LOCUS AK302519 2067 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ52365 complete cds, highly similar to
Galactocerebrosidase precursor (EC 3.2.1.46).
ACCESSION AK302519
VERSION AK302519.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2067)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2067
/clone="TESTI4010488"
/clone_lib="TESTI4"
/db_xref="H-InvDB:HIT000497143"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="testis"
CDS 157..1461
/codon_start=1
/note="highly similar to Galactocerebrosidase precursor
(EC 3.2.1.46)"
/protein_id="BAG63793.1"
/transl_table=1
/translation="MLNYQGLQRVKIIASDNLWESISASMLLDAELFKVVDVIGAHYP
GTHSAKDAKLTGKKLWSSEDFSTLNSDMGAGCWGRILNQNYINGYMTSTIAWNLVASY
YEQLPYGRCGLMTAQEPWSGHYVVESPVWVSAHTTQFTQPGWYYLKTVGHLEKGGSYV
ALTDGLGNLTIIIETMSHKHSKCIRPFLPYFNVSQQFATFVLKGSFSEIPELQVWYTK
LGKTSERFLFKQLDSLWLLNSDGSFTLSLHEDELFTLTTLTTGRKGSYPLPPKSQPFP
STYKDDFNVDYPFFSEAPNFADQTGVFEYFTNIEDPGEHHFTLRQVLNQRPITWAADA
SNTISIIGDYNWTNLTIKCDVYIETPDTGGVFIAGRVNKGGILIRSARGIFFWIFANG
SYRVTGDLAGWIIYALGRVEVTAKKWYTLTLTIKVAGRRKKT"
BASE COUNT 577 a 444 c 457 g 589 t
ORIGIN
1 gagatgacac ctctccctgg gttgattagg gaagtctttt agaggaggtg atgtgtaatt
61 tgaatcttca agaacggatg gataggaact ctccactttg ctaaggcagg ggcatagcat
121 gtgcaaagaa ctgaagattg agagatatta agaaaaatgc tgaattatca aggtctccag
181 cgagtgaaaa tcatagcaag tgataatctc tgggagtcca tctctgcatc catgctcctt
241 gatgccgaac tcttcaaggt ggttgatgtt ataggggctc attatcctgg aacccattca
301 gcaaaagatg caaagttgac tgggaagaag ctttggtctt ctgaagactt tagcacttta
361 aatagtgaca tgggtgcagg ctgctggggt cgcattttaa atcagaatta tatcaatggc
421 tatatgactt ccacaatcgc atggaattta gtggctagtt actatgaaca gttgccttat
481 gggagatgcg ggttgatgac ggcccaagag ccatggagtg ggcactacgt ggtagaatct
541 cctgtctggg tatcagctca taccactcag tttactcaac ctggctggta ttacctgaag
601 acagttggcc atttagagaa aggaggaagc tacgtagctc tgactgatgg cttagggaac
661 ctcaccatca tcattgaaac catgagtcat aaacattcta agtgcatacg gccatttctt
721 ccttatttca atgtgtcaca acaatttgcc acctttgttc ttaagggatc ttttagtgaa
781 ataccagagc tacaggtatg gtataccaaa cttggaaaaa catccgaaag atttcttttt
841 aagcagctgg attctctatg gctccttaac agcgatggca gtttcacact gagcctgcat
901 gaagatgagc tgttcacact caccactctc accactggtc gcaaaggcag ctacccgctt
961 cctccaaaat cccagccctt cccaagtacc tataaggatg atttcaatgt tgattaccca
1021 ttttttagtg aagctccaaa ctttgctgat caaactggtg tatttgaata ttttacaaat
1081 attgaagacc ctggcgagca tcacttcacg ctacgccaag ttctcaacca gagacccatt
1141 acgtgggctg ccgatgcatc caacacaatc agtattatag gagactacaa ctggaccaat
1201 ctgactataa agtgtgatgt ttacatagag acccctgaca caggaggtgt gttcattgca
1261 ggaagagtaa ataaaggtgg tattttgatt agaagtgcca gaggaatttt cttctggatt
1321 tttgcaaatg gatcttacag ggttacaggt gatttagctg gatggattat atatgcttta
1381 ggacgtgttg aagttacagc aaaaaaatgg tatacactca cgttaactat taaggtggct
1441 ggaagaagga agaaaacctg atatagccaa tgctgttgta gtcgtttcct cagcctcatc
1501 tcactgtgct gtggtctgtc ctcacatgtg cactgagtct cgctctgtca cccaggctgg
1561 aatgcattgg ctccatctca gctcactgca acctccgctt cccgggttca agtgattctc
1621 ctgcctcagc ctcctgagta gctgggatta cagttctggc ggctagaagt tcaagatcaa
1681 gatgttggca ggactggttc ttccaggctt ctctcctttg cttatagatg accactgtct
1741 tcttgcatcc ccacatcatc ttcaattctg tatatgtcta tgccctaatt ttctcttctt
1801 ataaggatac cagtcacact ggattagggc ccaccccagt gacctatttt aacttaatta
1861 tacctcttta aagaccctag ctccacatat agtcacattc tgaggtactg agggttgact
1921 tcaacatagg aatttaaagg taggggtagg gacacaattc agctcgtagc taagacactt
1981 tgcatggagt ccggagtctt tcaaaatcag gccttgatag ttcaaaagta aatgctggaa
2041 atgacctaca taggaaaaaa aaaagat
//