LOCUS AK307576 2827 bp mRNA linear HTC 12-JAN-2008
DEFINITION Homo sapiens cDNA, FLJ97524.
ACCESSION AK307576
VERSION AK307576.1
KEYWORDS HTC; HTC_FLI; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2827)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2827
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2023921"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000426442"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
BASE COUNT 806 a 677 c 739 g 605 t
ORIGIN
1 tattcatatg ctgctgctgc tgcagccacc agagcagaga gccttgagta cactttgcca
61 cagaaagcag tgagcaagaa tgatagctgt ctcttttaaa tgccgttgtc aaattctgag
121 gcgacttact aaagatgaga gtccctacac taaatccgcc agccagacaa agccgcctga
181 tggagcgttg gctgtgagga gacagagcat cccagaggaa ttcaagggct ccacagtcgt
241 cgagctgatg aagaaggaag gcactaccct gggtctgacg gtatcgggag gaattgataa
301 ggatggcaag ccaagagtat ctaatctgcg gcaaggagga attgctgcta gaagtgacca
361 gctggatgtg ggtgactaca tcaaagcagt gaatggaatc aacctggcca aattccgcca
421 tgacgagatc atcagcttgc tgaagaatgt gggagaaaga gtggttcttg aagtagagta
481 cgagcttcca ccggtctctg tgcaaggatc aagtgttatt ttccgaacag tggaggtcac
541 attacataaa gaaggcaata cctttggttt tgtaattcga gggggagcac atgatgatag
601 aaataaatct cgtccagttg tgataacatg tgttcgtcct ggagggcctg ctgacagaga
661 gggcacgatc aaacccggtg acaggttgct cagtgtggat ggaattcggc ttcttggaac
721 tacgcatgct gaagccatga gtattcttaa acaatgtgga caagaagcag cactgctgat
781 agaatatgat gtctcagtaa tggactctgt ggcaacagca tccgggccac tactagtcga
841 agttgccaaa actcctggtg ccagccttgg ggttgcccta actacctcga tgtgctgtaa
901 caaacaagtc attgtcatag acaaaatcaa atctgcaagt attgcagaca gatgtggcgc
961 attgcatgtg ggagatcaca tcctctccat cgatggaacc agcatggagt actgtacact
1021 tgcagaagca acccagttcc tggccaacac cactgaccag gtcaagcttg agatccttcc
1081 ccatcatcag acccggctgg ccctaaaggg gcccgaccat gcagctttgg tgtcttcatc
1141 cttctctcct acctccatga gtgcatacag cctgagttcc ctgaacatgg ggactctacc
1201 tcgaagcctc tactccacca gcccacgtgg aaccatgatg aggaggagac tgaaaaagaa
1261 agacttcaaa agctcattgt ccttagcctc cagcacagta ggattggctg ggcaggttgt
1321 tcacacagaa accacagagg ttgtgctgac ggcagatcct gtcacaggat ttgggatcca
1381 actgcagggc agtgtgtttg ccacagaaac tctctcttct ccacctctga tttcctatat
1441 cgaagctgac agcccagcag agagatgtgg ggtgctacag attggagaca gagtgatggc
1501 catcaatgga attccaacag aagacagcac cttcgaagaa gccagtcagc tcctccgaga
1561 ctcttcaatc acgagcaagg tcacactgga aatcgagttt gatgttgcag agtctgtcat
1621 cccaagtagt ggaacatttc atgtaaagct gcctaagaag cacaatgtgg aacttggaat
1681 aaccataagt tcaccatcca gtagaaaacc aggagacccc ctcgtcattt cagatatcaa
1741 gaaagggagt gtggcacaca gaactgggac cctggaactt ggggataaat tgctcgcaat
1801 agataatatc cggctggaca actgttccat ggaagatgca gttcagatcc tccagcaatg
1861 tgaagacctg gtgaagctca aaatccgcaa agatgaagat aattcagatg agcaagaaag
1921 ttccggagca attatttaca ccgtggagct taaacgctac ggggggcccc ttggcatcac
1981 aatttcagga actgaagagc cgtttgatcc tataatcatt tcaagcctca ctaaaggggg
2041 attagctgaa agaactggcg caatccacat aggagaccga atcctagcca tcaatagcag
2101 cagcttgaaa gggaagcctc tgagtgaagc catccatttg ttacagatgg caggagagac
2161 tgtcaccttg aaaattaaga aacagacaga tgcccagtca gcatcgagcc ccaagaagtt
2221 ccctatttct agccatttga gtgacctggg ggatgtggag gaggactcct caccagcaca
2281 gaagccaggc aagctctccg acatgtaccc ctccacggtg cccagtgtgg acagtgctgt
2341 ggattcatgg gatgggtctg caatagacac cagctatgga actgaaggca ctagttttca
2401 ggcctcagga tacaatttca acacctatga ctggaggagt ccaaaacaga gaggcagctt
2461 gtccccagtc actaagcctc gaagccagac ttacccagat gtggggctga gttatgaaga
2521 ctgggaccgg tccacagcca gtggttttgc aggggctgcc gatagtgcag agacagaaca
2581 agaggagaac ttctggtctc aagcgctgga ggatttggaa acctgcggac agtcaggaat
2641 tctgagagaa ctggaggcaa caatcatgtc ggggagcacg atgagtttga atcatgaggc
2701 tccaacacct cgcagtcagc tggggcgaca ggccagcttc caggagcgca gcagctcgcg
2761 gccgcactac agccaaacaa ctcggagcaa caccctgcct tcagatgtgg gtaggaagtc
2821 aacctcc
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