LOCUS AK309674 2757 bp mRNA linear HTC 12-JAN-2008
DEFINITION Homo sapiens cDNA, FLJ99715.
ACCESSION AK309674
VERSION AK309674.1
KEYWORDS HTC; HTC_FLI; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2757)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2757
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2011843"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000428540"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
BASE COUNT 851 a 662 c 602 g 642 t
ORIGIN
1 gtccggggtc agtcagtcgc tccctgtcgc tgccggagag tctctgcttc ccccttccta
61 cgcgctccgc ggcggtagct cgggctctcc ggaggagggg gcgacagagt aaaagagagg
121 ctgacttaat aaagtttcct gaaacaacaa ctaccaccga aaaggcccaa ggttggggga
181 gacccccatt tttctccgcc cctctcctaa agcctgaggg accgttgcag gtggagcggc
241 gctggtggga cccgggttgc ggcggcagcc gttgccccgg cgagggaagc ctcaggtcct
301 gcccgctccg gttccggctc cttcggtggt ggcggcggca gctgctgccg ggcggttcct
361 cctgtcaact gcctccgccc gcgcgcctcc cgcctagcca cacagcgcgg aggacgccgt
421 gagccgccgc cgcagtcgtc tcggcccgcg gagggacgtg gtgagctgtg gcgcttccgc
481 gtcagcgccg gcggggactc tttgggagcg cgtcgggccc gagtccgggt tcccccgcgt
541 ggtcagcgga ggctgcgcca ggtggggccc gcagccgcct cccacgccag gctgtccccg
601 cggccgcccg gcctgccttc cttccttccg cccgcccgcc ggcccgccct aagagttgac
661 cacgccgcaa tgaagagctt cttacagtga aagcaaagta ggtcgagtcc actgaaaatg
721 cctttaaggg acaaatactg tcagactgac caccatcatc acggatgctg tgaaccagtt
781 tatatcctgg aacctggaga tcctcctttg ttacagcaac cactacagac atccaaatct
841 ggtattcaac aaataattga gtgctttcga tcaggaacta aacaacttaa gcatatttta
901 ttaaaagatg tggacactat ttttgaatgt aagttatgcc gcagtctctt cagaggatta
961 ccaaatttaa ttacccataa aaaattctac tgcccaccaa gtctccagat ggatgacaac
1021 cttcctgatg taaatgataa acaaagccaa gccataaatg atctcctaga agccatatat
1081 ccaagtgtgg acaaacgaga atatattatt aagctagaac ccatagaaac taatcaaaat
1141 gcagtatttc aatatatttc gaggactgat aatcctattg aagtcacaga gtcaagcagt
1201 actcctgaac aaaccgaagt tcagatacag gaaactagca ctgaacagtc aaaaacagta
1261 ccggttacag atacagaggt ggaaactgta gagccccctc ctgttgagat tgttacagat
1321 gaagttgcac ctacatctga tgaacaacct caggagtcgc aggctgactt ggaaacttct
1381 gacaattctg attttggtca ccagttgata tgttgtcttt gtagaaaaga attcaattct
1441 agacgaggtg ttcgccgtca cattcgaaaa gtacacaaga aaaagatgga agaactaaaa
1501 aagtacattg aaacacgaaa gaatccaaac caatcctcta aaggacgcag taagaatgtt
1561 ctagttccat taagtaggag ttgtccagta tgttgtaaat catttgctac aaaagcgaat
1621 gtaaggaggc attttgatga agttcataga ggactaagga gggattcaat tactcctgat
1681 atagcaacaa agcctgggca acctttgttc ctggattcta tttctcctaa aaaatctttt
1741 aagactcgaa aacaaaagtc ttcttcaaag gctgaataca atttaactgc atgcaaatgc
1801 ctcctttgca agaggaaata tagttcacaa ataatgctta aaagacatat gcaaattgtc
1861 cacaagataa ctctttctgg aacaaactct aaaagagaaa aaggccctaa taatactgcc
1921 aacagttcag aaataaaagt taaagttgaa ccagcagatt ctgtagaatc ttcaccccct
1981 tccattaccc attctccaca gaatgaatta aagggaacaa atcattcaaa tgaaaaaaag
2041 aacacaccgg cagcacagaa aaataaagtt aaacaagact ctgaaagccc taaatcaact
2101 agtccgtcgg ctgcaggtgg ccagcaaaaa accagaaaac caaaactttc agctggcttt
2161 gactttaagc aactttactg taaactttgt aaacgtcagt ttacttccaa acagaacttg
2221 actaaacaca tcgagttgca cacagatgga aataacattt atgttaaatt ctacaagtgt
2281 cctctttgca cttatgaaac tcgtcggaaa cgtgatgtga tacgacatat aactgtggtt
2341 cataaaaagt catctcgtta tcttgggaaa ataacagcca gtttagagat cagagctata
2401 aaaaagccta ttgattttgt tctaaataaa gtggcaaaaa gaggcccttc gagggatgaa
2461 gcaaaacata gtgattcaaa acatgatggc acttctaact ctcctagtaa aaagtatgaa
2521 gtagctgacg tcggtattga agtaaaagtc acaaaaaact tttctcttca cagatgcaat
2581 aaatgtggaa aggcatttgc caaaaagact taccttgaac atcataagaa aactcataag
2641 gcaaatgctt ccaattcacc tgaaggaaac aaaaccaaag gccgaagtac aagatctaag
2701 gctcttgtct ggtgaggaac agttaacaga gttttgcttt tttcccccca tcgaact
//