LOCUS AK304288 2228 bp mRNA linear HUM 31-JUL-2008
DEFINITION Homo sapiens cDNA FLJ58227 complete cds, highly similar to
Glutamate--cysteine ligase catalytic subunit (EC 6.3.2.2).
ACCESSION AK304288
VERSION AK304288.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2228)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2228
/clone="TRACH3024760"
/clone_lib="TRACH3"
/db_xref="H-InvDB:HIT000498912"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="trachea"
CDS 172..1926
/codon_start=1
/note="highly similar to Glutamate--cysteine ligase
catalytic subunit (EC 6.3.2.2)"
/protein_id="BAG65146.1"
/transl_table=1
/translation="MLVSFDHENKKVRLVLSGEKVLETLQEKGERTNPNHPTLWRPEY
GSYMIEGTPGQPYGGTMSEFNTVEANMRKRRKEATSILEENQALCTITSFPRLGCPGF
TLPEVKPNPVEGGASKSLFFPDEAINKHPRFSTLTRNIRHRRGEKVVINVPIFKDKNT
PSPFIETFTEDDEASRASKPDHIYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLAT
ICPIVMALSAASPFYRGYVSDIDCRWGVISASVDDRTREERGLEPLKNNNYRISKSRY
DSIDSYLSKCGEKYNDIDLTIDKEIYEQLLQEGIDHLLAQHVAHLFIRDPLTLFEEKI
HLDDANESDHFENIQSTNWQTMRFKPPPPNSDIGWRVEFRPMEVQLTDFENSAYVVFV
VLLTRVILSYKLDFLIPLSKVDENMKVAQKRDAVLQGMFYFRKDICKGGNAVVDGCGK
AQNSTELAAEEYTLMSIDTIINGKEGVFPGLIPILNSYLENMEVDVDTRCSILNYLKL
IKKRASGELMTVARWMREFIANHPDYKQDSVITDEMNYSLILKCNQIANELCECPELL
GSAFRKVKYSGSKTDSSN"
BASE COUNT 681 a 444 c 538 g 565 t
ORIGIN
1 aggagcggcg agctagcgga cgcaaagact gggcatgctc cgcggcggcg caggttttgg
61 tcacaagtag gaagaagcca gtgcaccaga ccggcaaaga gaagcgggag ccgccgcggc
121 agcgcggccg tggggtccgc cgccgccgca tcggagcggg aggtggaata catgttggta
181 tcttttgatc atgaaaataa aaaagtccgg ttggtcctgt ctggggagaa agttcttgaa
241 actctgcaag agaaggggga aaggacaaac ccaaaccatc ctaccctttg gagaccagag
301 tatgggagtt acatgattga agggacacca ggacagccct acggaggaac aatgtccgag
361 ttcaatacag ttgaggccaa catgcgaaaa cgccggaagg aggctacttc tatattagaa
421 gaaaatcagg ctctttgcac aataacttca tttcccagat taggctgtcc tgggttcaca
481 ctgcccgagg tcaaacccaa cccagtggaa ggaggagctt ccaagtccct cttctttcca
541 gatgaagcaa taaacaagca ccctcgcttc agtaccttaa caagaaatat ccgacatagg
601 agaggagaaa aggttgtcat caatgtacca atatttaagg acaagaatac accatctcca
661 tttatagaaa catttactga ggatgatgaa gcttcaaggg cttctaagcc ggatcatatt
721 tacatggatg ccatgggatt tggaatgggc aattgctgtc tccaggtgac attccaagcc
781 tgcagtatat ctgaggccag atacctttat gatcagttgg ctactatctg tccaattgtt
841 atggctttga gtgctgcatc tcccttttac cgaggctatg tgtcagacat tgattgtcgc
901 tggggagtga tttctgcatc tgtagatgat agaactcggg aggagcgagg actggagcca
961 ttgaagaaca ataactatag gatcagtaaa tcccgatatg actcaataga cagctattta
1021 tctaagtgtg gtgagaaata taatgacatc gacttgacga tagataaaga gatctacgaa
1081 cagctgttgc aggaaggcat tgatcatctc ctggcccagc atgttgctca tctctttatt
1141 agagacccac tgacactgtt tgaagagaaa atacacctgg atgatgctaa tgagtctgac
1201 cattttgaga atattcagtc cacaaattgg cagacaatga gatttaagcc ccctcctcca
1261 aactcagaca ttggatggag agtagaattt cgacccatgg aggtgcaatt aacagacttt
1321 gagaactctg cctatgtggt gtttgtggta ctgctcacca gagtgatcct ttcctacaaa
1381 ttggattttc tcattccact gtcaaaggtt gatgagaaca tgaaggtagc acagaaaaga
1441 gatgctgtct tgcagggaat gttttatttc aggaaagata tttgcaaagg tggcaatgca
1501 gtggtggatg gttgtggcaa ggcccagaac agcacggagc tcgctgcaga ggagtacacc
1561 ctcatgagca tagacaccat catcaatggg aaggaaggtg tgtttcctgg actgatccca
1621 attctgaact cttaccttga aaacatggaa gtggatgtgg acaccagatg tagtattctg
1681 aactacctaa agctaattaa gaagagagca tctggagaac taatgacagt tgccagatgg
1741 atgagggagt ttatcgcaaa ccatcctgac tacaagcaag acagtgtcat aactgatgaa
1801 atgaattata gccttatttt gaagtgtaac caaattgcaa atgaattatg tgaatgccca
1861 gagttacttg gatcagcatt taggaaagta aaatatagtg gaagtaaaac tgactcatcc
1921 aactagacat tctacagaaa gaaaaatgca ttattgacga actggctaca gtaccatgcc
1981 tctcagccag cccgtgtgta taatatgaag accaaatgat agaactgtac tgttttctgg
2041 gccagtgagc cagaaattga ttaaggcttt ctttggtagg taaatctaga gtttatacag
2101 tgtacatgta catagtaaag tatttttgat taacaatgta ttttaataac atatctaaag
2161 tcatcatgaa ctggcttgta catttttaaa ttcttactct ggagcaacct actgtctaag
2221 cagttttg
//