LOCUS AK309700 1578 bp mRNA linear HTC 12-JAN-2008
DEFINITION Homo sapiens cDNA, FLJ99741.
ACCESSION AK309700
VERSION AK309700.1
KEYWORDS HTC; HTC_FLI; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1578)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1578
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3005643"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000428566"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
BASE COUNT 379 a 525 c 379 g 295 t
ORIGIN
1 gtttacctaa tcaagcctgg gcaatggcgg gcgcccctcc cccagcctcg ctgccgcctt
61 gcagtttgat ctcagactgc tgtgctagca atcagggaga ctctgtgggc gtaggaccct
121 ccgagccagg tgcgggatat aatcttgtgg tgccacgttt tttaagccag tccgaaaagc
181 gcaatattcg ggtgggagtg acccgatttt ccaggtgcgt ctgtcacccc tttctttgac
241 tcggaaaggg aactccctga ccccttgcgc ttcccaagtg aggcaatgcc tcgccctgct
301 tcggctcgcg cacggtgcgc gcacccactg acctgcgccc actgtctgga actccctagt
361 gagaagaacc cggtacctca gatggaaatg cagaaatcac ccgtcttctg cgtcgctcac
421 gctgggagct gtggactgga gctgttccta cgcggccatc ttggctcctc cctagatagg
481 atccaatgat agctggacaa gtcagtaagc ccttgctgtc agtgcggagt gaaatgaatg
541 cggagttgag aggtgaggac aaggctgcta cttcagacag cgagctgaat gagcccctgc
601 ttgcgcctgt ggaatcaaat gacagcgagg acactcccag caagctcttc ggggctagag
661 gaaacccagc attatcagac ccaggcactc ctgaccaaca ccaggccagt cagacccacc
721 ccccatttcc agttgggcca cagccacttc tgacggcaca gcagttagct tctgctgtgg
781 ccggcgtgat gccgggaggc cccccagccc tcaaccagcc aatcctcatt cccttcaaca
841 tggcgggaca gctaggaggc cagcaaggac tggttctcac actgccaaca gcgaatctca
901 ccaacatcca agggctggtg gcagcagctg cagccggagg cattatgact ctgccactgc
961 aaaatctaca agctgcctca tccctgaact cccagctcca gcagctccag ctccagctcc
1021 agcagcagca gcagcagcag cagcagcagc ctcccccgtc aaccaaccag cacccgcaac
1081 cagccccaca ggcgccctcg cagtcccagc agcagccgct gcagcccacc ccaccccagc
1141 agccaccacc cgcctctcag cagccgccag ctcctacatc tcagctgcaa caggcgcctc
1201 agccccagca gcaccaaccc cactcccact cccggaacca gaaccaacca tctccaaccc
1261 agcagagctc cagccccccg cagaaaccta gtcagtctcc aggacatggc ctgccttcac
1321 cgctcacgcc acccaatcct ctacagatcc tgcacccaag gaggagtatg gactaaggga
1381 gaaaggtgga tggaagaatt tggtacttca cttcagaaat gattcagaac caagcacaca
1441 gaaagtggag aactgcctac ctagtctagc taactgtatg catttactgg agaagcaata
1501 attttacaaa gaggctagga actggtctcc ttattgaata aagaataagc tcccagattt
1561 acattcattg attttcta
//