LOCUS AK307250 1596 bp mRNA linear HTC 12-JAN-2008
DEFINITION Homo sapiens cDNA, FLJ97198.
ACCESSION AK307250
VERSION AK307250.1
KEYWORDS HTC; HTC_FLI; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1596)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1596
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2021580"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000426116"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
BASE COUNT 416 a 341 c 419 g 420 t
ORIGIN
1 agagctggag agccgcggct gggaccggag tggggagcgc ggcgtggagg tgccacccgg
61 cgcgggtggc ggagagatca gaagcctctt ccccaagccg agccaacctc agcggggacc
121 cgggctcagg gacgcggcgg cggcggcggc gactgcagtg gctggacgat ggcagcgtcc
181 gccggagccg gggcggtgat tgcagcccca gacagccggc gctggctgtg gtcggtgctg
241 gcggcggcgc ttgggctctt gacagctgga gtatcagcct tggaagtata tacgccaaaa
301 gaaatcttcg tggcaaatgg tacacaaggg aagctgacct gcaagttcaa gtctactagt
361 acgactggcg ggttgacctc agtctcctgg agcttccagc cagagggggc cgacactact
421 gtgtcgagaa tttgcctgtg tttccagttt gggtagtggt gggcatagtt actgctgtgg
481 tcctaggtct cactctgctc atcagcatga ttctggctgt cctctataga aggaaaaact
541 ctaaacggga ttacactggc tgcagtacat cagagagttt gtcaccagtt aagcaggctc
601 ctcggaagtc cccctccgac actgagggtc ttgtaaagag tctgccttct ggatctcacc
661 agggcccagt catatatgca cagttagacc actccggcgg acatcacagt gacaagatta
721 acaagtcaga gtctgtggtg tatgcggata tccgaaagaa ttaagagaat acctagaaca
781 tatcctcagc aagaaacaaa accaaactgg actctcgtgc agaaaatgta gcccattacc
841 acatgtagcc ttggagaccc aggcaaggac aagtacacgt gtactcacag agggagagaa
901 agatgtgtac aaaggatatg tataaatatt ctatttagtc atcctgatat gaggagccag
961 tgttgcatga tgaaaagatg gtatgattct acatatgtac ccattgtctt gctgtttttg
1021 tactttcttt tcaggtcatt tacaattggg agatttcaga aacattcctt tcaccatcat
1081 ttagaaatgg tttgccttaa tggagacaat agcagatcct gtagtatttc cagtagacat
1141 ggccttttaa tctaagggct taagactgat tagtcttagc atttactgta gttggaggat
1201 ggagatgcta tgatggaagc atacccaggg tggcctttag cacagtatca gtaccattta
1261 tttgtctgcc gcttttaaaa aatacccatt ggctatgcca cttgaaaaca atttgagaag
1321 tttttttgaa gtttttctca ctaaaatatg gggcaattgt tagccttaca tgttgtgtag
1381 acttacttta agtttgcacc cttgaaatgt gtcatatcaa tttctggatt cataatagca
1441 agattagcaa aggataaatg ccgaaggtca cttcattctg gacacagttg gatcaatact
1501 gattaagtag aaaatccaag ctttgcttga gaacttttgt aacgtggaga gtaaaaagta
1561 tcggttttat tctttgctga tgtcctttct gcttga
//