LOCUS AK303340 3371 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ53442 complete cds, highly similar to Poly
(ADP-ribose) polymerase 1 (EC 2.4.2.30).
ACCESSION AK303340
VERSION AK303340.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3371)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3371
/clone="THYMU3007124"
/clone_lib="THYMU3"
/db_xref="H-InvDB:HIT000497964"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="thymus"
CDS 162..3143
/codon_start=1
/note="highly similar to Poly (ADP-ribose) polymerase 1
(EC 2.4.2.30)"
/protein_id="BAG64403.1"
/transl_table=1
/translation="MAESSDKLYRVEYAKSGRASCKKCSESIPKDSLRMAIMVQSPMF
DGKVPHWYHFSCFWKVGHSIRHPDVEVDGFSELRWDDQQKVKKTLGDFAAEYAKSNRS
TCKGCMEKIEKGQVRLSKKMVDPEKPQLGMIDRWYHPGCFVKNREELGFRPEYSASQL
KGFSLLATEDKEALKKQLPGVKSEGKRKGDEVDGVDEVAKKKSKKEKDKDSKLEKALK
AQNDLIWNIKDELKKVCSTNDLKELLIFNKQQVPSGESAILDRVADGMVFGALLPCEE
CSGQLVFKSDAYYCTGDVTAWTKCMVKTQTPNRKEWVTPKEFREISYLKKLKVKKQDR
IFPPETSASVAATPPPSTASAPAAVNSSASADKPLSNMKILTLGKLSRNKDEVKAMIE
KLGGKLTGTANKASLCISTKKEVEKMNKKMEEVKEANIRVVSEDFLQDVSASTKSLQE
LFLAHILSPWGAEVKAEPVEVVAPRGKSGAALSKKSKGQVKEEGINKSEKRMKLTLKG
GAAVDPDSGLEHSAHVLEKGGKVFSATLGLVDIVKGTNSYYKLQLLEDDKENRYWIFR
SWGRVGTVIGSNKLEQMPSKEDAIEHFMKLYEEKTGNAWHSKNFTKYPKKFYPLEIDY
GQDEEAVKKLTVNPGTKSKLPKPVQDLIKMIFDVESMKKAMVEYEIDLQKMPLGKLSK
RQIQAAYSILSEVQQAVSQGSSDSQILDLSNRFYTLIPHDFGMKKPPLLNNADSVQAK
VEMLDNLLDIEVAYSLLRGGSDDSSKDPIDVNYEKLKTDIKVVDRDSEEAEIIRNYVK
NTHATTHNAYDLEVIDIFKIEREGECQRYKPFKQLHNRRLLWHGSRTTNFAGILSQGL
RIAPPEAPVTGYMFGKGIYFADMVSKSANYCHTSQGDPIGLILLGEVALGNMYELKHA
SHISKLPKGKHSVKGLGKTTPDPSANISLDGVDVPLGTGISSGVNDTSLLYNEYIVYD
IAQVNLKYLLKLKFNFKTSLW"
BASE COUNT 903 a 790 c 958 g 720 t
ORIGIN
1 aatctatcag ggaacggcgg tggccggtgc ggcgtgttcg gtggcggctc tggccgctca
61 ggcgcctgcg gctgggtgag cgcacgcgag gcggcgaggc ggcagcgtgt ttctaggtcg
121 tggcgtcggg cttccggagc tttggcggca gctaggggag gatggcggag tcttcggata
181 agctctatcg agtcgagtac gccaagagcg ggcgcgcctc ttgcaagaaa tgcagcgaga
241 gcatccccaa ggactcgctc cggatggcca tcatggtgca gtcgcccatg tttgatggaa
301 aagtcccaca ctggtaccac ttctcctgct tctggaaggt gggccactcc atccggcacc
361 ctgacgttga ggtggatggg ttctctgagc ttcggtggga tgaccagcag aaagtcaaga
421 agactctggg tgactttgca gcagagtatg ccaagtccaa cagaagtacg tgcaaggggt
481 gtatggagaa gatagaaaag ggccaggtgc gcctgtccaa gaagatggtg gacccggaga
541 agccacagct aggcatgatt gaccgctggt accatccagg ctgctttgtc aagaacaggg
601 aggagctggg tttccggccc gagtacagtg cgagtcagct caagggcttc agcctccttg
661 ctacagagga taaagaagcc ctgaagaagc agctcccagg agtcaagagt gaaggaaaga
721 gaaaaggcga tgaggtggat ggagtggatg aagtggcgaa gaagaaatct aaaaaagaaa
781 aagacaagga tagtaagctt gaaaaagccc taaaggctca gaacgacctg atctggaaca
841 tcaaggacga gctaaagaaa gtgtgttcaa ctaatgacct gaaggagcta ctcatcttca
901 acaagcagca agtgccttct ggggagtcgg cgatcttgga ccgagtagcc gatggcatgg
961 tgttcggtgc cctccttccc tgcgaggaat gctcgggtca gctggtcttc aagagcgatg
1021 cctattactg cactggggac gtcactgcct ggaccaagtg tatggtcaag acacagacac
1081 ccaaccggaa ggagtgggta accccaaagg aattccgaga aatctcttac ctcaagaaat
1141 tgaaggttaa aaagcaggac cgtatattcc ccccagaaac cagcgcctcc gtggcggcca
1201 cgcctccgcc ctccacagcc tcggctcctg ctgctgtgaa ctcctctgct tcagcagata
1261 agccattatc caacatgaag atcctgactc tcgggaagct gtcccggaac aaggatgaag
1321 tgaaggccat gattgagaaa ctcgggggga agttgacggg gacggccaac aaggcttccc
1381 tgtgcatcag caccaaaaag gaggtggaaa agatgaataa gaagatggag gaagtaaagg
1441 aagccaacat ccgagttgtg tctgaggact tcctccagga cgtctccgcc tccaccaaga
1501 gccttcagga gttgttctta gcgcacatct tgtccccttg gggggcagag gtgaaggcag
1561 agcctgttga agttgtggcc ccaagaggga agtcaggggc tgcgctctcc aaaaaaagca
1621 agggccaggt caaggaggaa ggtatcaaca aatctgaaaa gagaatgaaa ttaactctta
1681 aaggaggagc agctgtggat cctgattctg gactggaaca ctctgcgcat gtcctggaga
1741 aaggtgggaa ggtcttcagt gccacccttg gcctggtgga catcgttaaa ggaaccaact
1801 cctactacaa gctgcagctt ctggaggacg acaaggaaaa caggtattgg atattcaggt
1861 cctggggccg tgtgggtacg gtgatcggta gcaacaaact ggaacagatg ccgtccaagg
1921 aggatgccat tgagcacttc atgaaattat atgaagaaaa aaccgggaac gcttggcact
1981 ccaaaaattt cacgaagtat cccaaaaagt tctaccccct ggagattgac tatggccagg
2041 atgaagaggc agtgaagaag ctgacagtaa atcctggcac caagtccaag ctccccaagc
2101 cagttcagga cctcatcaag atgatctttg atgtggaaag tatgaagaaa gccatggtgg
2161 agtatgagat cgaccttcag aagatgccct tggggaagct gagcaaaagg cagatccagg
2221 ccgcatactc catcctcagt gaggtccagc aggcggtgtc tcagggcagc agcgactctc
2281 agatcctgga tctctcaaat cgcttttaca ccctgatccc ccacgacttt gggatgaaga
2341 agcctccgct cctgaacaat gcagacagtg tgcaggccaa ggtggaaatg cttgacaacc
2401 tgctggacat cgaggtggcc tacagtctgc tcaggggagg gtctgatgat agcagcaagg
2461 atcccatcga tgtcaactat gagaagctca aaactgacat taaggtggtt gacagagatt
2521 ctgaagaagc cgagatcatc aggaattatg ttaagaacac tcatgcaacc acacacaatg
2581 cgtatgactt ggaagtcatc gatatcttta agatagagcg tgaaggcgaa tgccagcgtt
2641 acaagccctt taagcagctt cataaccgaa gattgctgtg gcacgggtcc aggaccacca
2701 actttgctgg gatcctgtcc cagggtcttc ggatagcccc gcctgaagcg cccgtgacag
2761 gctacatgtt tggtaaaggg atctatttcg ctgacatggt ctccaagagt gccaactact
2821 gccatacgtc tcagggagac ccaataggct taatcctgtt gggagaagtt gcccttggaa
2881 acatgtatga actgaagcac gcttcacata tcagcaagtt acccaagggc aagcacagtg
2941 tcaaaggttt gggcaaaact acccctgatc cttcagctaa cattagtctg gatggtgtag
3001 acgttcctct tgggaccggg atttcatctg gtgtgaatga cacctctcta ctatataacg
3061 agtacattgt ctatgatatt gctcaggtaa atctgaagta tctgctgaaa ctgaaattca
3121 attttaagac ctccctgtgg taattgggag aggtagccga gtcacacccg gtggctctgg
3181 tatgaattca cccgaagcgc ttctgcacca actcacctgg ccgctaagtt gctgatgggt
3241 agtacctgta ctaaaccacc tcagaaagga ttttacagaa acgtgttaaa ggttttctct
3301 aacttctcaa gtcccttgtt ttgtgttgtg tctgtgggga ggggttgttt tggggttgtt
3361 tttgtttttt t
//