LOCUS AK301777 2512 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ50052 complete cds, highly similar to Homo
sapiens laminin, beta 4 (LAMB4), mRNA.
ACCESSION AK301777
VERSION AK301777.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2512)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2512
/clone="TESTI2006141"
/clone_lib="TESTI2"
/db_xref="H-InvDB:HIT000496401"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="testis"
CDS 170..2248
/codon_start=1
/note="highly similar to Homo sapiens laminin, beta 4
(LAMB4), mRNA"
/protein_id="BAG63235.1"
/transl_table=1
/translation="MECPPGGGACLCDPVTGACPCLPNVTGLACDRCADGYWNLVPGR
GCQSCDCDPRTSQSSHCDQLTGQCPCKLGYGGKRCSECQENYYGDPPGRCIPCDCNRA
GTQKPICDPDTGMCRCREGVSGQRCDRCARGHSQEFPTCLQCHLCFDQWDHTISSLSK
AVQGLMRLAANMEDKRETLPVCEADFKDLIGNVSEIERILKHPVFPSGKFLKVKDYHD
SVRRQIMQLNEQLKAVYEFQDLKDTIERAKNEADLLLEDLQEEIDLQSSVLNASIADS
SENIKKYYHISSSAEKKINETSSTINTSANTRNDLLTILDTLTSKGNLSLERLKQIKI
PDIQILNEKVCGDPGNVPCVPLPCGGALCTGRKGHRKCRSPGCHGSLTLSTNALQKAQ
EAKSIIRNLDKQVRGLKNQIESISEQAEVSKNNALQLREKLGNIRNQSDSEEENINLF
IKKVKNFLLEENVPPEDIEKVANGVLDIHLPIPSQNLTDELVKIQKHMQLCEDYRTDE
NRLNEEADGAQKLLVKAKAAEKAANILLNLDKTLNQLQQAQITQGRANSTITQLTANI
TKIKKNVLQAENQTREMKSELELAKQRSGLEDGLSLLQTKLQRHQYHAVNAKVQAESA
QHQAGSLEKEFVELKKQYAILQRKTSTTGLTKETLGKVKQLKDAAEKLAGDTEAKIRR
ITGCFQNSAR"
BASE COUNT 803 a 543 c 609 g 557 t
ORIGIN
1 attagatgta accgatccag agtcctgcag ccgggtaaca ggggagtgcc ttcgatgttt
61 gcacaacact cagggcgcaa actgccagct ctgcaaacca ggtcactatg gatcagccct
121 caatcagacc tgcagaagat gctcctgcca tgcttccggc gtgagtccca tggagtgtcc
181 ccctggtggg ggagcttgcc tctgtgaccc tgtcactggt gcatgtcctt gtctgccgaa
241 tgtcacaggc ctggcctgtg accgttgtgc tgatggatac tggaatctgg tccctggcag
301 aggatgtcag tcatgtgact gtgaccctag gacctctcaa agtagccact gtgaccagct
361 tacaggccag tgtccgtgta aattaggtta cggcgggaaa cgttgcagtg agtgccagga
421 aaattattat ggtgatccac ctgggcgatg cattccatgt gattgtaaca gggcaggtac
481 ccagaagccc atctgtgatc cagacacagg catgtgccgc tgccgggagg gtgtcagcgg
541 ccagagatgt gatcgctgtg cccggggaca cagccaggaa ttccctactt gtcttcaatg
601 tcacttgtgc tttgatcagt gggaccacac catttcttcc ctctccaaag cggtgcaagg
661 gttaatgaga ctggctgcta acatggaaga taaaagagag accctgcctg tctgtgaggc
721 agacttcaaa gacctcatag ggaacgtgtc tgaaatagaa aggattttga aacatcctgt
781 tttcccatct gggaaattct taaaagtcaa ggattatcat gactctgtta gaagacaaat
841 catgcagcta aatgaacaac tgaaagcagt gtatgaattt caagatctga aagatacaat
901 agaaagagca aagaatgaag cagacctctt acttgaagac cttcaggaag aaattgattt
961 gcaatccagt gtccttaatg caagcattgc ggactcctca gaaaacatca agaaatatta
1021 tcacatatca tcatctgctg aaaagaaaat taatgaaact agttccacca ttaatacctc
1081 tgcaaataca aggaatgact tacttaccat cttagataca ctaacctcaa aaggaaactt
1141 gtcattggaa agattaaagc agattaagat accagatatc caaatattga atgaaaaggt
1201 gtgcggagat ccaggaaatg tgccatgtgt gcccttgccc tgtggcggtg ctctctgcac
1261 gggccggaag gggcacagga agtgtaggag tcccggctgt cacggctccc tgaccctctc
1321 aacgaatgcc ctccaaaaag cccaggaagc aaaatccatt attcgtaatt tggacaaaca
1381 ggttcgtggg ttgaaaaatc agatcgaaag tataagtgaa caggcagaag tctccaaaaa
1441 caatgcctta cagctgaggg aaaaactggg aaatataaga aaccaaagtg actctgaaga
1501 agaaaacatc aatcttttca tcaaaaaagt gaaaaacttt ttgttagagg aaaacgtgcc
1561 tccagaagac atcgagaagg ttgcgaatgg tgtgcttgac attcacctac caattccatc
1621 ccaaaatcta accgatgaac ttgtcaaaat acagaaacat atgcaactct gtgaggatta
1681 caggacagat gaaaacaggt taaatgaaga agcagatgga gcccaaaagc ttttggtgaa
1741 ggccaaagca gctgagaaag cagcaaatat tctattaaat cttgacaaaa cattgaacca
1801 gttacaacaa gctcaaatca ctcaaggacg ggcaaactct accattacac agctgactgc
1861 caatataaca aaaataaaaa agaatgtgct gcaggctgaa aatcaaacca gggaaatgaa
1921 gagtgagctg gagttagcaa agcagcgatc agggctggag gatggacttt ccctgctgca
1981 gaccaagttg caaaggcatc aataccacgc tgtcaatgcg aaagttcagg ctgaatctgc
2041 ccaacaccag gctgggagtc ttgagaagga atttgttgag ctgaaaaaac aatatgctat
2101 tctccaacgt aagacaagca ctacaggact aacaaaggag acattaggaa aagttaaaca
2161 gctaaaagat gcggcagaaa aattggctgg agatacagag gccaagataa gaagaataac
2221 aggctgtttt cagaactcag caagatagaa aaatgagaca ttgatttggc tgtcttcccc
2281 atgctgccac ttgagaaggg gcggaaccac aaggctgtgc ctgttcctct ctgttaggaa
2341 gaggagcccc tggatcccag tatggagccc ttggcagcaa caatgttgag agagtgggga
2401 atggactcta gtcttcagcc agggtccttc cacagaactt ggtgtcagtc ggggtcccag
2461 caagaaacag aaagcatgcg tgggcataat tttggataaa gtatttagaa gt
//