LOCUS AK302543 6763 bp mRNA linear HUM 31-JUL-2008
DEFINITION Homo sapiens cDNA FLJ60974 complete cds, highly similar to
Mediator of RNA polymerase II transcription subunit 12.
ACCESSION AK302543
VERSION AK302543.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 6763)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..6763
/clone="TESTI4011964"
/clone_lib="TESTI4"
/db_xref="H-InvDB:HIT000497167"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="testis"
CDS 200..6202
/codon_start=1
/note="highly similar to Mediator of RNA polymerase II
transcription subunit 12"
/protein_id="BAG63815.1"
/transl_table=1
/translation="MAAFGILSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQG
FNNQPAVSGDEHGSAKNVSFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNF
WLVTARSQSAINTWFTDLAGTKPLTQLAKKVSIFSKKEEVFGYLAKYTVPVMRAAWLI
KMTCAYYAAISETKVKKRHVDPFMEWTQIITKYLWEQLQKMAEYYRPGPAGSGGCGST
IGPLPHDVEVAIRQWDYTEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLKLL
LPLLLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGVSSHSSHVISAQSTSTLPTTPAP
QPPTSSTPSTPFSDLLMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTGS
PLDHLPIAPSNLPMPEGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQEATAG
FTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGPSKDGHEISSDDDAVVSLLC
EWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSIASGSLSAPSAPIF
QDVLLQFLDTQAPMLTDPRSESERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGDL
AFGAPGPRPPSPFDDPADDPEHKEAEGSSSSKLEDPGLSESMDIDPSSSVLFEDMEKP
DFSLFSPTMPCEGKGSPSPEKPDVEKEVKPPPKEKIEGTLGVLYDQPRHVQYATHFPI
PQEESCSHECNQRLVVLFGVGKQRDDARHAIKKITKDILKVLNRKGTAETDQLAPIVP
LNPGDLTFLGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHYDQHQVTAQVSRNVLEQI
TSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLLKSSDL
VGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCIL
AYLYDLYTSCSHLKNKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENPA
AHTFTYTGLGKSLSENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSL
SAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDL
IRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQSDGNKPTVGIRSSCD
RHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVTGGTEELPEEEGGGGSGGRR
QGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRL
MQLICYPHRLLDNEDGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTPNNEMNSL
LENIAKATIEVFQQSAETGSSSGSTASNMPSSSKTKPVLSSLERSGVWLVAPLIAKLP
TSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDE
QREGLLTSLYSQMHQIVNNWRDDQYLDDCKPKQLMHEALKLRLNLVGGMFDTVQRSTQ
QTTEWAMLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENK
RAYMNLAKKLQKELGERQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGF
DSIFKKEGLQVSTKQKISPWDLFEGLKPSAPLSWGWFGTVRVDRRVARGEEQQRLLLY
HTHLRPRPRAYYLEPLPLPPEDEEPPAPTLLEPEKKAPEPPKTDKPGAAPPSTEERKK
KSTKGKKRSQPATKTEDYGMGPGRSGPYGVTVPPDLLHHPNPGSITHLNYRQGSIGLY
TQNQPLPAGGPRVDPYRPVRLPMQKLPTRPTYPGVLPTTMTGVMGLEPSSYKTSVYRQ
QQPAVPQGQRLRQQLQQSQGMLGQSSVHQMTPSSSYGLQTSQGYTPYVSHVGLQQHTG
PADPTRHLQQRPSGYVHQQAPTYGHGLTSTQRFHTRHCSRHP"
BASE COUNT 1580 a 1961 c 1760 g 1462 t
ORIGIN
1 attgtccgat ggttcccggc gtacctcggc ttccctcggt agtttccggc aatggtcgag
61 agtttctaac gtgccccctt gttgtctctc ggccgccgtc ctctcaacca ccgcccccct
121 tttcggctcc ctctccccct tcccgttccc ccagtcagcc tggccctgct ggtgcctccg
181 gcgctacggg ctgggcaaga tggcggcctt cgggatcttg agctacgaac accggcccct
241 gaagcggccg cggctggggc ctcccgatgt ttaccctcag gaccccaaac agaaggagga
301 tgaactgacg gccttgaatg taaaacaagg tttcaataac cagcctgctg tctctgggga
361 tgagcatggc agtgccaaga acgtcagctt caatcctgcc aagatcagtt ccaacttcag
421 cagcattatt gcagagaaat tacgttgtaa tacccttcct gacactggtc gcaggaagcc
481 ccaagtgaac cagaaggata acttctggct ggtgactgca cgatcccaga gtgccattaa
541 cacttggttc actgacttgg ctggcaccaa gccactcacg caactagcca aaaaggtctc
601 cattttcagt aagaaggaag aggtgtttgg gtacttagcc aaatacacag tgcctgtgat
661 gcgggctgcc tggctcatta agatgacctg tgcctactat gcagcaatct ctgagaccaa
721 ggttaagaag agacatgttg accctttcat ggaatggact cagatcatca ccaagtactt
781 atgggagcag ttacagaaga tggctgaata ctaccggcca gggcctgcag gaagtggggg
841 ctgtggttcc acgatagggc ccttgcccca tgatgtagag gtggcaatcc ggcagtggga
901 ttacaccgag aagctggcca tgttcatgtt tcaggatgga atgctggaca gacatgagtt
961 cctgacctgg gtgcttgagt gttttgagaa gatccgccct ggagaggatg aattgcttaa
1021 actgctgctg cctctgcttc tccgatactc tggggaattt gttcagtctg catacctgtc
1081 ccgccggctt gcctacttct gtacacggag actggccctg cagctggatg gtgtgagcag
1141 tcactcatct catgttatat ctgctcagtc aacaagcacg ctacccacca cccctgctcc
1201 tcagccccca actagcagca caccctcgac tccctttagt gacctgctta tgtgccctca
1261 gcaccggccc ctggtttttg gcctcagctg tatcctacag accatcctcc tgtgctgtcc
1321 tagtgccttg gtttggcact actcactgac tgatagcaga attaagaccg gctcaccact
1381 tgaccacttg cctattgccc cgtccaacct gcccatgcca gagggtaaca gtgccttcac
1441 tcagcaggtc cgtgcaaagt tgcgggagat cgagcagcag atcaaggagc ggggacaggc
1501 agttgaagtt cgctggtctt tcgataaatg ccaggaagct actgcaggct tcaccattgg
1561 acgggtactt catactttgg aagtgctgga cagccatagt tttgaacgct ctgacttcag
1621 caactctctt gactcccttt gtaaccgaat ctttggattg ggacctagca aggatgggca
1681 tgagatctcc tcagatgatg atgctgtggt gtcattgcta tgtgaatggg ctgtcagctg
1741 caagcgttct ggtcggcatc gtgctatggt ggtagccaag ctcctggaga agagacaggc
1801 ggagattgag gctgagcgtt gtggagaatc agaagccgca gatgagaagg gttccatcgc
1861 ctctggctcc ctttctgctc ccagtgctcc cattttccag gatgtcctcc tgcagtttct
1921 ggatacacag gctcccatgc tgacggaccc tcgaagtgag agtgagcggg tggaattctt
1981 taacttagta ctgctgttct gtgaactgat tcgacatgat gttttctccc acaacatgta
2041 tacttgcact ctcatctccc gaggggacct tgcctttgga gcccctggtc cccggcctcc
2101 ctctcccttt gatgatcctg ccgatgaccc agagcacaag gaggctgaag gcagcagcag
2161 cagcaagctg gaagatccag ggctctcaga atctatggac attgacccta gttccagtgt
2221 tctctttgag gacatggaga agcctgattt ctcattgttc tcccctacta tgccctgtga
2281 ggggaagggc agtccatccc ctgagaagcc agatgtcgag aaggaggtga agcccccacc
2341 caaggagaag attgaaggga cccttggggt tctttacgac cagccacgac acgtgcagta
2401 cgccacccat tttcccatcc cccaggagga gtcatgcagc catgagtgca accagcggtt
2461 ggtcgtactg tttggggtgg gaaagcagcg agatgatgcc cgccatgcca tcaagaaaat
2521 caccaaggat atcttgaagg ttctgaaccg caaagggaca gcagaaactg accagcttgc
2581 tcctattgtg cctctgaatc ctggagacct gacattctta ggtggggagg atgggcagaa
2641 gcggcgacgc aaccggcctg aagccttccc cactgctgaa gatatctttg ctaagttcca
2701 gcacctttca cattatgacc aacaccaggt cacggctcag gtctcccgga atgttctgga
2761 gcagatcacg agctttgccc ttggcatgtc ataccacttg cctctggtgc agcatgtgca
2821 gttcatcttc gacctcatgg aatattcact cagcatcagt ggcctcatcg actttgccat
2881 tcagctgctg aatgaactga gtgtagttga ggctgagctg cttctcaaat cctcggatct
2941 ggtgggcagc tacactacta gcctgtgcct gtgcatcgtg gctgtcctgc ggcactatca
3001 tgcctgcctc atcctcaacc aggaccagat ggcacaggtc tttgaggggc tgtgtggcgt
3061 cgtgaagcat gggatgaacc ggtccgatgg ctcctctgca gagcgctgta tccttgctta
3121 tctctatgat ctgtacacct cctgtagcca tttaaagaac aaatttgggg agctcttcag
3181 cgacttttgc tcaaaggtga agaacaccat ctactgcaac gtggagccat cggaatcaaa
3241 tatgcgctgg gcacctgagt tcatgatcga cactctagag aaccctgcag ctcacacctt
3301 cacctacacg gggctaggca agagtcttag tgagaaccct gctaaccgct acagctttgt
3361 ctgcaatgcc cttatgcacg tctgtgtggg gcaccatgat cccgataggg tgaatgacat
3421 cgcaatcctg tgtgcagagc tgaccggcta ttgcaagtca ctgagtgcag aatggctagg
3481 agtgcttaag gccttgtgct gctcctctaa caatggcact tgtggtttca acgatctcct
3541 ctgcaatgtt gatgtcagtg acctatcttt tcatgactcg ctggctactt ttgttgccat
3601 cctcatcgct cggcagtgtt tgctcctgga agatctgatt cgctgtgctg ccatcccttc
3661 actccttaat gctgcttgta gtgaacagga ctctgagcca ggggcccggc ttacctgccg
3721 catcctcctt caccttttca agacaccgca gctcaatcct tgccagtctg atggaaacaa
3781 gcctacagta ggaatccgct cctcctgcga ccgccacctg ctggctgcct cccagaaccg
3841 catcgtggat ggagccgtgt ttgctgttct caaggctgtg tttgtacttg gggatgcgga
3901 actgaaaggt tcaggcttca ctgtgacagg aggaacagaa gaacttccag aggaggaggg
3961 aggaggtggc agtggtggtc ggaggcaggg tggccgcaac atctctgtgg agacagccag
4021 tctggatgtc tatgccaagt acgtgctgcg cagcatctgc caacaggaat gggtaggaga
4081 acgttgcctt aagtctctgt gtgaggacag caatgacctg caagacccag tgttgagtag
4141 tgcccaggcg cagcgcctca tgcagctcat ttgctatcca catcgactgc tggacaatga
4201 ggatggggaa aacccccagc ggcagcgcat aaagcgcatt ctccagaact tggaccagtg
4261 gaccatgcgc cagtcttcct tggagctgca gctcatgatc aagcagaccc ctaacaatga
4321 gatgaactcc ctcttggaga acatcgccaa ggccacaatc gaggttttcc aacagtcagc
4381 agagacaggg tcatcttctg gaagtactgc aagcaacatg cccagcagca gcaagaccaa
4441 gcctgtgctc agctctctag agcgctctgg tgtatggctg gtggcccccc tcattgctaa
4501 actgcccacc tcagtccagg gacatgtgtt aaaggctgct ggggaagaat tggagaaggg
4561 tcagcacctg ggttcctctt cacgcaaaga acgtgatcga caaaagcaga agagcatgtc
4621 cctattgagc cagcagccct tcttatcgct ggtgctaaca tgtctgaaag ggcaggatga
4681 acaacgcgag ggactcctta cctccctcta cagccagatg caccagattg tgaataattg
4741 gcgagatgac cagtacttag atgattgcaa accaaagcag cttatgcatg aggcactcaa
4801 actgcggctc aacctggtgg ggggcatgtt tgacacggtg cagcgcagca cccagcagac
4861 cacggagtgg gccatgctcc tcctggagat catcatcagc ggcactgtcg acatgcagtc
4921 caacaatgag ctcttcacta ctgtgttgga catgctgagc gtgctcatca atgggacatt
4981 ggctgcagac atgtctagca tctcgcaagg tagcatggag gaaaacaagc gtgcatacat
5041 gaacctggcg aagaagttgc agaaggagtt gggggagcgc cagtcagaca gtctggaaaa
5101 ggttcgccag ctgctgccac tgcccaagca gacccgagat gtcatcacgt gtgagccaca
5161 gggctccctt atcgatacca agggcaacaa gattgctggc ttcgattcca tcttcaagaa
5221 ggagggtcta caggtttcca ccaaacagaa gatctcgccc tgggatcttt ttgaggggtt
5281 gaagccgtca gcaccactct cttggggctg gtttggaaca gtccgagtgg accggcgagt
5341 ggctcgagga gaggagcagc agcggttgct gctctaccac acacacctga ggccccggcc
5401 ccgcgcctat tacctggagc cactgccact gcccccagaa gatgaggagc cgcctgctcc
5461 taccctgcta gagcctgaga aaaaggctcc agagcccccc aaaactgaca aaccgggggc
5521 tgctccaccc agtactgagg aacgcaagaa gaagtccacc aagggcaaga aacgcagcca
5581 gccagctacc aagacagagg actatggaat gggcccgggt cggagcggcc cttatggtgt
5641 gacagtgcct ccggacctcc tgcaccaccc aaaccctggt tctataacac accttaacta
5701 caggcaaggc tccataggcc tgtacaccca gaaccagcca ctacctgcag gtggccctcg
5761 tgtggaccca taccgtcctg tgcgcttacc aatgcagaag ctgcccaccc gaccaactta
5821 ccctggagtg ctgcccacaa ccatgactgg cgtcatgggt ttagaaccct cctcttataa
5881 gacctctgtg taccggcagc agcaacctgc ggtgccccaa ggacagcgcc ttcgccaaca
5941 gctccagcag agtcagggca tgttgggaca gtcatctgtc catcagatga ctcccagctc
6001 ttcctacggt ttgcagactt cccagggcta tactccttat gtttctcatg tgggattgca
6061 gcaacacaca ggccctgcag atcctacccg ccacctgcaa cagcggccca gtggctatgt
6121 gcaccagcag gcccccacct atggacatgg actgacctcc actcaaaggt ttcacaccag
6181 acactgcagc agacacccat gataagtacc atgactccaa tgagtgccca gggcgtccag
6241 gcaggcgtcc gttcaacagc catcctacct gagcagcagc agcagcagca acagcagcaa
6301 cagcaacagc agcagcagca gcaacagcaa cagcagcagc agcagcagca gtaccacatc
6361 cggcagcagc agcagcagca gatcctgcgg cagcagcagc aacagcaaca gcagcagcag
6421 cagcagcagc aacagcaaca gcagcagcag caacagcaac aacagcaaca ccagcagcaa
6481 cagcagcaac aggcggctcc tccccaaccc cagccccagt cccagcccca gttccagcgc
6541 caggggcttc agcagaccca gcagcagcaa cagacagcag ctttggtccg gcaacttcaa
6601 caacagctct ctaataccca gccacagccc agtaccaaca tatttggacg ctactgagcc
6661 acctggagga actgcttgtg cactggatgt ggccccaccc tttcctctta attcccaatc
6721 ccattcctgg gctagcacca gtagtggttg gggccctccc ctc
//