LOCUS AK301841 1598 bp mRNA linear HUM 31-JUL-2008
DEFINITION Homo sapiens cDNA FLJ61445 complete cds, highly similar to Protein
transport protein Sec61 subunit alpha isoform 2.
ACCESSION AK301841
VERSION AK301841.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1598)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1598
/clone="TESTI2010001"
/clone_lib="TESTI2"
/db_xref="H-InvDB:HIT000496465"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="testis"
CDS 101..1465
/codon_start=1
/note="highly similar to Protein transport protein Sec61
subunit alpha isoform 2"
/protein_id="BAG63284.1"
/transl_table=1
/translation="MGIKFLEVIKPFCAVLPEIQKPERKIPLFGIMSSDSADPFYWMR
VILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEVGDTPKDRALFNGAQKLFGMIIT
IGQAIVYVMTGMYGDPAEMGAGICLLIIIQLFVAGLIVLLLDELLQKGYGLGSGISLF
IATNICETIVWKAFSPTTINTGRGTEFEGAVIALFHLLATRTDKVRALREAFYRQNLP
NLMNLIATVFVFAVVIYFQGFRVDLPIKSARYRGQYSSYPIKLFYTSNIPIILQSALV
SNLYVISQMLSVRFSGNFLVNLLGQWADVSGGGPARSYPVGGLCYYLSPPESMGAIFE
DPVHVVVYIIFMLESCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRDTSMVHELN
RYIPTAAAFGGLCIGALSVLADFLGAIGSGTGILLAVTIIYQYFEIFVKEQAEVGGMG
ALFF"
BASE COUNT 358 a 364 c 396 g 480 t
ORIGIN
1 aggatcgcgt cgggagccgg taccgaggcc cgagccgcgg gagtcgagcg aagacagcgc
61 cgaggccgcg gtttccccct gggcctcccc agcagcagcc atgggcatca aatttttaga
121 agttatcaaa ccattctgtg cagttctacc agaaattcag aaaccggaaa ggaaaatccc
181 actgtttgga atcatgtcat cagattctgc agatcctttc tactggatga gagttattct
241 ggcttccaat agaggaactt taatggaatt gggtatctcc ccaattgtaa catctggttt
301 gattatgcag ttgttagctg gagccaaaat cattgaagtt ggagatacac cgaaagatag
361 agctttattc aatggagccc agaaactgtt tggtatgatc attaccattg ggcaagccat
421 tgtgtatgtc atgacgggga tgtatgggga ccctgcagaa atgggtgccg gaatctgtct
481 cctgatcatc attcagttgt ttgttgctgg tttgattgtg ctgctgttag atgagctgct
541 acagaagggt tacggcttgg ggtctgggat ttccctcttt attgccacca acatctgtga
601 gaccattgtc tggaaggcct ttagtcccac taccattaac actggcagag gtactgagtt
661 tgagggtgca gtcatagctc tgttccattt gttggccacc aggacggaca aagtccgagc
721 tttacgggag gctttttatc ggcagaactt acccaatctc atgaacctca ttgctacagt
781 ttttgtgttt gctgttgtta tatatttcca aggatttcgc gttgatctgc ccattaagtc
841 ggcccgttac cgaggacagt acagcagcta ccccatcaaa ctcttctaca cctccaacat
901 ccccatcatc ctccagtcgg ccctggtgtc caacctgtat gttatttccc agatgctgtc
961 tgttcgattt agtggcaact ttttagtaaa tttactagga cagtgggccg atgtcagtgg
1021 gggaggaccc gcacgttctt acccagttgg aggcctttgt tactatcttt ctcctcctga
1081 gtccatgggc gccatctttg aggatcctgt ccatgtcgtt gtttatatca tcttcatgtt
1141 ggagtcatgt gcattcttct ctaagacatg gattgaagtg tctggttcct cagccaaaga
1201 tgtagctaaa cagctgaaag aacagcagat ggtaatgagg ggccaccgag atacctctat
1261 ggttcatgag cttaataggt acatccccac cgcagctgcg tttggcggtt tgtgcattgg
1321 cgccctgtca gtgctggctg acttcctggg ggccattgga tctggcactg gaattctgct
1381 agcagtcact attatttacc agtattttga aatatttgtt aaagaacagg ccgaagttgg
1441 tgggatgggt gctttgtttt tctaaatgtt caaatatttc attttgtgcg tgtgaaaggg
1501 aaaacacttt gacggatcgt ttttgtcaga tgacactggt ggctcccctt ttctcccctc
1561 acagtttctt gtttcgagtg ctgactgacc cgtttctg
//