LOCUS AK304401 4460 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ61727 partial cds, highly similar to Laminin
alpha-4 chain precursor.
ACCESSION AK304401
VERSION AK304401.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4460)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..4460
/clone="TRACH3034509"
/clone_lib="TRACH3"
/db_xref="H-InvDB:HIT000499025"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="trachea"
CDS 222..>4460
/codon_start=1
/note="highly similar to Laminin alpha-4 chain precursor"
/protein_id="BAH14174.1"
/transl_table=1
/translation="MALSSAWRSVLPLWLLWSAACSRAASGDDNAFPFDIEGSSAVGR
QDPPETSEPRVALGRLPPAAEKCNAGFFHTLSGECVPCDCNGNSNECLDGSGYCVHCQ
RNTTGEHCEKCLDGYIGDSIRGAPQFCQPCPCPLPHLANFAESCYRKNGAVRCICNEN
YAGPNCERCAPGYYGNPLLIGSTCKKCDCSGNSDPNLIFEDCDEVTGQCRNCLRNTTG
FKCERCAPGYYGDARIAKNCAVCNCGGGPCDSVTGECLEEGFEPPTGMDCPTISCDKC
VWDLTDDLRLAALSIEEGKSGVLSVSSGAAAHRHVNEINATIYLLKTKLSERENQYAL
RKIQINNAENTMKSLLSDVEELVEKENQASRKGQLVQKESMDTINHASQLVEQAHDMR
DKIQEINNKMLYYGEEHELSPKEISEKLVLAQKMLEEIRSRQPFFTQRELVDEEADEA
YELLSQAESWQRLHNETRTLFPVVLEQLDDYNAKLSDLQEALDQALNHVRDAEDMNRA
TAARQRDHEKQQERVREQMEVVNMSLSTSADSLTTPRLTLSELDDIIKNASGIYAEID
GAKSELQVKLSNLSNLSHDLVQEAIDHAQDLQQEANELSRKLHSSDMNGLVQKALDAS
NVYENIVNYVSEANETAEFALNTTDRIYDAVSGIDTQIIYHKDESENLLNQARELQAK
AESSSDEAVADTSRRVGGALARKSALKTRLSDAVKQLQAAERGDAQQRLGQSRLITEE
ANRTTMEVQQATAPMANNLTNWSQNLQHFDSSAYNTAVNSARDAVRNLTEVVPQLLDQ
LRTVEQKRPASNVSASIQRIRELIAQTRSVASKIQVSMMFDGQSAVEVHSRTSMDDLK
AFTSLSLYMKPPVKRPELTETADQFILYLGSKNAKKEYMGLAIKNDNLVYVYNLGTKD
VEIPLDSKPVSSWPAYFSIVKIERVGKHGKVFLTVPSLSSTAEEKFIKKGEFSGDDSL
LDLDPEDTVFYVGGVPSNFKLPTSLNLPGFVGCLELATLNNDVISLYNFKHIYNMDPS
TSVPCARDRLAFTQSRAASYFFDGSGYAVVRDITRRGKFGQVTRFDIEVRTPADNGLI
LLMVNGSMFFRLEMRNGYLHVFYDFGFSSGPVHLEDTLKKAQINDAKYHEISIIYHND
KKMILVVDRRHVKSMDNEKMKIPFTDIYIGGAPPEILQSRALRAHLPLDINFRGCMKG
FQFQKKDFNLLEQTETLGVGYGCPEDSLISRRAYFNGQSFIASIQKISFFDGFEGGFN
FRTLQPNGLLFYYASGSDVFSISLDNGTVIMDVKGIKVQSVDKQYNDGLSHFVISSVS
PTRYELIVDKSRVGSKNPTKGKIEQTQASEKKFYFGGSPISAQYANFTGCISNAYFTR
VDRDVEVEDFQRYTEKVHTSLYECPIESSPLFLLH"
BASE COUNT 1283 a 1032 c 1132 g 1013 t
ORIGIN
1 agtcccgaac ggccagggag aggaggtggc ctagcgctgg cggggctcac cccaatccgt
61 ctgccttttg atgccgtact ctgctggttg cgcagccacc tcgggatact gcacacggag
121 aggagggaaa ataagcgagg caccgccgca ccacgcggga gacctacgga gacccacagc
181 gcccgagccc tggaagagcg ctactggatg tcagcggaga aatggctttg agctcagcct
241 ggcgctcggt tctgcctctg tggctcctct ggagcgctgc ctgctcccgc gccgcgtccg
301 gggacgacaa cgcttttcct tttgacattg aagggagctc agcggttggc aggcaagacc
361 cgcctgagac gagcgaaccc cgcgtggctc tgggacgcct gccgcctgcg gccgagaaat
421 gcaatgctgg attctttcac accctgtcgg gagaatgtgt gccctgcgac tgtaatggca
481 attccaacga gtgtttggac ggctcaggat actgtgtgca ctgccagcgg aacacaacag
541 gagagcactg tgaaaagtgt ctggatggtt atatcggaga ttccatcagg ggagcacccc
601 aattctgcca gccgtgcccc tgtcccctgc cccacttggc caattttgca gaatcctgct
661 ataggaaaaa tggagctgtt cggtgcattt gtaacgaaaa ttatgctgga cctaactgtg
721 aaagatgtgc tcccggttac tatggaaacc ccttactcat tggaagcacc tgtaagaaat
781 gtgactgcag tggaaattca gatcccaacc tgatctttga agattgtgat gaagtcactg
841 gccagtgtag gaattgctta cgcaacacca ccggattcaa gtgtgaacgt tgcgctcctg
901 gctactatgg ggacgccagg atagccaaga actgtgcagt gtgcaactgc gggggaggcc
961 catgtgacag tgtaaccgga gaatgcttgg aagaaggttt tgaaccccct acaggcatgg
1021 actgcccaac cataagctgt gataagtgcg tctgggacct gactgatgac ctgcggttag
1081 cagcgctctc catcgaggaa ggcaaatccg gggtgctgag cgtatcctct ggggccgccg
1141 ctcataggca cgtgaatgaa atcaacgcca ccatctacct cctcaaaaca aaattgtcag
1201 aaagagaaaa ccaatacgcc ctaagaaaga tacaaatcaa caatgctgag aacacgatga
1261 aaagccttct gtctgacgta gaggaattag ttgaaaagga aaatcaagcc tccagaaaag
1321 gacaacttgt tcagaaggaa agcatggaca ccattaacca cgcaagtcag ctggtagagc
1381 aagcccatga tatgagggat aaaatccaag agatcaacaa caagatgctc tattatgggg
1441 aagagcatga acttagcccc aaggaaatct ctgagaagct ggtgttggcc cagaagatgc
1501 ttgaagagat tagaagccgt caaccatttt tcacccaacg ggagctcgtg gatgaggagg
1561 cagatgaggc ttacgaacta ctgagccagg ctgagagctg gcagcggctg cacaatgaga
1621 cccgcactct gtttcctgtc gtcctggagc agctggatga ctacaacgct aagttgtcag
1681 atctccagga agcacttgac caggccctta accatgtcag ggatgccgaa gacatgaaca
1741 gggccacagc agccaggcag cgggaccatg agaaacaaca ggaaagagtg agggaacaaa
1801 tggaagtggt gaacatgtct ctgagcacat ctgcggactc tctgacaaca cctcgtctaa
1861 ctctttcaga acttgatgat ataataaaga atgcgtcagg gatttatgca gaaatagatg
1921 gagccaaaag cgaactacaa gtaaaactat ctaacctaag taacctcagc catgatttag
1981 tccaagaagc tattgaccat gcacaggacc ttcaacaaga agctaatgaa ttgagcagga
2041 agttgcacag ttcagatatg aacgggctgg tacagaaggc tttggatgca tcaaatgtct
2101 atgaaaatat tgttaattat gttagtgaag ccaatgaaac agcagaattt gctttgaaca
2161 ccactgaccg aatttatgat gcggtgagtg ggattgatac tcaaatcatt taccataaag
2221 atgaaagtga gaacctcctc aatcaagcca gagaactgca agcaaaggca gagtctagca
2281 gtgatgaagc agtggctgac actagcaggc gtgtgggtgg agccctagca aggaaaagtg
2341 cccttaaaac cagactcagt gatgccgtta agcaactaca agcagcagag agaggggatg
2401 cccagcagcg cctggggcag tctagactga tcaccgagga agccaacagg acgacgatgg
2461 aggtgcagca ggccactgcc cccatggcca acaatctaac caactggtca cagaatcttc
2521 aacattttga ctcttctgct tacaacactg cagtgaactc tgctagggat gcagtaagaa
2581 atctgaccga ggttgtccct cagctcctgg atcagcttcg tacggttgag cagaagcgac
2641 ctgcaagcaa cgtttctgcc agcatccaga ggatccgaga gctcattgct cagaccagaa
2701 gtgttgccag caagatccaa gtctccatga tgtttgatgg ccagtcagct gtggaagtgc
2761 actcgagaac cagtatggat gacttaaagg ccttcacgtc tctgagcctg tacatgaaac
2821 cccctgtgaa gcggccggaa ctgaccgaga ctgcagatca gtttatcctg tacctcggaa
2881 gcaaaaacgc caaaaaagag tatatgggtc ttgcaatcaa aaatgataat ctggtatacg
2941 tctataattt gggaactaaa gatgtggaga ttcccctgga ctccaagccc gtcagttcct
3001 ggcctgctta cttcagcatt gtcaagattg aaagggtggg aaaacatgga aaggtgtttt
3061 taacagtccc gagtctaagt agcacagcag aggaaaagtt cattaaaaag ggggaatttt
3121 cgggagatga ctctctgctg gacctggacc ctgaggacac agtgttttat gttggtggag
3181 tgccttccaa cttcaagctc cctaccagct taaacctgcc tggctttgtt ggctgcctgg
3241 aactggccac tttgaataat gatgtgatca gcttgtacaa ctttaagcac atctataata
3301 tggacccctc cacatcagtg ccatgtgccc gagataggct ggccttcact cagagtcggg
3361 ctgccagtta cttcttcgat ggctccggtt atgccgtggt gagagacatc acaaggagag
3421 ggaaatttgg tcaggtgact cgctttgaca tagaagttcg aacaccagct gacaacggcc
3481 ttattctcct gatggtcaat ggaagtatgt ttttcagact ggaaatgcgc aatggttacc
3541 tacatgtgtt ctatgatttt ggattcagca gtggccctgt gcatcttgaa gatacgttaa
3601 agaaagctca aattaatgat gcaaaatacc atgagatctc aatcatttac cacaatgata
3661 agaaaatgat cttggtagtt gacagaaggc atgtcaagag catggataat gaaaagatga
3721 aaataccctt tacagatata tacattggag gagctcctcc agaaatctta caatccaggg
3781 ccctcagagc acaccttccc ctagatatca acttcagagg atgcatgaag ggcttccagt
3841 tccaaaagaa ggacttcaat ttactggagc agacagaaac cctgggagtt ggttatggat
3901 gcccagaaga ctcacttata tctcgcagag catatttcaa tggacagagc ttcattgctt
3961 caattcagaa aatatctttc tttgatggct ttgaaggagg ttttaatttc cgaacattac
4021 aaccaaatgg gttactattc tattatgctt cagggtcaga cgtgttctcc atctcactgg
4081 ataatggtac tgtcatcatg gatgtaaagg gaatcaaagt tcagtcagta gataagcagt
4141 acaatgatgg gctgtcccac ttcgtcatta gctctgtctc acccacaaga tatgaactga
4201 tagtagataa aagcagagtt gggagtaaga atcctaccaa agggaaaata gaacagacac
4261 aagcaagtga aaagaagttt tacttcggtg gctcaccaat cagtgctcag tatgctaatt
4321 tcactggctg cataagtaat gcctacttta ccagggtgga tagagatgtg gaggttgaag
4381 atttccaacg gtatactgaa aaggtccaca cttctcttta tgagtgtccc attgagtctt
4441 caccattgtt tctcctccat
//