LOCUS AK303941 2222 bp mRNA linear HUM 31-JUL-2008
DEFINITION Homo sapiens cDNA FLJ57294 complete cds, highly similar to
Lipoamide acyltransferase component of branched-chain alpha-keto
acid dehydrogenase complex, mitochondrial precursor (EC 2.3.1.168).
ACCESSION AK303941
VERSION AK303941.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2222)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2222
/clone="TRACH2016620"
/clone_lib="TRACH2"
/db_xref="H-InvDB:HIT000498565"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="trachea"
CDS 748..1653
/codon_start=1
/note="highly similar to Lipoamide acyltransferase
component of branched-chain alpha-keto acid dehydrogenase
complex, mitochondrial precursor (EC 2.3.1.168)"
/protein_id="BAG64869.1"
/transl_table=1
/translation="MENNIKLSEVVGSGKDGRILKEDILNYLEKQTGAILPPSPKVEI
MPPPPKPKDMTVPILVSKPPVFTGKDKTEPIKGFQKAMVKTMSAALKIPHFGYCDEID
LTELVKLREELKPIAFARGIKLSFMPFFLKAASLGLLQFPILNASVDENCQNITYKAS
HNIGIAMDTEQGLIVPNVKNVQICSIFDIATELNRLQKLGSVGQLSTTDLTGGTFTLS
NIGSIGGTFAKPVIMPPEVAIGALGSIKAIPRFNQKGEVYKAQIMNVSWSADHRVIDG
ATMSRFSNLWKSYLENPAFMLLDLK"
BASE COUNT 709 a 421 c 468 g 624 t
ORIGIN
1 attttttccg gggtaagatg gctgcagtcc gtatgctgag aacctggagc aggaatgcgg
61 ggaagctgat gctcctgcct tggtgacccc gatcttacaa aaactttcaa gagcctgcta
121 tgtaccagtg ctgtgttagg cactggagat acaaatatga aatagacatc atatcttcct
181 tctcaagaga ttacaacctt gtggtggagg acaaaggaga gggagacaga tagaaattac
241 aacccagcat gagagatttg tgttcgctat tttcaaacat gtggtaacgt tcatgttttg
301 aagccaaatt atgtgtgttt ctttggttat ccttcattca agtatagtca tccacatcac
361 ttcctgaaaa caactgctgc tctccgtgga caggttgttc agttcaagct ctcagacatt
421 ggagaaggga ttagagaagt aactgttaaa gaatggtatg taaaagaagg agatacagtg
481 tctcagtttg atagcatctg tgaagttcaa agtgataaag cttctgttac catcactagt
541 cgttatgatg gagtcattaa aaaactctat tataatctag acgatattgc ctatgtgggg
601 aagccattag tagacataga aacggaagct ttaaaagatt cagaagaaga tgttgttgaa
661 actcctgcag tgtctcatga tgaacataca caccaagaga taaagggccg aaaaacactg
721 gcaactcctg cagttcgccg tctggcaatg gaaaacaata ttaagctgag tgaagttgtt
781 ggctcaggaa aagatggcag aatacttaaa gaagatatcc tcaactattt ggaaaagcag
841 acaggagcta tattgcctcc ttcacccaaa gttgaaatta tgccacctcc accaaagcca
901 aaagacatga ctgttcctat actagtatca aaacctccgg tattcacagg caaagacaaa
961 acagaaccca taaaaggctt tcaaaaagca atggtcaaga ctatgtctgc agccctgaag
1021 atacctcatt ttggttattg tgatgagatt gaccttactg aactggttaa gctccgagaa
1081 gaattaaaac ccattgcatt tgctcgtgga attaaactct cctttatgcc tttcttctta
1141 aaggctgctt ccttgggatt actacagttt cctatcctta acgcttctgt ggatgaaaac
1201 tgccagaata taacatataa ggcttctcat aacattggga tagcaatgga tactgagcag
1261 ggtttgattg tccctaatgt gaaaaatgtt cagatctgct ctatatttga catcgccact
1321 gaactgaacc gcctccagaa attgggctct gtgggtcagc tcagcaccac tgatcttaca
1381 ggaggaacat ttactctttc caacattgga tcaattggtg gtacctttgc caaaccagtg
1441 ataatgccac ctgaagtagc cattggggcc cttggatcaa ttaaggccat tccccgattt
1501 aaccagaaag gagaagtata taaggcacag ataatgaatg tgagctggtc agctgatcac
1561 agagttattg atggtgctac aatgtcacgc ttctccaatt tgtggaaatc ctatttagaa
1621 aacccagctt ttatgctact agatctgaaa tgaagactga taagacattc ttgaactttt
1681 tgagcttcca aagagtatgt aaaccctagc tgtgccagca catgttcatc tttacaattt
1741 atattgtaaa tgatttgtat cgtatgatta aggatctaag gcacaatatt tgtcactgtt
1801 ctattagact ttttactgaa aatgaataat ggtgtaatgg ttctcctggg gctgtcacat
1861 tttataggtc agagtgtgac ttcttaatat ggtgctgatg tttttgtgtc aatggcttga
1921 aactggcaga gcaacacttt gtctcagaaa aaaaaaaaaa accaaaaagc caagtgtggt
1981 ggtgtgcacc tatagtccca gctactcagg aagctgagac aagaggatca attgagccca
2041 ggagttcaaa gctgtagtga gctgtcattg tgccactatc ctccagtatg ggtgacagag
2101 tgagacctgg tctctaaaaa ataaaataaa ataaaaaaaa caggtcaaat aaatgctgtt
2161 gttgtaaaat ttcagataat acaaagagtt aaccaataaa agaaaaagtc attcataatc
2221 tt
//