LOCUS AK302321 1749 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ51565 complete cds, highly similar to
Pyruvate dehydrogenase (lipoamide) kinase isozyme 1 (EC 2.7.11.2).
ACCESSION AK302321
VERSION AK302321.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1749)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1749
/clone="TESTI2048328"
/clone_lib="TESTI2"
/db_xref="H-InvDB:HIT000496945"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="testis"
CDS 381..1463
/codon_start=1
/note="highly similar to Pyruvate dehydrogenase
(lipoamide) kinase isozyme 1 (EC 2.7.11.2)"
/protein_id="BAH13672.1"
/transl_table=1
/translation="MFLRQELPVRLANIMKEISLLPDNLLRTPSVQLVQSWYIQSLQE
LLDFKDKSAEDAKAIYDFTDTVIRIRNRHNDVIPTMAQGVIEYKESFGVDPVTSQNVQ
YFLDRFYMSRISIRMLLNQHSLLFGGKGKGSPSHRKHIGSINPNCNVLEVIKDGYENA
RRLCDLYYINSPELELEELNAKSPGQPIQVVYVPSHLYHMVFELFKNAMRATMEHHAN
RGVYPPIQVHVTLGNEDLTVKMSDRGGGVPLRKIDRLFNYMYSTAPRPRVETSRAVPL
AGFGYGLPISRLYAQYFQGDLKLYSLEGYGTDAVIYIKALSTDSIERLPVYNKAAWKH
YNTNHEADDWCVPSREPKDMTTFRSA"
BASE COUNT 475 a 397 c 419 g 458 t
ORIGIN
1 tataatatgc tcctttttca ggtaaagaga cggaggctca gagaggttaa acagctcact
61 caaaggtaca gagccagtac atggcagtac atctcttctc ccggggccgc cgtcgcggtg
121 atactattta gggccacacg tcccagtgtt cagctttcgt atttacgtac tactgtttgg
181 caagcgggga ctcctgaagc tctctgaccc tggaaaatgc gtggcttgcg tcacagagga
241 gcgtttctgg aacgcgctcg cagcacgaca ccgcggtgtg tgcaagcgga acgccgccct
301 gtccttgagc cttttggctg agattgggag ggcagaggaa gcaggaagga tcagtgaatg
361 cttgtgaaaa gacctcattt atgtttctgc ggcaagagtt gcctgtcaga ctggcaaata
421 taatgaaaga aataagtctc cttccagata atcttctcag gacaccatcc gttcaattgg
481 tacaaagctg gtatatccag agtcttcagg agcttcttga ttttaaggac aaaagtgctg
541 aggatgctaa agctatttat gactttacag atactgtgat acggatcaga aaccgacaca
601 atgatgtcat tcccacaatg gcccagggtg tgattgaata caaggagagc tttggggtgg
661 atcctgtcac cagccagaat gttcagtact ttttggatcg attctacatg agtcgcattt
721 caattagaat gttactcaat cagcactctt tattgtttgg tggaaaaggc aaaggaagtc
781 catctcatcg aaaacacatt ggaagcataa atccaaactg caatgtactt gaagttatta
841 aagatggcta tgaaaatgct aggcgtctgt gtgatttgta ttatattaac tctcccgaac
901 tagaacttga agaactaaat gcaaaatcac caggacagcc aatacaagtg gtttatgtac
961 catcccatct ctatcacatg gtgtttgaac ttttcaagaa tgcaatgaga gccactatgg
1021 aacaccatgc caacagaggt gtttaccccc ctattcaagt tcatgtcacg ctgggtaatg
1081 aggatttgac tgtgaagatg agtgaccgag gaggtggcgt tcctttgagg aaaattgaca
1141 gacttttcaa ctacatgtat tcaactgcac caagacctcg tgttgagacc tcccgcgcag
1201 tgcctctggc tggttttggt tatggattgc ccatatcacg tctttacgca caatacttcc
1261 aaggagacct gaagctgtat tccctagagg gttacgggac agatgcagtt atctacatta
1321 aggctctgtc aacagactca atagaaagac tcccagtgta taacaaagct gcctggaagc
1381 attacaacac caaccacgag gctgatgact ggtgcgtccc cagcagagaa cccaaagaca
1441 tgacgacgtt ccgcagtgcc tagacacact tgggacatcg gaaaatccaa atgtggcttt
1501 tgtattaaat ttggaagatt cttcagtttt gggacttgga ctggctttcc ttgcccctca
1561 gcttgcagat ggcctgttgt gggaccttgt gatcatgaac tcctcctccc acatctgatg
1621 gagctaccaa tcatcagatc tcctctgcct ctggggtgcg tctcaggctg gccaatcacg
1681 ttcttcatcc acgtggacta tgactggccc tagggagaaa acatggagat atgaatgact
1741 gtgtggagc
//