LOCUS AK309689 1319 bp mRNA linear HTC 12-JAN-2008
DEFINITION Homo sapiens cDNA, FLJ99730.
ACCESSION AK309689
VERSION AK309689.1
KEYWORDS HTC; HTC_FLI; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1319)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1319
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2022185"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000428555"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
BASE COUNT 436 a 210 c 238 g 435 t
ORIGIN
1 atgactggca gtggcatcag cgatggcggc tgcgtcgggg tcggttctgc agcgctgtat
61 cgtgtcgccg gcagggaggc atagcgcctc tctgatcttc ctgcatggct caggtgattc
121 tggacaagga ttaagaatgt ggatcaagca ggttttaaat caagatttaa cattccaaca
181 cataaaaatt atttatccaa cagctcctcc cagtatgttt ttgcttttag gaggattctc
241 tatgggagga tgcatggcaa tgcatttagc atatagaaat catcaagatg tggcaggagt
301 atttgctctt tctagttttc tgaataaagc atctgctgtt taccaggctc ttcagaagag
361 taatggtgta cttcctgaat tatttcagtg tcatggtact gcagatgagt tagttcttca
421 ttcttgggca gaagagacaa actcaatgtt aaaatctcta ggagtgacca cgaagtttca
481 tagttttcca aatgtttacc atgagctaag caaaactgag ttagacatat tgaagttatg
541 gattcttaca aagctgccag gagaaatgga aaaacaaaaa tgaatgaatc aagagtgatt
601 tgttaatgta agtgtaatgt ctttgtgaaa agtgattttt actgccaaat tataatgata
661 attaaaatat taagaaataa cactttcctg acttttttat tattaaaatg cttatcactg
721 tagacagtag ctaatcttat taatgaaaaa caatagacaa acatctgtgc ataatttttc
781 agacacaatt ctgtaaatat ttggaaacct tttaagtatt taaactttta aatttttgaa
841 ataaagtatt ctaaactaat ataaataagg acaatgaaaa aacatgaaag gacttagcat
901 aatgttattt tatcttttct acaactttgt ttaaattacc tttccaaaga tatttgtgtt
961 tatgtaattt tccacggaat aacattaata ctctaggttt ataaaccggt ttcacattat
1021 ttcatttgat catcacaaga gctttgtgaa gtaagccgag aagttgttac tggtatttaa
1081 taatagcaat agaggagtta aagactttcc cacagcttgc aggtcaagac aagaaattca
1141 ggtctcctaa ttctcagtgg agctctattt ctgttaaccc aaattgctgc tctgttttag
1201 gtctcaattt catctgtaaa atgatactaa tagtacttat cccattggat ttttgttgag
1261 atttaaataa atagccaaaa gccaatacat aataaacact caataaagat taaccataa
//