LOCUS AK302533 3256 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ56894 complete cds, highly similar to E3
ubiquitin-protein ligase CBL-B (EC 6.3.2.-).
ACCESSION AK302533
VERSION AK302533.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3256)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3256
/clone="TESTI4011522"
/clone_lib="TESTI4"
/db_xref="H-InvDB:HIT000497157"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="testis"
CDS 35..2917
/codon_start=1
/note="highly similar to E3 ubiquitin-protein ligase
CBL-B (EC 6.3.2.-)"
/protein_id="BAG63805.1"
/transl_table=1
/translation="MALAPGSDAHAHLPPLIELKFQMANSMNGRNPGGRGGNPRKGRI
LGIIDAIQDAVGPPKQAAADRRTVEKTWKLMDKVVRLCQNPKLQLKNSPPYILDILPD
TYQHLRLILSKYDDNQKLAQLSENEYFKIYIDSLMKKSKRAIRLFKEGKERMYEEQSQ
DRRNLTKLSLIFSHMLAEIKAIFPNGQFQGDNFRITKADAAEFWRKFFGDKTIVPWKV
FRQCLHEVHQISSGLEAMALKSTIDLTCNDYISVFEFDIFTRLFQPWGSILRNWNFLA
VTHPGYMAFLTYDEVKARLQKYSTKPGSYIFRLSCTRLGQWAIGYVTGDGNILQTIPH
NKPLFQALIDGSREGFYLYPDGRSYNPDLTGLCEPTPHDHIKVTQEQYELYCEMGSTF
QLCKICAENDKDVKIEPCGHLMCTSCLTAWQESDGQGCPFCRCEIKGTEPIIVDPFDP
RDEGSRCCSIIDPFGMPMLDLDDDDDREESLMMNRLANVRKCTDRQNSPVTSPGSSPL
AQRRKPQPDPLQIPHLSLPPVPPRLDLIQKGIVRSPCGSPTGSPKSSPCMVRKQDKPL
PAPPPPLRDPPPPPPERPPPIPPDNRLSRHIHHVESVPSRDPPMPLEAWCPRDVFGTN
QLVGCRLLGEGSPKPGITASSNVNGRHSRVGSDPVLMRKHRRHDLPLEGAKVFSNGHL
GSEEYDVPPRLSPPPPVTTLLPSIKCTGPLANSLSEKTRDPVEEDDDEYKIPSSHPVS
LNSQPSHCHNVKPPVRSCDNGHCMLNGTHGPSSEKKSNIPDLSIYLKGEDAFDALPPS
LPPPPPPARHSLIEHSKPPGSSSRPSSGQDLFLLPSDPFVDLASGQVPLPPARRLPGE
NVKTNRTSQDYDQLPSCSDGSQAPARPPKPRPRRTAPEIHHRKPHGPEAALENVDAKI
AKLMGEGYAFEEVKRALEIAQNNVEVARSILREFAFPPPVSPRLNL"
BASE COUNT 933 a 785 c 706 g 832 t
ORIGIN
1 aagcgaagca ggaagtcccg cctttcagcc ccagatggct ttggctcccg gttccgacgc
61 tcacgcccac ctgccacccc ttattgaact aaaattccag atggcaaact caatgaatgg
121 cagaaaccct ggtggtcgag gaggaaatcc ccgaaaaggt cgaattttgg gtattattga
181 tgctattcag gatgcagttg gaccccctaa gcaagctgcc gcagatcgca ggaccgtgga
241 gaagacttgg aagctcatgg acaaagtggt aagactgtgc caaaatccca aacttcagtt
301 gaaaaatagc ccaccatata tacttgatat tttgcctgat acatatcagc atttacgact
361 tatattgagt aaatatgatg acaaccagaa acttgcccaa ctcagtgaga atgagtactt
421 taaaatctac attgatagcc ttatgaaaaa gtcaaaacgg gcaataagac tctttaaaga
481 aggcaaggag agaatgtatg aagaacagtc acaggacaga cgaaatctca caaaactgtc
541 ccttatcttc agtcacatgc tggcagaaat caaagcaatc tttcccaatg gtcaattcca
601 gggagataac tttcgtatca caaaagcaga tgctgctgaa ttctggagaa agttttttgg
661 agacaaaact atcgtaccat ggaaagtatt cagacagtgc cttcatgagg tccaccagat
721 tagctctggc ctggaagcaa tggctctaaa atcaacaatt gatttaactt gcaatgatta
781 catttcagtt tttgaatttg atatttttac caggctgttt cagccttggg gctctatttt
841 gcggaattgg aatttcttag ctgtgacaca tccaggttac atggcatttc tcacatatga
901 tgaagttaaa gcacgactac agaaatatag caccaaaccc ggaagctata ttttccggtt
961 aagttgcact cgattgggac agtgggccat tggctatgtg actggggatg ggaatatctt
1021 acagaccata cctcataaca agcccttatt tcaagccctg attgatggca gcagggaagg
1081 attttatctt tatcctgatg ggaggagtta taatcctgat ttaactggat tatgtgaacc
1141 tacacctcat gaccatataa aagttacaca ggaacaatat gaattatatt gtgaaatggg
1201 ctccactttt cagctctgta agatttgtgc agagaatgac aaagatgtca agattgagcc
1261 ttgtgggcat ttgatgtgca cctcttgcct tacggcatgg caggagtcgg atggtcaggg
1321 ctgccctttc tgtcgttgtg aaataaaagg aactgagccc ataatcgtgg acccctttga
1381 tccaagagat gaaggctcca ggtgttgcag catcattgac ccctttggca tgccgatgct
1441 agacttggac gacgatgatg atcgtgagga gtccttgatg atgaatcggt tggcaaacgt
1501 ccgaaagtgc actgacaggc agaactcacc agtcacatca ccaggatcct ctccccttgc
1561 ccagagaaga aagccacagc ctgacccact ccagatccca catctaagcc tgccacccgt
1621 gcctcctcgc ctggatctaa ttcagaaagg catagttaga tctccctgtg gcagcccaac
1681 aggttcacca aagtcttctc cttgcatggt gagaaaacaa gataaaccac tcccagcacc
1741 acctcctccc ttaagagatc ctcctccacc gccacctgaa agacctccac caatcccacc
1801 agacaataga ctgagtagac acatccatca tgtggaaagc gtgccttcca gagacccgcc
1861 aatgcctctt gaagcatggt gccctcggga tgtgtttggg actaatcagc ttgtgggatg
1921 tcgactccta ggggagggct ctccaaaacc tggaatcaca gcgagttcaa atgtcaatgg
1981 aaggcacagt agagtgggct ctgacccagt gcttatgcgg aaacacagac gccatgattt
2041 gcctttagaa ggagctaagg tcttttccaa tggtcacctt ggaagtgaag aatatgatgt
2101 tcctccccgg ctttctcctc ctcctccagt taccaccctc ctccctagca taaagtgtac
2161 tggtccgtta gcaaattctc tttcagagaa aacaagagac ccagtagagg aagatgatga
2221 tgaatacaag attccttcat cccaccctgt ttccctgaat tcacaaccat ctcattgtca
2281 taatgtaaaa cctcctgttc ggtcttgtga taatggtcac tgtatgctga atggaacaca
2341 tggtccatct tcagagaaga aatcaaacat ccctgactta agcatatatt taaagggtga
2401 agatgctttt gatgccctcc ctccatctct cccacctccc ccacctcctg caaggcatag
2461 tctcattgaa cattcaaaac ctcctggctc cagtagccgg ccatcctcag gacaggatct
2521 ttttcttctt ccttcagatc cctttgttga tctagcaagt ggccaagttc ctttgcctcc
2581 tgctagaagg ttaccaggtg aaaatgtcaa aactaacaga acatcacagg actatgatca
2641 gcttccttca tgttcagatg gttcacaggc accagccaga ccccctaaac cacgaccgcg
2701 caggactgca ccagaaattc accacagaaa accccatggg cctgaggcgg cattggaaaa
2761 tgtcgatgca aaaattgcaa aactcatggg agagggttat gcctttgaag aggtgaagag
2821 agccttagag atagcccaga ataatgtcga agttgcccgg agcatcctcc gagaatttgc
2881 cttccctcct ccagtatccc cacgtctaaa tctatagcag ccagaactgt agacaccaaa
2941 atggaaagca atcgatgtat tccaagagtg tggaaataaa gagaactgag atggaattca
3001 agagagaagt gtctcctcct cgtgtagcag cttgagaaga ggcttgggag tgcagcttct
3061 caaaggagac cgatgcttgc tcaggatgtc gacagctgtg gcttccttgt ttttgctagc
3121 catattttta aatcagggtt gaactgacaa aaataattta aagacgttta cttcccttga
3181 actttgaacc tgtgaaatgc tttaccttgt ttacagtttg gcaaagttgc agtttgttct
3241 tgtttttagt ttagtt
//