LOCUS AK309692 1948 bp mRNA linear HTC 12-JAN-2008
DEFINITION Homo sapiens cDNA, FLJ99733.
ACCESSION AK309692
VERSION AK309692.1
KEYWORDS HTC; HTC_FLI; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1948)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1948
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2027060"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000428558"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
BASE COUNT 441 a 529 c 575 g 403 t
ORIGIN
1 actgtgtcct cgtggccctg cctgggaaca ggctttggca ggctttggca ggcttcgtgc
61 cctgggactt gctcaggcca gtgcctgcct gtctggagag gactctgata ccatgggctg
121 tgcccctgct gtagacgggg ctgcaggctg ccggggctgg ggatgggctg tggctccagc
181 agccacgggg cagaagacgt ccccacctgg ctttgcaagt cggcttcttt attgtgcttt
241 gttatgcttg tcgtcacgca gctgggctat gacacacgtg tgaccatcct cgggcacgtg
301 cagagaggag ggaccccttc ggcattcgac aggatcttgg ccagccgcat gggagtggag
361 gcagtcatcg ccttgctaga ggccaccccg gacaccccag cttgcgtcgt gtcactgaac
421 gggaaccacg ccgtgcgcct gccgctgatg gagtgcgtgc agatgactca ggatgtgcag
481 aaggcgatgg acgagaggag atttcaagat gcggttcgac tccgagggag gagctttgcg
541 ggcaacctga acacctacaa gcgacttgcc atcaagctgc cggacgatca gatcccaaag
601 accaattgca acgtagctgt catcaacgtg ggggcacccg cggctgggat gaacgcggcc
661 gtacgctcag ctgtgcgcgt gggcattgcc gacggccaca ggatgctcgc catctatgat
721 ggctttgacg gcttcgccaa gggccagatc aaagaaatcg gctggacaga tgtcgggggc
781 tggaccggcc aaggaggctc cattcttggg acaaaacgcg ttctcccggg gaagtacttg
841 gaagagatcg ccacacagat gcgcacgcac agcatcaacg cgctgctgat catcggtgga
901 ttcgaggcct acctgggact cctggagctg tcagccgccc gggagaagca cgaggagttc
961 tgtgtcccca tggtcatggt tcccgctact gtgtccaaca atgtgccggg ttccgatttc
1021 agcatcgggg cagacaccgc cctgaacact atcaccgaca cctgcgaccg catcaagcag
1081 tccgccagcg gaaccaagcg gcgcgtgttc atcatcgaga ccatgggcgg ctactgtggc
1141 tacctggcca acatgggggg gctcgcggct ggagctgatg ccgcatacat ttttgaagag
1201 cccttcgaca tcagggatct gcagtccaac gtggagcacc tgacggagaa aatgaagacc
1261 accatccaga gaggccttgt gctcagaaat gagagctgca gtgaaaacta caccaccgac
1321 ttcatttacc agctgtattc agaagagggc aaaggcgtgt ttgactgcag gaagaacgtg
1381 ctgggtcaca tgcagcaggg tggggcaccc tctccatttg atagaaactt tggaaccaaa
1441 atctctgcca gagctatgga gtggatcact gcaaaactca aggaggcccg gggcagagga
1501 aaaaaattta ccaccgatga ttccatttgt gtgctgggaa taagcaaaag aaacgttatt
1561 tttcaacctg tggcagagct gaagaagcaa acggattttg agcacaggat tcccaaagaa
1621 cagtggtggc tcaagctacg gcccctcatg aaaatcctgg ccaagtacaa ggccagctat
1681 gacgtgtcgg actcaggcca gctggaacat gtgcagccct ggagtgtctg acccagtccc
1741 gcctgcatgt gcctgcagcc accgtggact gtcagttttt gtaacactta agttatttta
1801 tcagcacttt atgcacgtat tattgacatt aatacctaat cggcgagtgc ccatctgccc
1861 caccagctcc agtgcgtgct gtctgtggag tgtgtctcat gctttcagat gtgcatatga
1921 gcagaattaa acatttgcct acaactcc
//