LOCUS AK302144 1427 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ50035 complete cds.
ACCESSION AK302144
VERSION AK302144.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1427)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1427
/clone="TESTI2033964"
/clone_lib="TESTI2"
/db_xref="H-InvDB:HIT000496768"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="testis"
CDS 86..1186
/codon_start=1
/protein_id="BAG63516.1"
/transl_table=1
/translation="MSWNISHPQLSDKLFGKNGVLEEQKSPGFKKRETEVYVGNLPLD
ISKEEILYLLKDFNPLDVHKIQNGCKCFAFVDLGSMQKVTLAIQELNGKLFHKRKLFV
NTSKRPPKRTPDMIQQPRAPLVLEKASGEGFGKTAAIIQLAPKAPVDLCETEKLRAAF
FAVPLEMRGSFLVLLLRECFRDLSWLALIHSVRGEAGLLVTSIVPKTPFFWAMHITEA
LHQNMQALFSTLAQAEEQQPYLEGSTVMRGTRCLAEYHLGDYGHAWNRCWVLDRVDTW
AVVMFIDFGQLATIPVQSLRSLDSDDFWTIPPLTQPFMLEKDILSSYEVVHRILKGKI
TGALNSAVTAPASNLAVVPPLLPLGCLQQAAA"
BASE COUNT 313 a 377 c 391 g 346 t
ORIGIN
1 aaggcgcgaa ggctgggcgt cgcgatcgca ggtgcacgcg cgtggcccgc gagacgagga
61 gatcctgttg gaaagcaact gcagcatgtc ctggaacatt agtcaccccc aactctctga
121 taaactgttt gggaagaatg gagtgttgga ggagcagaaa tctccaggat tcaagaaaag
181 agagacagag gtgtatgttg gcaatcttcc actggatatt tctaaggagg aaattctgta
241 ccttctaaag gacttcaacc ctcttgatgt ccacaaaatc cagaatggct gcaaatgctt
301 tgcatttgta gatctgggct ccatgcagaa agtgacactt gcaatccagg agctgaatgg
361 taaactcttc cacaagcgaa aactgttcgt gaatacaagc aaaaggcccc ccaagaggac
421 ccctgatatg atccagcagc ctcgggcccc gctggtgttg gagaaggctt ctggtgaagg
481 atttggcaaa accgccgcta ttatacagct cgctcctaaa gctcctgttg acctgtgtga
541 gacagagaaa ctgagggcag ccttctttgc agtcccgttg gaaatgagag ggtccttcct
601 ggtgctgctc ctgagggaat gcttccgaga cctgagctgg ctggcactca tccatagcgt
661 ccgtggggag gcggggctgc tggtgacgag tatcgtcccg aagaccccgt ttttctgggc
721 catgcacatc actgaggctc tgcaccagaa catgcaggct ctgtttagca ccctggctca
781 ggcggaggag cagcagccct acctggaggg ctccaccgtt atgcgcggga ctcgctgtct
841 ggcagagtac cacctggggg attatggaca cgcctggaac aggtgttggg tgctggacag
901 ggtggacacc tgggctgtgg tcatgttcat tgattttgga cagttggcca ccatccctgt
961 gcagtctctg cgcagcctag acagcgacga cttctggacc atcccacccc tgactcagcc
1021 attcatgctg gagaaagaca ttttgagttc gtatgaggtt gtccatcgaa tcctcaaagg
1081 gaaaatcact ggtgctttga actcggcggt aactgctcct gcatctaact tggctgttgt
1141 ccctccactc ctgcccttgg ggtgtctgca gcaggctgct gcctaggcct ggacacagta
1201 tttacctggc attcttttag atcaaacgaa ctggttatct gaggggttgg gtgaactggc
1261 tcgggtgggc cttccttccc accaaggctt tgtctttctc ttccagttgc acatcctaaa
1321 gtttgaagag tctaaataac ggggcttccc tcagcatgtt ccctctcctg tttgccacgg
1381 atccagaggc cacctgccct gtcttctcgt acccctttca ctcttga
//