cctccgtttc tggaatccaa
481 gcagaattgc tcacatttcc caactcctct cctggcctca gaagacagaa gagagactgg
541 gttattcctc ccatcagctg cccagaaaat gaaaaaggcc catttcctaa aaacctggtt
601 cagatcaaat ccaacaaaga caaagaaggc aaggttttct acagcatcac tggccaagga
661 gctgacacac cccctgttgg tgtctttatt attgaaagag aaacaggatg gctgaaggtg
721 acagagcctc tggatagaga acgcattgcc acatacactc tcttctctca cgctgtgtca
781 tccaacggga atgcagttga ggatccaatg gagattttga tcacggtaac cgatcagaat
841 gacaacaagc ccgaattcac ccaggaggtc tttaaggggt ctgtcatgga aggtgctctt
901 ccaggaacct ctgtgatgga ggtcacagcc acagacgcgg acgatgatgt gaacacctac
961 aatgccgcca tcgcttacac catcctcagc caagatcctg agctccctga caaaaatatg
1021 ttcaccatta acaggaacac aggagtcatc agtgtggtca ccactgggct ggaccgagag
1081 agtttcccta cgtataccct ggtggtccaa gctgctgacc ttcaaggtga ggggttaagc
1141 acaacagcaa cagctgtgat cacagtcact gacaccaacg ataatcctcc gatcttcaat
1201 cccaccacgt acaagggtca ggtgcctgag aacgaggcta acgtcgtaat caccacactg
1261 aaagtgactg atgctgatgc ccccaatacc ccagcgtggg aggctgtata caccatattg
1321 aatgatgatg gtggacaatt tgtcgtcacc acaaatccag tgaacaacga tggcattttg
1381 aaaacagcaa agggcttgga ttttgaggcc aagcagcagt acattctaca cgtagcagtg
1441 acgaatgtgg taccttttga ggtctctctc accacctcca cagccaccgt caccgtggat
1501 gtgctggatg tgaatgaagc ccccatcttt gtgcctcctg aaaagagagt ggaagtgtcc
1561 gaggactttg gcgtgggcca ggaaatcaca tcctacactg cccaggagcc agacacattt
1621 atggaacaga aaataacata tcggatttgg agagacactg ccaactggct ggagattaat
1681 ccggacactg gtgccatttc cactcgggct gagctggaca gggaggattt tgagcacgtg
1741 aagaacagca cgtacacagc cctaatcata gctacagaca atggttctcc agttgctact
1801 ggaacaggga cacttctgct gatcctgtct gatgtgaatg acaacgcccc cataccagaa
1861 cctcgaacta tattcttctg tgagaggaat ccaaagcctc aggtcataaa catcattgat
1921 gcagaccttc ctcccaatac atctcccttc acagcagaac taacacacgg ggcgagtgcc
1981 aactggacca ttcagtacaa cgacccaacc caagaatcta tcattttgaa gccaaagatg
2041 gccttagagg tgggtgacta caaaatcaat ctcaagctca tggataacca gaataaagac
2101 caagtgacca ccttagaggt cagcctgtgt gactgtgaag gggccgctgg cgtctgtagg
2161 aaggcacagc ctgtcgaagc aggattgcaa attcctgcca ttctggggat tcttggagga
2221 attcttgctt tgctaattct gattctgctg ctcttgctgt ttcttcggag gagagcggtg
2281 gtcaaagagc ccttactgcc cccagaggat gacacccggg acaacgttta ttactatgat
2341 gaagaaggag gcggagaaga ggaccaggac tttgacttga gccagctgca caggggcctg
2401 gacgctcggc ctgaagtgac tcgtaacgac gttgcaccaa ccctcatgag tgtcccccgg
2461 tatcttcccc gccctgccaa tcccgatgaa attggaaatt ttattgatga aaatctgaaa
2521 gcggctgata ctgaccccac agccccgcct tatgattctc tgctcgtgtt tgactatgaa
2581 ggaagcggtt ccgaagctgc tagtctgagc tccctgaact cctcagagtc agacaaagac
2641 caggactatg actacttgaa cgaatggggc aatcgcttca agaagctggc tgacatgtac
2701 ggaggcggcg aggacgacta ggggactcga gagaggcggg ccccagaccc atgtgctggg
//