LOCUS AK309695 2324 bp mRNA linear HTC 12-JAN-2008
DEFINITION Homo sapiens cDNA, FLJ99736.
ACCESSION AK309695
VERSION AK309695.1
KEYWORDS HTC; HTC_FLI; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2324)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2324
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3000117"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000428561"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
BASE COUNT 547 a 662 c 594 g 521 t
ORIGIN
1 ctctttttta gaagcagcga tcggagatgg atgtctctct ttgcccagcc aagtgtagtt
61 tctggcggat tttcttgctg ggaagcgtct ggctggacta tgtgggctcc gtgctggctt
121 gccctgcaaa ttgtgtctgc agcaagactg agatcaattg ccggcggccg gacgatggga
181 acctcttccc cctcctggaa gggcaggatt cagggaacag caatgggaac gccagtatca
241 acatcacgga catctcaagg aatatcactt ccatacacat agagaactgg cgcagtcttc
301 acacgctcaa cgccgtggac atggagctct acaccggact tcaaaagctg accatcaaga
361 actcaggact tcggagcatt cagcccagag cctttgccaa gaacccccat ttgcgttata
421 taaacctgtc aagtaaccgg ctcaccacac tctcgtggca gctcttccag acgctgagtc
481 ttcgggaatt gcagttggag cagaactttt tcaactgcag ctgtgacatc cgctggatgc
541 agctctggca ggagcagggg gaggccaagc tcaacagcca gaacctctac tgcatcaacg
601 ctgatggctc ccagcttcct ctcttccgca tgaacatcag tcagtgtgac cttcctgaga
661 tcagcgtgag ccacgtcaac ctgaccgtac gagagggtga caatgctgtt atcacttgca
721 atggctctgg atcacccctt cctgatgtgg actggatagt cactgggctg cagtccatca
781 acactcacca gaccaatctg aactggacca atgttcatgc catcaacttg acgctggtga
841 atgtgacgag tgaggacaat ggcttcaccc tgacgtgcat tgcagagaac gtggtgggca
901 tgagcaatgc cagtgttgcc ctcactgtct actatccccc acgtgtggtg agcctggagg
961 agcctgagct gcgcctggag cactgcatcg agtttgtggt gcgtggcaac cccccaccaa
1021 cgctgcactg gctgcacaat gggcagcctc tgcgggagtc caagatcatc catgtggaat
1081 actaccaaga gggagagatt tccgagggct gcctgctctt caacaagccc acccactaca
1141 acaatggcaa ctataccctc attgccaaaa acccactggg cacagccaac cagaccatca
1201 atggccactt cctcaaggag ccctttccag ttgacgaagt gagtcccaca cctcctatca
1261 ctgtgaccca caaaccagaa gaagacactt ttggggtatc catagcagtt gggcttgctg
1321 cttttgcctg tgtcctgttg gtggttctct tcgtcatgat caacaaatat ggtcgacggt
1381 ccaaatttgg aatgaagggt cccgtggctg tcatcagtgg tgaggaggac tcagccagcc
1441 cactgcacca catcaaccac ggcatcacca cgccctcgtc actggatgcc gggcccgaca
1501 ctgtggtcat tggcatgact cgcatccctg tcattgagaa cccccagtac ttccgtcagg
1561 gacacaactg ccacaagccg gacacgtatg tgcagcacat taagaggaga gacatcgtgc
1621 tgaagcgaga actgggtgag ggagcctttg gaaaggtctt cctggccgag tgctacaacc
1681 tcagcccgac caaggacaag atgcttgtgg ctgtgaaggc cctgaaggat cccaccctgg
1741 ctgcccggaa ggatttccag agggaggccg agctgctcac caacctgcag catgagcaca
1801 ttgtcaagtt ctatggagtg tgcggcgatg gggaccccct catcatggtc tttgaataca
1861 tgaagcatgg agacctgaat aagttcctca gggcccatgg gccagatgca atgatccttg
1921 tggatggaca gccacgccag gccaagggtg agctggggct ctcccaaatg ctccacattg
1981 ccagtcagat cgcctcgggt atggtgtacc tggcctccca gcactttgtg caccgagacc
2041 tggccaccag gaactgcctg gttggagcga atctgctagt gaagattggg gacttcggca
2101 tgtccagaga tgtctacagc acggattatt acagggtaaa gtgactttcc cagctgcagg
2161 actcagccac agagctagcg ccttgcttac atgtcttttt cattgtccat taaccctgtc
2221 actctggcac acacatatac acacccatgc ccagacatgc actcaacagt tatcttttgg
2281 cctctcttgc atcttgacat acctgtttat tatgttctca attg
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