LOCUS AK302578 2056 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ53247 complete cds, highly similar to
Insulin-degrading enzyme (EC 3.4.24.56).
ACCESSION AK302578
VERSION AK302578.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2056)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2056
/clone="TESTI4013916"
/clone_lib="TESTI4"
/db_xref="H-InvDB:HIT000497202"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="testis"
CDS 279..1673
/codon_start=1
/note="highly similar to Insulin-degrading enzyme (EC
3.4.24.56)"
/protein_id="BAH13752.1"
/transl_table=1
/translation="MSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLEL
LKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIEKMATFEIDEK
RFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAF
IPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPD
RGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGY
IVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQAL
AIRRLDKPKKLSAECAEYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFYKEMLAVD
APRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLF
PLVKPHINFMAAKL"
BASE COUNT 667 a 431 c 399 g 559 t
ORIGIN
1 gataatattg ccagctgcca gaccagacta gggtggctca aggggttgcc atagtttcta
61 aatcttttga aggaaaaact gatcgcacag aagagtggta tggaacccag tacaaacaag
121 aagctatacc ggatgaagtc atcaagaaat ggcaaaatgc tgacctgaat gggaaattta
181 aacttcctac aaagaatgaa tttattccta cgaattttga gattttaccg ttagaaaaag
241 aggcgacacc ataccctgct cttattaagg atacagctat gagcaaactt tggttcaaac
301 aagatgataa gttttttttg ccgaaggctt gtctcaactt tgaatttttc agcccatttg
361 cttatgtgga ccccttgcac tgtaacatgg cctatttgta ccttgagctc ctcaaagact
421 cactcaacga gtatgcatat gcagcagagc tagcaggctt gagctatgat ctccaaaata
481 ccatctatgg gatgtatctt tcagtgaaag gttacaatga caagcagcca attttactaa
541 agaagattat tgagaaaatg gctacctttg agattgatga aaaaagattt gaaattatca
601 aagaagcata tatgcgatct cttaacaatt tccgggctga acagcctcac cagcatgcca
661 tgtactacct ccgcttgctg atgactgaag tggcctggac taaagatgag ttaaaagaag
721 ctctggatga tgtaaccctt cctcgcctta aggccttcat acctcagctc ctgtcacggc
781 tgcacattga agcccttctc catggaaaca taacaaagca ggctgcatta ggaattatgc
841 agatggttga agacaccctc attgaacatg ctcataccaa acctctcctt ccaagtcagc
901 tggttcggta tagagaagtt cagctccctg acagaggatg gtttgtttat cagcagagaa
961 atgaagttca caataactgt ggcatcgaga tatactacca aacagacatg caaagcacct
1021 cagagaatat gtttctggag ctcttctgtc agattatctc ggaaccttgc ttcaacaccc
1081 tgcgcaccaa ggagcagttg ggctatatcg tcttcagcgg gccacgtcga gctaatggca
1141 tacagggctt gagattcatc atccagtcag aaaagccacc tcactaccta gaaagcagag
1201 tggaagcttt cttaattacc atggaaaagt ccatagagga catgacagaa gaggccttcc
1261 aaaaacacat tcaggcatta gcaattcgtc gactagacaa accaaagaag ctatctgctg
1321 agtgtgctga atactgggga gaaatcatct cccagcaata taattttgac agagataaca
1381 ctgaggttgc atatttaaag acacttacca aggaagatat catcaaattc tacaaggaaa
1441 tgttggcagt agatgctcca aggagacata aggtatccgt ccatgttctt gccagggaaa
1501 tggattcttg tcctgttgtt ggagagttcc catgtcaaaa tgacataaat ttgtcacaag
1561 caccagcctt gccacaacct gaagtgattc agaacatgac cgaattcaag cgtggtctgc
1621 cactgtttcc ccttgtgaaa ccacatatta acttcatggc tgcaaaactc tgaagattcc
1681 ccatgcatgg gaaagtgcaa gtggatgcat tcctgagtct tccagagcct aagaaaatca
1741 tcttggccac tttaatagtt tctgattcac tattagagaa acaaacaaaa aattgtcaaa
1801 tgtcattatg tagaaatatt ataaatccaa agtaaattac aaaatcttat agatgtagaa
1861 tattttttaa atacatgcct cttaaatatt ttaaaatttt tcttttgatt actgagagaa
1921 atttccccaa tataacaatg cttaaaatga atgatattcc tatagaatct tccttcccta
1981 ttctgtaaaa tagtcacttg tccgaagaaa gttaaaagtt agctcttttc taaaagcctc
2041 ctagcttgac atagaa
//