LOCUS AK302683 2449 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ53869 complete cds, highly similar to
Galactocerebrosidase precursor (EC 3.2.1.46).
ACCESSION AK302683
VERSION AK302683.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2449)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2449
/clone="TESTI4020777"
/clone_lib="TESTI4"
/db_xref="H-InvDB:HIT000497307"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="testis"
CDS 430..2199
/codon_start=1
/note="highly similar to Galactocerebrosidase precursor
(EC 3.2.1.46)"
/protein_id="BAH13778.1"
/transl_table=1
/translation="MGFMVADLWATSRLLVNYPEPHRSQILDYLFKPNFGASLHILKV
EIGGDGQTTDGTEPSHMHYALDENYFRGYEWWLMKEAKKRNPNITLIGLPWSFPGWLG
KGFDWPYVNLQLTAYYVVTWIVGAKRYHDLDIDYIGIWNERSYNANYIKILRKMLNYQ
GLQRVKIIASDNLWESISASMLLDAELFKVVDVIGAHYPGTHSAKDAKLTGKKLWSSE
DFSTLNSDMGAGCWGRILNQNYINGYMTSTIAWNLVASYYEQLPYGRCGLMTAQEPWS
GHYVVESPVWVSAHTTQFTQPGWYYLKTVGHLEKGGSYVALTDGLGNLTIIIETMSHK
HSKCIRPFLPYFNVSQQFATFVLKGSFSEIPELQVWYTKLGKTSERFLFKQLDSLWLL
DSDGSFTLSLHEDELFTLTTLTTGRKGSYPLPPKSQPFPSTYKDDFNVDYPFFSEAPN
FADQTGVFEYFTNIEDPGEHHFTLRQVLNQRPITWAADASNTISIIGDYNWTNLTIKC
DVYIETPDTGGVFIAGRVNKGGILIRSARGIFFWIFANGSYRVTGDLAGWIIYALGRV
EVTAKKWYTLTLTIKVAGRRKKT"
BASE COUNT 659 a 500 c 609 g 681 t
ORIGIN
1 taggtttgaa caaatgagga tccggaggtc cagagagtat aggtgcagag agtaaagtga
61 caggtctaag tagtgaagta gccggggctg gaaccctcgt cccaggcagt ggggcacagg
121 ctgtcttcat cgcggagatg gcagtcgggg agcgtcgctc cagaaagggc cagggagtgc
181 gggagcgtcc gtgtgcgaaa gagaacgccc cagtgggatg ctttggctgt gtctctccct
241 tttggaggag ttggtgggtg gtggagtcgt taagtcccgt gcagttgtta gtgcagggta
301 agaatgggct cctccagaga gggtagcgcc gaataggatt tcgtttggag gcaggactgg
361 tgcctcaagg tctctttaaa aggaagataa gcgagcggcc tgaaactacc gggtactccc
421 tctgggccca tgggcttcat ggtggccgac ctttgggcaa cctcccgact tctagtaaat
481 tacccagagc cccatcgttc tcagatattg gattatctct ttaagccgaa ttttggtgcc
541 tctttgcata ttttaaaagt ggaaataggt ggtgatgggc agacaacaga cggcactgag
601 ccctcccaca tgcattatgc actagatgag aattatttcc gaggatacga gtggtggttg
661 atgaaagaag ctaagaagag gaatcccaat attacactca ttgggttgcc atggtcattc
721 cctggatggc tgggaaaagg tttcgactgg ccttatgtca atcttcagct gactgcctat
781 tatgtcgtga cctggattgt gggcgccaag cgttaccatg atttggacat tgattatatt
841 ggaatttgga atgagaggtc atataatgcc aattatatta agatattaag aaaaatgctg
901 aattatcaag gtctccagcg agtgaaaatc atagcaagtg ataatctctg ggagtccatc
961 tctgcatcca tgctccttga tgccgaactc ttcaaggtgg ttgatgttat aggggctcat
1021 tatcctggaa cccattcagc aaaagatgca aagttgactg ggaagaagct ttggtcttct
1081 gaagacttta gcactttaaa tagtgacatg ggtgcaggct gctggggtcg cattttaaat
1141 cagaattata tcaatggcta tatgacttcc acaatcgcat ggaatttagt ggctagttac
1201 tatgaacagt tgccttatgg gagatgcggg ttgatgacgg cccaagagcc atggagtggg
1261 cactacgtgg tagaatctcc tgtctgggta tcagctcata ccactcagtt tactcaacct
1321 ggctggtatt acctgaagac agttggccat ttagagaaag gaggaagcta cgtagctctg
1381 actgatggct tagggaacct caccatcatc attgaaacca tgagtcataa acattctaag
1441 tgcatacggc catttcttcc ttatttcaat gtgtcacaac aatttgccac ctttgttctt
1501 aagggatctt ttagtgaaat accagagcta caggtatggt ataccaaact tggaaaaaca
1561 tccgaaagat ttctttttaa gcagctggat tctctatggc tccttgacag cgatggcagt
1621 ttcacactga gcctgcatga agatgagctg ttcacactca ccactctcac cactggtcgc
1681 aaaggcagct acccgcttcc tccaaaatcc cagcccttcc caagtaccta taaggatgat
1741 ttcaatgttg attacccatt ttttagtgaa gctccaaact ttgctgatca aactggtgta
1801 tttgaatatt ttacaaatat tgaagaccct ggcgagcatc acttcacgct acgccaagtt
1861 ctcaaccaga gacccattac gtgggctgcc gatgcatcca acacaatcag tattatagga
1921 gactacaact ggaccaatct gactataaag tgtgatgttt acatagagac ccctgacaca
1981 ggaggtgtgt tcattgcagg aagagtaaat aaaggtggta ttttgattag aagtgccaga
2041 ggaattttct tctggatttt tgcaaatgga tcttacaggg ttacaggtga tttagctgga
2101 tggattatat atgctttagg acgtgttgaa gttacagcaa aaaaatggta tacactcacg
2161 ttaactatta aggtggctgg aagaaggaag aaaacctgat atagccaatg ctgttgtagt
2221 cgtttcctca gcctcatctc actgtgctgt ggtctgtcct cacatgtgca ctggtaacag
2281 actcacacag ctgatgaatg cttttctctc cttatgtgtg gaaggagggg agcacttaga
2341 catttgctaa ctcccagaat tggatcatct cctaagatgt acttactttt taaagtccaa
2401 atatgtttat atttaaatat acgtgagcat gttcatcatg ttgtatgat
//