LOCUS AK302242 1638 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ51387 complete cds, highly similar to
Asparagine synthetase (glutamine-hydrolyzing) (EC 6.3.5.4).
ACCESSION AK302242
VERSION AK302242.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1638)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1638
/clone="TESTI2041599"
/clone_lib="TESTI2"
/db_xref="H-InvDB:HIT000496866"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="testis"
CDS 74..1510
/codon_start=1
/note="highly similar to Asparagine synthetase
(glutamine-hydrolyzing) (EC 6.3.5.4)"
/protein_id="BAG63594.1"
/transl_table=1
/translation="MQQHFEFEYQTKVDGEIILHLYDKGGIEQTICMLDGVFAFVLLD
TANKKVFLGRDTYGVRPLFKAMTEDGFLAVCSEAKGLVTLKHSATPFLKVEPFLPGHY
EVLDLKPNGKVASVEMVKYHHCRDEPLHALYDNVEKLFPGFEIETVKNNLRILFNNAV
KKRLMTDRRIGCLLSGGLDSSLVAATLLKQLKEAQVQYPLQTFAIGMEDSPDLLAARK
VADHIGSEHYEVLFNSEEGIQALDEVIFSLETYDITTVRASVGMYLISKYIRKNTDSV
VIFSGEGSDELTQGYIYFHKAPSPEKAEEESERLLRELYLFDVLRADRTTAAHGLELR
VPFLDHRFSSYYLSLPPEMRIPKNGIEKHLLRETFEDSNLIPKEILWRPKEAFSDGIT
SVKNSWFKILQEYVEHQVDDAMMANAAQKFPFNTPKTKEGYYYRQIFERHYPGRADWL
SHYWMPKWINATDPSARTLTHYKSAVKA"
BASE COUNT 474 a 325 c 391 g 448 t
ORIGIN
1 gaagcatcga gagggaaccg cggccggcag gccggaccgc tccggagagg aggcagcggc
61 agtttgagcc cctatgcaac agcattttga atttgaatac cagaccaaag tggatggtga
121 gataatcctt catctttatg acaaaggagg aattgagcaa acaatttgta tgttggatgg
181 tgtgtttgca tttgttttac tggatactgc caataagaaa gtgttcctgg gtagagatac
241 atatggagtc agacctttgt ttaaagcaat gacagaagat ggatttttgg ctgtatgttc
301 agaagctaaa ggtcttgtta cattgaagca ctccgcgact ccctttttaa aagtggagcc
361 ttttcttcct ggacactatg aagttttgga tttaaagcca aatggcaaag ttgcatccgt
421 ggaaatggtt aaatatcatc actgtcggga tgaacccctg cacgccctct atgacaatgt
481 ggagaaactc tttccaggtt ttgagataga aactgtgaag aacaacctca ggatcctttt
541 taataatgct gtaaagaaac gtttgatgac agacagaagg attggctgcc ttttatcagg
601 gggcttggac tccagcttgg ttgctgccac tctgttgaag cagctgaaag aagcccaagt
661 acagtatcct ctccagacat ttgcaattgg catggaagac agccccgatt tactggctgc
721 tagaaaggtg gcagatcata ttggaagtga acattatgaa gtccttttta actctgagga
781 aggcattcag gctctggatg aagtcatatt ttccttggaa acttatgaca ttacaacagt
841 tcgtgcttca gtaggtatgt atttaatttc caagtatatt cggaagaaca cagatagcgt
901 ggtgatcttc tctggagaag gatcagatga acttacgcag ggttacatat attttcacaa
961 ggctccttct cctgaaaaag ccgaggagga gagtgagagg cttctgaggg aactctattt
1021 gtttgatgtt ctccgcgcag atcgaactac tgctgcccat ggtcttgaac tgagagtccc
1081 atttctagat catcgatttt cttcctatta cttgtctctg ccaccagaaa tgagaattcc
1141 aaagaatggg atagaaaaac atctcctgag agagacgttt gaggattcca atctgatacc
1201 caaagagatt ctctggcgac caaaagaagc cttcagtgat ggaataactt cagttaagaa
1261 ttcctggttt aagattttac aggaatacgt tgaacatcag gttgatgatg caatgatggc
1321 aaatgcagcc cagaaatttc ccttcaatac tcctaaaacc aaagaaggat attactaccg
1381 tcaaatcttt gaacgccatt acccaggccg ggctgactgg ctgagccatt actggatgcc
1441 caagtggatc aatgccactg acccttctgc ccgcacgctg acccactaca agtcagctgt
1501 caaagcttag gtggtcttta tgctgtaatg tgaaagcaaa tatttcttcg tgttggatgg
1561 ggactgtggg tagatagggg aacaatgaga gtcaactcag gctaacttgg gtgtgaaaaa
1621 aataaaagtc ctaaatct
//