LOCUS AK302142 2244 bp mRNA linear HUM 31-JUL-2008
DEFINITION Homo sapiens cDNA FLJ61690 complete cds, highly similar to HECT
domain and RCC1-like domain-containing protein 5.
ACCESSION AK302142
VERSION AK302142.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2244)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2244
/clone="TESTI2033590"
/clone_lib="TESTI2"
/db_xref="H-InvDB:HIT000496766"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="testis"
CDS 112..1848
/codon_start=1
/note="highly similar to HECT domain and RCC1-like
domain-containing protein 5"
/protein_id="BAG63515.1"
/transl_table=1
/translation="MIAGGNQSILLWIKKENSYVNLKRTIPTLNEGTVKRWIADVETK
RWQSTKREIQEIFSSPACLTGSFLRKRRTTEMMPVYLDLNKARNIFKELTQKDWITNM
ITTCLKDNLLKRLPFHSPPQEALEIFFLLPECPMMHISNNWESLVVPFAKVVCKMSDQ
SSLVLEEYWATLQESTFSKLVQMFKTAVICQLDYWDESAEENGNVQALLEMLKKLHRV
NQVKCQLPESIFQVDELLHRLNFFVEVCRRYLWKMTVDASENVQCCVIFSHFPFIFNN
LSKIKLLHTDTLLKIESKKHKAYLRSAAIEEERESEFALRPTFDLTVRRNHLIEDVLN
QLSQFENEDLRKELWVSFSGEIGYDLGGVKKEFFYCLFAEMIQPEYGMFMYPEGASCM
WFPVKPKFEKKRYFFFGVLCGLSLFNCNVANLPFPLALFKKLLDQMPSLEDLKELSPD
LGKNLQTLLDDEGDNFEEVFYIHFNVHWDRNDTNLIPNGSSITVNQTNKRDYVSKYIN
YIFNDSVKAVYEEFRRGFYKMCDGDIIKLFHPEELKDVIVGNTDYDWKTFEKNARYEP
GYNSSHPTIVMF"
BASE COUNT 724 a 391 c 470 g 659 t
ORIGIN
1 aaaagcttag tggtgacctc aagtaacaaa atctactcat ttcttgtagg aaaacgaaca
61 gagttatttc cctaagcagc ataaagccat acctcagaaa aggagttaat aatgattgct
121 ggagggaatc aaagcatttt gctctggata aagaaagaga attcatatgt taatctgaag
181 aggacaattc ctactctgaa tgaagggact gtaaagagat ggattgctga tgtggagact
241 aaacggtggc agagcacaaa aagggaaatc caagagatat tttcatctcc tgcttgtcta
301 actggaagtt ttttaaggaa aagaagaact acagaaatga tgcctgttta tttggactta
361 aataaagcaa gaaacatctt caaggagtta acccaaaagg actggattac taacatgata
421 accacctgcc tcaaagataa tctgctcaaa agacttccat ttcattctcc accccaagaa
481 gctttagaaa ttttcttcct tctcccagaa tgtcctatga tgcatatttc caacaactgg
541 gagagccttg tggttccatt tgcaaaggtt gtttgtaaaa tgagtgacca gtcttcactg
601 gttctggaag agtattgggc aactctgcaa gaatccactt tcagcaaact ggtccagatg
661 tttaaaacag ccgtcatatg ccagttggat tactgggatg aaagtgctga ggagaatggt
721 aatgttcaag ctctcctaga aatgttgaag aagctgcaca gggtaaacca ggtgaaatgt
781 caactacctg aaagtatttt ccaagtagac gaactcttgc accgtctcaa tttttttgta
841 gaagtatgca gaaggtactt gtggaaaatg actgtggacg cttcagaaaa tgtacaatgc
901 tgcgtcatat tcagtcactt tccatttatc tttaataatc tgtcgaaaat taaactacta
961 catacagaca cacttttaaa aatagagagt aaaaaacata aagcttatct taggtcggca
1021 gcaattgagg aagaaagaga gtctgaattc gctttgaggc ccacgtttga tctaacagtc
1081 agaaggaatc acttgattga ggatgttttg aatcagctaa gtcaatttga gaatgaagac
1141 ctgaggaaag agttatgggt ttcatttagt ggagaaattg ggtatgacct cggaggagtc
1201 aagaaagagt tcttctactg tctgtttgca gagatgatcc agccggaata tgggatgttc
1261 atgtatcctg aaggggcttc ctgcatgtgg tttcctgtca agcctaaatt tgagaagaaa
1321 agatacttct tttttggggt tctatgtgga ctttccctgt tcaattgcaa tgttgccaac
1381 ctccctttcc cactggcact gtttaagaaa cttttggacc aaatgccatc attggaagac
1441 ttgaaagaac tcagtcctga tttgggaaag aatttgcaaa cacttctgga tgatgaaggt
1501 gataactttg aggaagtatt ttacatccat tttaatgtgc actgggacag aaacgacaca
1561 aacttaattc ctaatggaag tagcataact gtcaaccaga ctaacaagag agactatgtt
1621 tctaagtata tcaattacat tttcaacgac tctgtaaagg cggtttatga agaatttcgg
1681 agaggatttt ataaaatgtg cgacggagac attatcaaat tattccaccc cgaagaactg
1741 aaggatgtga ttgttggaaa tacagattat gattggaaaa catttgaaaa gaatgcacgt
1801 tatgaaccag gatataacag ttcacatccc accatagtga tgttttagaa ggctttccac
1861 aaattgactc tggaagaaaa gaaaaaattc cttgtatttc ttacaggaac tgacagacta
1921 caaatgaaag atttaaataa tatgaaaata acattttgct gtcctgaaag ttggaatgaa
1981 agagacccta taagagcact gacatgtttc agtgtcctct tcctccctaa atattctaca
2041 atggaaacag ttgaagaagc gcttcaagaa gccatcaaca acaacagagg atttggctga
2101 ccagcttgct tgtccaacag ccttattttg ttgttgttat cgttgttgtt gttgttgttg
2161 ttgttgtttc tctactttgt tttgttttag gcttttagca gcctgaagcc atggtttttc
2221 atttctgtct ctagtgataa gcag
//