LOCUS AK303396 2347 bp mRNA linear HUM 31-JUL-2008
DEFINITION Homo sapiens cDNA FLJ58559 complete cds, highly similar to
Probable ATP-dependent RNA helicase DHX35 (EC 3.6.1.-).
ACCESSION AK303396
VERSION AK303396.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2347)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2347
/clone="THYMU3015956"
/clone_lib="THYMU3"
/db_xref="H-InvDB:HIT000498020"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="thymus"
CDS 25..2043
/codon_start=1
/note="highly similar to Probable ATP-dependent RNA
helicase DHX35 (EC 3.6.1.-)"
/protein_id="BAG64452.1"
/transl_table=1
/translation="MAAPVGPVKFWRPGTEGPGVSISEERQSLAENSGITVVYNPYAA
LSIEQQRQKLPVFKYLAEAGWTAEGRVVGVTQPRRVAAVTVAGRVAEERGAVLGHEVG
YCIRFDDCTDQLATRIKFLTDGMLVREMMVDPLLTKYSVIMLDEAHERTLYTDIAIGL
LKKIQKKRGDLRLIVASATLDADKFRDFFNQNETSDPARDTCVILTVEGRTFPVDIFY
LQSPVPDYIKSTVETVVKIHQTEGDGDVLAFLTGQEEVETVVSMLIEQARALARTGMK
RHLRVLPMYAGLPSFEQMKVFERVSRSVRKVIVATNVAETSITISGIVYVIDCGFVKL
RAYNPRTAIECLVVVPVSQASANQRAGRGGRSRSGKCYRLYTEEAFDKLPQSTVPEMQ
RSNLAPVILQLKALGIDNVLRFHFMSPPPAQSMVQALELLYALGGLDKDCRLTEPLGM
RIAEFPLNPMFAKMLLESGNFGCSQEILSIAAMMQIQNIFVVPPNQKSHAIRVHRKFA
VEEGDHLTMLNIYEAFIKHNKDSKWCQEHFLNYKGLVRAATVREQLKKLLVKFQVPRK
SSEGDPDLVLRCIVSGFFANAARFHSTGAYRTIRDDHELHIHPASVLYAEKPPRWVIY
NEVIQTSKYYMRDVTAIESAWLLELAPHFYQQGTHLSLKAKRAKVQDP"
BASE COUNT 584 a 562 c 626 g 575 t
ORIGIN
1 agcctcgtga ccttttaccc caacatggct gcgcccgtgg gaccggtgaa gttctggcga
61 cccggtacag aggggccagg tgtaagcatc tctgaagaga gacaaagtct ggctgaaaac
121 tctgggataa cggttgttta caacccttat gctgcccttt ccatagagca gcagaggcag
181 aagctgccgg tattcaagta ccttgcagaa gccggctgga cagctgaagg aagagtggta
241 ggagtgaccc agcctcgaag agtggctgct gttacagttg cagggagagt agctgaagaa
301 aggggtgcag tgctgggcca cgaggtgggc tactgcatcc gctttgatga ctgcaccgac
361 cagctggcca cgagaattaa gtttcttact gatggaatgc tggtcaggga aatgatggtt
421 gatccgttgt taacaaaata tagtgtcatc atgctggatg aagcccacga gaggaccttg
481 tacactgaca ttgccattgg cttgctaaaa aagattcaga aaaagcgagg ggatcttcga
541 ttgattgtag cttcagccac tctggatgca gacaaattcc gggatttctt taatcaaaat
601 gaaaccagtg atccagcaag ggatacatgt gtgatcctta cagtggaagg gagaacattt
661 ccggtggata tcttttatct acaaagtcct gttccagatt atatcaaatc aactgtcgaa
721 actgtggtga aaattcacca gacagaggga gacggagacg ttttagcatt tcttactggc
781 caggaagagg tagaaactgt tgtgtcgatg ctcatcgagc aggctcgagc actagctcgc
841 actgggatga agagacacct ccgagttctc cccatgtatg caggactgcc ttcctttgag
901 caaatgaaag tgtttgaaag ggtgtcacgc agtgtcagaa aggtgatagt ggccaccaat
961 gtggcagaaa cctctatcac aatcagcggc attgtgtatg tgatcgactg tggctttgtg
1021 aaactccgag cctacaatcc caggacagct attgaatgct tggtggtggt gccagtctcc
1081 caggcatcag ctaatcagcg agcaggacgt ggtggtcgta gtcgctcggg aaaatgttat
1141 cgcctttata cagaggaagc ctttgacaag ttgcctcagt ctacggttcc tgagatgcag
1201 cgtagtaatt tggcacctgt catcctgcag ctgaaagcac taggaattga caatgtcctc
1261 aggttccact tcatgtcgcc ccctccagca cagtcgatgg ttcaagcctt ggagttactg
1321 tatgctctgg gaggtctgga caaagactgt cgcctaactg aaccgcttgg catgagaatt
1381 gcagagtttc ctttgaatcc catgtttgcc aaaatgctgc ttgaatcagg aaacttcggc
1441 tgttctcagg aaattctaag catcgctgcc atgatgcaga tccagaatat ctttgtggtc
1501 cccccaaacc agaagtctca cgcaattcga gtgcaccgta aatttgctgt ggaggagggc
1561 gaccacctca ctatgctcaa tatatatgaa gcatttatca aacacaataa ggactctaaa
1621 tggtgtcagg aacatttcct gaattacaag ggtcttgtca gagctgcgac tgtaagagaa
1681 caattgaaaa agcttcttgt caagtttcaa gtgcccagga agtctagtga aggtgacccg
1741 gatctggttc tgaggtgcat tgtctccggc ttcttcgcca atgcagcgag gtttcattct
1801 actggagctt ataggaccat ccgtgatgac catgagctgc acatacaccc tgcgtcagtc
1861 ctctatgcag agaagccgcc tcgctgggtc atctataacg aagttataca gacctccaag
1921 tactacatga gagatgtgac tgccattgaa tcggcctggc tgttggagct ggctccacac
1981 ttttatcaac aaggaacgca cctgtctctg aaagccaaaa gggccaaggt ccaggacccg
2041 tgagaggagc ccacagctac agctgcaggg actgctggcg tcctctcctc catgctgctg
2101 cccctggtcc caggtggggt gagctggcac cagctcctgt ggaatgtttg gttgctctga
2161 agtgggctgc ggcctgttca ttagtccttt cttcccgctg cccacagcat ttgcatcctt
2221 gctgggatcc tggaggactt tgtgcatggg caggcatcct tctgtgctgc agcgggcaga
2281 gtgggagttg gctcactcag cacgctcact aacccagcat gccacttcca gcaggcatgc
2341 agtcctg
//