LOCUS AK309678 1141 bp mRNA linear HTC 12-JAN-2008
DEFINITION Homo sapiens cDNA, FLJ99719.
ACCESSION AK309678
VERSION AK309678.1
KEYWORDS HTC; HTC_FLI; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1141)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1141
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2013376"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000428544"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
BASE COUNT 220 a 323 c 308 g 290 t
ORIGIN
1 agtaccggta gcccagtcga gatggaggaa gatgcgaccg gcagacggca ttcgctggga
61 acgacggata gactgggggc tgcggcctag aggtccgggc ttggagttcg cctcagacgc
121 ggtggagccg ccggagtctg agaagagcag ctctcctacc tgtgtcctaa ggagtgccct
181 cacattgtct ttcaagctgt ctcggggccc ctggctgcag ccactgcctc catcctcacc
241 aatcccatgg atgtcatacg aacccgtgtg caggttgagg gcaagaactc catcatcctg
301 accttcagac agctgatggc agaagaaggg ccttggggcc tcatgaaggg cctctcggcc
361 agaatcatct cagccacacc ttccaccatt gtcattgtgg tgggctatga gagcctcaag
421 aaactcagcc tccgacctga gctggtggac tcgagacact ggtaaccagt ggtggggaga
481 gaagcctgct gttttccaca ctaccgtggg tcaggggcag agtggagagg acagcaccct
541 ctccaggtgc tcccaccaca cacccagccc tgccctgggc caagtggcct atctgggata
601 gggatagaga ctttgaactg ctcttgctga agaggctcca cgcctggatc ccttgccccc
661 actatttaaa attctcttct gagctgggct ccctcactca gtccctgtat ttgatactgg
721 cctaaagacc ccacccccca ccctgccagc ccttcttctg gcttcccctt ccatctgtgt
781 ccctgagacc ctgagaagag ctgtacatag agcttgctta ctaccactgg ttcttcctct
841 tgggctttca gcccagactc caagcagctg ctatcaaccc tctctccctt catctcttag
901 ccttgcttat ttttattttg ggaccgagct gcccactaga tgactctgct tttccctgca
961 tttggggcta aggtgccagg tacttatttg cacagggagc aggagcagca aaaaatctct
1021 ggttctccag agcactcgtc ctctctttgg aggggttatt aggttgggag aaatgttgat
1081 acttttgttt tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtt
1141 t
//