LOCUS AK308082 2286 bp mRNA linear HTC 12-JAN-2008
DEFINITION Homo sapiens cDNA, FLJ98030.
ACCESSION AK308082
VERSION AK308082.1
KEYWORDS HTC; HTC_FLI; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2286)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2286
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3008138"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000426948"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
BASE COUNT 633 a 517 c 556 g 580 t
ORIGIN
1 acttcccagc cggggccagt cgggagcgaa agtgcgctga gctgcagtgt ctggtcgaga
61 gtacccgtgg gagcgtcgcg ccgcggaggc agccgtcccg gcgtaggtgg cgtggccgac
121 cggaccccca actggcgcct ctccccgcgc ggggtcccga gctaggagat gggaggcaca
181 gctcgtgggc ctgggcggaa ggatgcgggg ccgcctgggg ccgggctccc gccccagcag
241 cggaggttgg gggatggtgt ctatgacacc ttcatgatga tagatgaaac caaatgtccc
301 ccctgttcaa atgtactctg caatccttct gaaccacctc cacccagaag actaaatatg
361 accactgagc agtttacagg agatcatact cagcactttt tggatggagg tgagatgaag
421 gtagaacagc tgtttcaaga atttggcaac agaaaatcca atactattca gtcagatggc
481 atcagtgact ctgaaaaatg ctctcctact gtttctcagg gtaaaagttc agattgcttg
541 aatacagtaa aatccaacag ttcatccaag gcacccaaag tggtgcctct gactccagaa
601 caagccctga agcaatataa acaccacctc actgcctatg agaaactgga aataattaat
661 tatccagaaa tttactttgt aggtccaaat gccaagaaaa gacatggagt tattggtggt
721 cccaataatg gagggtatga tgatgcagat ggggcctata ttcatgtacc tcgagaccat
781 ctagcttatc gatatgaggt gctgaaaatt attggcaagg ggagttttgg gcaggtggcc
841 agggtctatg atcacaaact tcgacagtac gtggccctaa aaatggtgcg caatgagaag
901 cgctttcatc gtcaagcagc tgaggagatc cggattttgg agcatcttaa gaaacaggat
961 aaaactggta gtatgaacgt tatccacatg ctggaaagtt tcacattccg gaaccatgtt
1021 tgcatggcct ttgaattgct gagcatagac ctttatgagc tgattaaaaa aaataagttt
1081 cagggtttta gcgtccagtt ggtacgcaag tttgcccagt ccatcttgca atctttggat
1141 gccctccaca aaaataagat tattcactgc gatctgaagc cagaaaacat tctcctgaaa
1201 caccacgggc gcagttcaac caaggtcatt gactttgggt ccagctgttt cgagtaccag
1261 aagctctaca catatatcca gtctcggttc tacagagctc cagaaatcat cttaggaagc
1321 cgctacagca caccaattga catatggagt tttggctgca tccttgcaga acttttaaca
1381 ggacagcctc tcttccctgg agaggatgaa ggagaccagt tggcctgcat gatggagctt
1441 ctagggatgc caccaccaaa acttctggag caatccaaac gtgccaagta ctttattaat
1501 tccaagggca taccccgcta ctgctctgtg actacccagg cagatgggag ggttgtgctt
1561 gtggggggtc gctcacgtag gggtaaaaag cggggtcccc caggcagcaa agactggggg
1621 acagcactga aagggtgtga tgactacttg tttatagagt tcttgaaaag gtgtcttcac
1681 cgggacccct ctgcccgctt gaccccagct caagcattaa gacacccttg gattagcaag
1741 tctgtcccca gacctctcac caccatagac aaggtgtcag ggaaacgggt agttaatcct
1801 gcaagtgctt tctagggatt gggttctaag ctgcctccag ttgttggaat agccaataag
1861 cttaaagcta acttaatgtc agaaaccaat ggtagtatac ccctatgcag tgtattgcca
1921 aaactgatta gctagtggac agagatatgc ccagagatgc atatgtgtat atttttatga
1981 tcttacaaac ctgcaaatgg aaaaaatgca agcccattgg tggatgtttt tgttagagta
2041 gacttttttt aaacaagaca aaacattttt atatgattat aaaagaattc ttcaagggct
2101 aattacctaa ccagcttgta ttggccatct ggaatatgca ttaaatgact ttttataggt
2161 caatgcatct ttgttattat cgtcagatgt atttcaactg atgtattata ctattggttt
2221 aaatctcttt ctctcaagat agaaggtgta gcaaaagtat cccaactact cctcgcttct
2281 agtgtc
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