LOCUS AK302443 1993 bp mRNA linear HUM 31-JUL-2008
DEFINITION Homo sapiens cDNA FLJ54262 complete cds, highly similar to
N-acetylglucosamine-6-sulfatase precursor (EC 3.1.6.14).
ACCESSION AK302443
VERSION AK302443.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1993)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1993
/clone="TESTI4004642"
/clone_lib="TESTI4"
/db_xref="H-InvDB:HIT000497067"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="testis"
CDS 129..1727
/codon_start=1
/note="highly similar to N-acetylglucosamine-6-sulfatase
precursor (EC 3.1.6.14)"
/protein_id="BAG63740.1"
/transl_table=1
/translation="MRLLPLAPGRLRRGSPRHLPSCSPALLLLVLGGCLGVFGVAAGT
RRPNVVLLLTDDQDEVLGGMYVPSALCCPSRASILTGKYPHNHHVVNNTLEGNCSSKS
WQKIQEPNTFPAILRSMCGYQTFFAGKYLNEYGAPDAGGLEHVPLGWSYWYALEKNSK
YYNYTLSINGKARKHGENYSVDYLTDVLANVSLDFLDYKSNFEPFFMMIATPAPHSPW
TAAPQYQKAFQNVFAPRNKNFNIHGTNKHWLIRQAKTPMTNSSIQFLDNAFRKRWQTL
LSVDDLVEKLVKRLEFTGELNNTYIFYTSDNGYHTGQFSLPIDKRQLYEFDIKVPLLV
RGPGIKPNQTSKMLVANIDLGPTILDIAGYDLNKTQMDGMSLLPILRGASNLTWRSDV
LVEYQGEGRNVTDPTCPSLSPGVSQCFPDCVCEDAYNNTYACVRTMSALWNLQYCEFD
DQEVFVEVYNLTADPDQITNIAKTIDPELLGKMNYRLMMLQSCSGPTCRTPGVFDPGY
RFDPRLMFSNRGSVRTRRFSKHLL"
BASE COUNT 482 a 516 c 484 g 511 t
ORIGIN
1 ctgactccag ccaccggcct tcaaggcacg gctttttatt ccttcggctg gtcggcctct
61 cgcccttcag ctacctgtgc gtccctccgt cccgtcccgt cccggggtca ccccggagcc
121 tgtccgctat gcggctcctg cctctagccc caggtcggct ccggcggggc agcccccgcc
181 acctgccctc ctgcagccca gcgctgctac tgctggtgct gggcggctgc ctgggggtct
241 tcggggtggc tgcgggaacc cggaggccca acgtggtgct gctcctcacg gacgaccagg
301 acgaagtgct cggcggcatg tatgtgccaa gtgctctctg ctgccccagc agagccagta
361 tcctgacagg aaagtaccca cataatcatc acgttgtgaa caacactctg gaggggaact
421 gcagtagtaa gtcctggcag aagatccaag aaccaaatac tttcccagca attctcagat
481 caatgtgtgg ttatcagacc ttttttgcag ggaaatattt aaatgagtac ggagccccag
541 atgcaggtgg actagaacac gttcctctgg gttggagtta ctggtatgcc ttggaaaaga
601 attctaagta ttataattac accctgtcta tcaatgggaa ggcacggaag catggtgaaa
661 actatagtgt ggactacctg acagatgttt tggctaatgt ctccttggac tttctggact
721 acaagtccaa ctttgagccc ttcttcatga tgatcgccac tccagcgcct cattcgcctt
781 ggacagctgc acctcagtac cagaaggctt tccagaatgt ctttgcacca agaaacaaga
841 acttcaacat ccatggaacg aacaagcact ggttaattag gcaagccaag actccaatga
901 ctaattcttc aatacagttt ttagataatg catttaggaa aaggtggcaa actctcctct
961 cagttgatga ccttgtggag aaactggtca agaggctgga gttcactggg gagctcaaca
1021 acacttacat cttctatacc tcagacaatg gctatcacac aggacagttt tccttgccaa
1081 tagacaagag acagctgtat gagtttgata tcaaagttcc actgttggtt cgaggacctg
1141 ggatcaaacc aaatcagaca agcaagatgc tggttgccaa cattgacttg ggtcctacta
1201 ttttggacat tgctggctac gacctaaata agacacagat ggatgggatg tccttattgc
1261 ccattttgag aggtgccagt aacttgacct ggcgatcaga tgtcctggtg gaataccaag
1321 gagaaggccg taacgtcact gacccaacat gcccttccct gagtcctggc gtatctcaat
1381 gcttcccaga ctgtgtatgt gaagatgctt ataacaatac ctatgcctgt gtgaggacaa
1441 tgtcagcatt gtggaatttg cagtattgcg agtttgatga ccaggaggtg tttgtagaag
1501 tctataatct gactgcagac ccagaccaga tcactaacat tgctaaaacc atagacccag
1561 agcttttagg aaagatgaac tatcggttaa tgatgttaca gtcctgttct gggccaacct
1621 gtcgcactcc aggggttttt gaccccggat acaggtttga cccccgtctc atgttcagca
1681 atcgcggcag tgtcaggact cgaagatttt ccaaacatct tctgtagcga cctcacacag
1741 cctctgcaga tggatccctg cacgcctctt tctgatgaag tgattgtagt aggtgtctgt
1801 agctagtctt caagaccaca cctggaagag tttctgggct ggctttaagt cctgtttgaa
1861 aaagcaaccc agtcagctga cttcctcgtg caatgtgtta aactgtgaac tctgcccatg
1921 tgtcaggagt ggctgtctct ggtctcttcc tttagctgac aaggacactc ctgaggtctt
1981 tgttctcact gta
//