LOCUS AK302951 3024 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ58309 complete cds, highly similar to
Mitogen-activated protein kinase kinasekinase 13 (EC 2.7.11.25).
ACCESSION AK302951
VERSION AK302951.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3024)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3024
/clone="TESTI4044165"
/clone_lib="TESTI4"
/db_xref="H-InvDB:HIT000497575"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="testis"
CDS 201..2669
/codon_start=1
/note="highly similar to Mitogen-activated protein kinase
kinasekinase 13 (EC 2.7.11.25)"
/protein_id="BAG64106.1"
/transl_table=1
/translation="MSYVECKCLQLENKNTWEVPFEEISELQWLGSGAQGAVFLGKFR
AEEVAIKKVREQNETDIKHLRKLKHPNIIAFKGVCTQAPCYCIIMEYCAHGQLYEVLR
AGRKITPRLLVDWSTGIASGMNYLHLHKIIHRDLKSPNVLVTHTDAVKISDFGTSKEL
SDKSTKMSFAGTVAWMAPEVIRNEPVSEKVDIWSFGVVLWELLTGEIPYKDVDSSAII
WGVGSNSLHLPVPSTCPDGFKILMKQTWQSKPRNRPSFRQTLMHLDIASADVLATPQE
TYFKSQAEWREEVKKHFEKIKSEGTCIHRLDEELIRRRREELRHALDIREHYERKLER
ANNLYMELSAIMLQLEMREKELIKREQAVGKKYPGTYKRHPVRPIIHPNAMEKLMKRK
GVPHKSGMQTKRPDLLRSEGIPTTEVAPTASPLSGSPKMSTSSSKSRYRSKPRHRRGN
SRGSHSDFAAILKNQPAQENSPHPTYLHQAQSQYPSLHHHNSLQQQYQQPPPAMSQSH
HPRLNMHGQDIATCANNLRYFGPAAALRSPLSNHAQRQLPGSSPDLISTAMAADCWRS
SEPDKGQAGPWGCCQADAYDPCLQCRPEQYGSLDIPSAEPVGRSPDLSKSPAHNPLLE
NAQSSEKTEENEFSGCRSESSLGTSHLGTPPALPRKTRPLQKSGDDSSEEEEGEVDSE
VEFPRRQRPHRCISSCQSYSTFSSENFSVSDGEEGNTSDHSNSPDELADKLEDRLAEK
LDDLLSQTPEIPIDISSHSDGLSDKECAVRRVKTQMSLGKLCVEERGYENPMQFEESD
CDSSDGECSDATVRTNKHYSSATW"
BASE COUNT 871 a 728 c 719 g 706 t
ORIGIN
1 agtttagtct gtgaggaaac tggcggctgc tggtagttcc atgctgtctg ttcagacttc
61 agcttggtgg attagaacta ctgaaaagaa agtttcagca ctcagcagta gttttatttt
121 ttttaatgga aaagaaagcc gtgaggtgta tcagcaagtg tgctgctaaa acaggtcccg
181 cgtgcacgaa atgatttcta atgtcttatg ttgagtgcaa gtgtttacag ttagaaaata
241 aaaatacttg ggaagtgcca tttgaggaga tctcagagct gcagtggctg ggtagtggag
301 cccaaggagc ggtcttcttg ggcaagttcc gggcggaaga ggtggccatc aagaaagtga
361 gagaacagaa tgagacggat atcaagcatt tgaggaagtt gaagcaccct aacatcatcg
421 cattcaaggg tgtttgtact caggccccat gttattgtat tatcatggaa tactgtgccc
481 atggacaact ctacgaggtc ttacgagctg gcaggaagat cacacctcga ttgctagtag
541 actggtccac aggaattgca agtggaatga attatttgca cctccataaa attattcatc
601 gtgatctcaa atcacctaat gttttagtga cccacacaga tgcggtaaaa atttcagatt
661 ttggtacatc taaggaactc agtgacaaaa gtaccaagat gtcatttgct ggcacggtcg
721 catggatggc gccagaggtg atacggaatg aacctgtctc tgaaaaagtt gatatatggt
781 cttttggagt ggtgctttgg gagctgctga caggagagat cccttacaaa gatgtagatt
841 cttcagccat tatctggggt gttggaagca acagcctcca ccttccagtt ccttccactt
901 gccctgatgg attcaaaatc cttatgaaac agacgtggca gagtaaacct cgaaaccgac
961 cttcttttcg gcagacactc atgcatttag acattgcctc tgcagatgta cttgccaccc
1021 cacaagaaac ttacttcaag tctcaggctg aatggagaga agaagtgaaa aaacattttg
1081 agaagatcaa aagtgaagga acttgtatac accggttaga tgaagaactg attcgaaggc
1141 gcagagaaga gctcaggcat gcgctggata ttcgtgaaca ctatgagcgg aagcttgagc
1201 gggcgaataa tttatacatg gaattgagtg ccatcatgct gcagctagaa atgcgggaga
1261 aggagctcat taagcgtgag caagcagtgg gaaagaagta tcctgggacc tacaaacgac
1321 accctgttcg tcctatcatc catcccaatg ccatggagaa actcatgaaa aggaaaggag
1381 tgcctcacaa atctgggatg cagaccaaac ggccagactt gttgagatca gaagggatcc
1441 ccaccacaga agtggctccc actgcatccc ctttgtccgg aagtcccaaa atgtccactt
1501 ctagcagcaa gagccgatat cgaagcaaac cacgccaccg ccgagggaat agcagaggca
1561 gccatagtga ctttgccgca atcttgaaaa accagccagc ccaggaaaat tcaccccatc
1621 ccacttacct gcaccaagct caatcccaat acccttctct tcatcaccat aattctctgc
1681 agcagcaata ccagcagccc cctcctgcca tgtcccagag tcaccatccc agactcaata
1741 tgcacggaca ggacatagca acctgcgcca acaacctgag gtatttcggc ccagcagcag
1801 ccctgcggag cccactcagc aaccatgctc agagacagct gcccggctcg agccctgacc
1861 tcatctccac agccatggct gcagactgct ggagaagttc tgagcctgac aagggccaag
1921 ctggtccctg gggctgttgc caggctgacg cttatgaccc ctgccttcag tgcaggccag
1981 aacagtatgg gtccttagac ataccctctg ctgagccagt ggggaggagc cctgaccttt
2041 ccaagtcacc agcacataat cctctcttgg aaaacgccca gagttctgag aaaacggaag
2101 aaaatgaatt cagcggctgt aggtctgagt catccctcgg cacctctcat ctcggcaccc
2161 ctccagcgct acctcgaaaa acaaggcctc tgcagaagag tggagatgac tcctcagaag
2221 aggaagaagg ggaagtagat agtgaagttg aatttccacg aagacagagg ccccatcgct
2281 gtatcagcag ctgccagtca tattcaacct ttagctctga gaatttctct gtgtctgatg
2341 gagaagaggg aaataccagt gaccactcaa acagtcctga tgagttagct gataaacttg
2401 aagaccgctt ggcagagaag ctagacgacc tgctgtccca gacgccagag attcccattg
2461 acatatcctc acactcggat gggctctctg acaaggagtg tgccgtgcgc cgtgtgaaga
2521 ctcagatgtc tctgggcaag ctgtgtgtgg aggaacgtgg ctatgagaac cccatgcagt
2581 ttgaagaatc ggactgtgac tcttcagatg gggagtgttc tgatgccaca gttaggacca
2641 ataaacacta cagctctgct acctggtaat gaaggaatac acatcctgaa gatctcgtga
2701 ctatactggc atttcagatc caccccaccc ccagactcat cccactctct cccagcattt
2761 tgtctgggaa gagagactac cccatcttta ccacccccta gaaatgagct gcaataacag
2821 gaacatgaga cttcgcaaat ctctggaaaa taatatccaa atgaaattaa gtctcactga
2881 acatttcaat caagaatggc agggatctat tttattgaat attctagcta ctgtaacatt
2941 gatatttatt tttgtttgac attttaacac tttgtactgc aaagagtgaa ctatatatga
3001 gatagagaga caataatttc ttgc
//