LOCUS AK302877 2888 bp mRNA linear HUM 31-JUL-2008
DEFINITION Homo sapiens cDNA FLJ58806 complete cds, highly similar to
DNA-directed RNA polymerase, mitochondrial precursor (EC 2.7.7.6).
ACCESSION AK302877
VERSION AK302877.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2888)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2888
/clone="TESTI4037782"
/clone_lib="TESTI4"
/db_xref="H-InvDB:HIT000497501"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="testis"
CDS 56..2839
/codon_start=1
/note="highly similar to DNA-directed RNA polymerase,
mitochondrial precursor (EC 2.7.7.6)"
/protein_id="BAG64057.1"
/transl_table=1
/translation="MSALCWGRGAAGLKRALRPCGRPGLPGKEGTAGGVCGPRRSSSA
SPQEQDQDRRKDWGHVELLEVLQARVRQLQAESVSEVVVNRVDVARLPECGSGDGSLQ
PPRKVQMGAKDATPVPCGRWAKILEKDKRTQQMRMQRLKAKLQMPFQNGEFKALTRRL
QVEPRLLSKQMAGCLEDCTRQAPESPWEEQLARLLQEAPGKLSLDVEQAPSGQHSQAQ
LSGQQQRLLAFFKCCLLTDQLPLAHHLLVVHHGQRQKRKLLTLDMYNAVMLGWARQGA
FKELVYVLFMVKDAGLTPDLLSYAAALQCMGRQDQDAGTIERCLEQMSQEGLKLQALF
TAVLLSEEDRATVLKAVHKVKPTFSLPPQLPPPVNTSKLLRDVYAKDGRVSYPKLHLP
LKTLQCLFEKQLHMELASRLCVVSVEKPTLPSKEVKHARKTLKTLRDQWEKALCRALR
ETKNRLEREVYEGRFSLYPFLCLLDEREVVRMLLQVLQALPAQGESFTTLARELSART
FSRHVVQRQRVSGQVQALQNHYRKYLCLLASDAEVPEPCLPRQYWEALGAPEALREQP
WPLPVQMELGKLLAEMLVQATQMPCSLDKPHRSSRLAPVLYHVYSFRNVQQIGILKPH
PAYVQLLEKAAEPTLTFEAVDVPMLCPPLPWTSPHSGAFLLSPTKLMRTVEGATQHQE
LLETCPPTALHGALDALTQLGNCAWRVNGRVLDLVLQLFQAKGCPQLGVPAPPSEAPQ
PPEAHLPHSAAPARKAELRRELAHCQKVAREMHSLRAEALYRLSLAQHLRDRVFWLPH
NMDFRGRTYPCPPHFIHSLDSSHMMLTALHCYRSPPLCHRKGLTFVSVHDCYWTHAAD
VSVMNQVCREQFVRLHSEPILQDLSRFLVKRFCSEPQKILEASQLKETLQAVPKPGAF
DLEQVKRSTYFFS"
BASE COUNT 495 a 960 c 972 g 461 t
ORIGIN
1 gcgggccggt ggggtggcgg cggcggcggc ggcggcgcct ggagcggcgt gcgtaatgtc
61 ggcactttgc tggggccgcg gagcggcggg gctcaaacga gccctacggc cttgcggccg
121 cccgggactc cccggcaaag aagggaccgc cggtggcgtc tgcggcccca ggaggagctc
181 gtccgccagc ccccaggagc aagaccaaga ccgcaggaag gactggggcc acgtggagct
241 gctggaggtg ctccaggcgc gggtgcggca gctgcaggct gagagcgtgt cggaggtggt
301 ggtgaacagg gtggatgtgg cgcggctccc agaatgtggc agtggagatg gtagcctcca
361 gccacccagg aaggtccaga tgggggccaa ggatgccacc ccggtgccct gtggccgctg
421 ggcaaagata ctggagaagg ataagcggac ccagcagatg cgtatgcagc ggttgaaggc
481 gaagctgcag atgccattcc agaacgggga gttcaaggcg ctgaccaggc gcctgcaggt
541 ggagccccgg ctcctgagca agcagatggc tgggtgcctg gaggactgca cgcgccaggc
601 ccccgagagc ccctgggagg agcagctggc ccggctgctg caggaggccc ctgggaagct
661 gagcctcgat gtggagcagg ccccgtcggg gcagcactcg caggcccagc tctcaggtca
721 gcagcagagg ctcctggcct tcttcaagtg ctgcctgctc actgaccagc tgcccctcgc
781 ccaccacctg ctggtcgtcc accacggcca gcggcagaag cggaagctgc tcacgctgga
841 catgtacaac gccgtgatgc ttggctgggc gcggcagggt gccttcaagg agctggtata
901 tgtgttattc atggtgaagg atgccggctt gaccccggac ctgctgtcct atgcggctgc
961 cctccagtgc atggggaggc aggaccagga cgccgggacc atcgaaaggt gtctggaaca
1021 gatgagccag gaggggctga agctgcaggc actcttcacc gccgttctgc tgtctgagga
1081 ggatcgggcc actgttctga aggccgtgca caaggtgaag cccaccttca gcctcccgcc
1141 gcagctgccg cccccggtca acacctccaa gctgctcagg gacgtgtatg ccaaggatgg
1201 gcgtgtgtcc tacccgaagc tgcacctgcc cttgaagacc ctgcagtgcc tctttgagaa
1261 gcagctccac atggagctgg ccagcaggtt gtgcgtggtg tccgtggaga agcccacgtt
1321 gccaagcaag gaggtcaagc acgcgcggaa gaccctgaag accctgcggg accaatggga
1381 gaaagcactg tgccgggcgc tgcgggagac caagaaccgc ctagagcgcg aggtgtacga
1441 gggccggttc tcactttacc ccttcctgtg cctgctggac gagcgcgagg tggtgcggat
1501 gctcctgcag gtcctgcagg cgctgcccgc ccaaggtgag tccttcacca ccctggcccg
1561 ggagctgagt gcgcgcactt tcagccggca cgtggtgcag aggcagcggg tcagtggcca
1621 ggtgcaggcg ctgcagaacc actacaggaa gtacctctgc ttgctggcct ccgacgccga
1681 ggtgcccgag ccctgcctgc cgcggcagta ctgggaggcg ctgggggcgc ccgaggccct
1741 gcgggagcag ccctggcccc tgccagtgca gatggagctg ggcaagctgc tggcggagat
1801 gctggtgcag gctacgcaga tgccatgcag cctggacaag ccgcatcgtt cctctcggct
1861 tgcccccgtg ctctaccacg tgtattcctt ccgcaacgtc cagcagatcg gcatcctgaa
1921 gccgcacccg gcctacgtgc agctgctgga gaaggccgcg gagcccacgc tgaccttcga
1981 ggcggtggat gtacccatgc tttgcccccc gctgccctgg acatcgccgc actctggtgc
2041 cttcctgctc agccccacca agctgatgcg cacggtggaa ggcgccacgc agcaccagga
2101 gctgctggaa acctgcccgc ccaccgcgct gcatggcgca ctggacgccc tcacccaact
2161 gggcaactgc gcctggcgcg tcaacgggcg cgtgctggac ctggtgctgc agctcttcca
2221 ggccaagggc tgcccccagc taggcgtgcc ggccccgccc tccgaggcgc cccagccgcc
2281 cgaggcccac ctgccgcaca gcgccgcgcc cgcccgcaag gccgagctgc gccgtgagct
2341 ggcgcactgc cagaaggtgg cccgggagat gcacagcctg cgggcggagg cgctgtaccg
2401 cctctcgctg gcgcagcacc tgcgggaccg cgtcttctgg ctgccgcaca acatggactt
2461 ccgcggccgc acctacccct gcccgccgca cttcatccac tcgctggact cctcccacat
2521 gatgctcacc gccctgcact gctacaggag tcctccgctc tgccacagga agggcctgac
2581 cttcgtctct gtgcacgact gttactggac tcacgcagct gatgtctccg tcatgaacca
2641 ggtgtgccgg gagcagtttg tccgcttgca cagcgagccc attctgcagg acctgtccag
2701 attcctggtc aagcggttct gctctgagcc ccagaagatc ttggaggcca gccagctgaa
2761 ggagacactg caggcggtgc ccaagccagg ggccttcgac ctggagcagg tgaagcgttc
2821 cacctacttc ttcagctgac accccgtgag ccttgtcagt gtgtaaataa agctcttttg
2881 ccaccccc
//