LOCUS AK303020 1401 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ52814 complete cds, highly similar to
tRNA(5-methylaminomethyl-2-thiouridylate)-methyltransferase (EC
2.1.1.61).
ACCESSION AK303020
VERSION AK303020.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1401)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1401
/clone="TESTI4049538"
/clone_lib="TESTI4"
/db_xref="H-InvDB:HIT000497644"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="testis"
CDS 209..604
/codon_start=1
/note="highly similar to
tRNA(5-methylaminomethyl-2-thiouridylate)-
methyltransferase (EC 2.1.1.61)"
/protein_id="BAG64147.1"
/transl_table=1
/translation="MKNWDSLDEHGVCTADKDCEDAYRVCQILDIPFHQVSYVKEYWN
DVFSDFLNEYEKGRTPNPDIVCNKHIKFSCFFHYAVDNLGADAIATGHYARTSLEDEE
VFEQKHVKKPEGLFRNRFEVRNARFPRMP"
BASE COUNT 328 a 325 c 399 g 349 t
ORIGIN
1 gtgcacgtct cctccctccc tgggccgctg gttgcgcgcg ggtcggcagg aggatacccc
61 gtcctctgac tttggttcgg aggctcctcg ccctccacct gtgtagtcgg aggtgtgcgc
121 gactgcagct ccgactacct gggagcagtt ccgcgcccct ctccacccac gcgcgcccac
181 ccacagttac caggtgacag gggtgtttat gaagaactgg gactcactgg atgaacatgg
241 ggtctgtact gccgacaaag actgtgaaga tgcttacaga gtttgccaga tcttagacat
301 ccctttccat caagtgtcct acgtaaagga gtattggaat gatgtgttca gtgacttttt
361 gaatgagtat gaaaaaggaa ggactcccaa tcctgacata gtttgcaaca agcacatcaa
421 atttagttgc ttttttcatt atgctgtgga taatcttggg gcagatgcca ttgccacagg
481 tcactatgca agaacttccc tggaagatga agaagtcttt gagcagaagc acgttaagaa
541 gcccgaaggg cttttcagaa atcggtttga agttagaaat gccaggtttc ccaggatgcc
601 ctgaggagaa ccatcttccc tctgggggga ttaacgaaag agtttgtaaa gaaaatcgct
661 gctgagaata gacttcatca tgtgcttcag aagaaagaga gcatgggcat gtgtttcatc
721 gggaagagga attttgaaca tttccttctt cagtatctgc agcctcgacc tggtcacttt
781 atttccatag aagacaataa ggttctggga acacataaag gtgaggtgca gactctgcca
841 cttgtcatct gaaatgccta gagctgtggc gtttcagctc tgggagacta gaccagagtt
901 cctgtcgtcc cttccacttg gctggagaat tgtagaaggc tctgtggggc ggccgggggc
961 gggcacggcc tagggtggaa cagttgcctt gatggtggct gggtgcactt ccagatgtgg
1021 cctcagctga gatgctggag ttcatgaaaa gcgcgcctct gacttggaag gactatactc
1081 tgtgagttac gtatcaagtc cctgtaaaaa gaatgatttc cttcctgtgt aaggaaaatg
1141 ttaaactaca caaaactcca ctctgattca gggagtaagg tggacgtccc ggcatggtgt
1201 gaatgattaa ggaccacatg cagcgatggg atcaggcgtc cgcgctggcc agagtgctgc
1261 tcgctcatgt gcagaccctt actgcggtga ggcctgcgtg agcctgtgct gtgcctggtg
1321 tcaggggaca ggctccgagt cagtcacagt gtgcagtcct gctgtgggga cagagtgcag
1381 ccattgttgg gttagtacag t
//