LOCUS AK301576 2350 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ58546 complete cds, highly similar to Homo
sapiens sphingomyelin phosphodiesterase 4, neutral membrane,
transcript variant 1, mRNA.
ACCESSION AK301576
VERSION AK301576.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2350)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2350
/clone="TBAES2004698"
/clone_lib="TBAES2"
/db_xref="H-InvDB:HIT000496200"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="tumor tissue"
/organism="Homo sapiens"
/tissue_type="breast, tumor tissue"
CDS 297..1889
/codon_start=1
/note="highly similar to Homo sapiens sphingomyelin
phosphodiesterase 4, neutral membrane, transcript variant
1, mRNA"
/protein_id="BAG63069.1"
/transl_table=1
/translation="MWLHHYSLEMYQKMQSPHAKESFTPTEEHVLVVRLLLKHLHAFA
NSLKPEQASPSAHSHATSPLEEFKRAAVPRFVQQKLYLFLQHCFGHWPLDASFRAVLE
MWLSYLQPWRYAPDKQAPGSDSQPRCVSEKWAPFVQENLLMYTKLFVGFLNRALRTDL
VSPKHALMVFRVAKVFAQPNLAEMIQKGEQLFLEPELVIPHRQHRLFTAPTFTGSFLS
PWPPAVTDASFKVKSHVYSLEGQDCKYTPMFGPEARTLVLRLAQLITQAKHTAKSISD
QCAESPAGHSFLSWLGFSSMDTNGSYTANDLDEMGQDSVRKTDEYLEKALEYLRQIFR
LSEAQLRQFTLALGTTQDENGKKQLPDCIVGEDGLILTPLGRYQIINGLRRFEIEYQG
DPELQPIRSYEIASLVRTLFRLSSAINHRFAGQMAALCSRDDFLGSFCRYHLTEPGLA
SRHLLSPVGRRQVAGHTRGPRLSLRFLGSYRTLVSLLLAFFVASLFCVGPLPCTLLLT
LGYVLYASAMTLLTERGKLHQP"
BASE COUNT 454 a 771 c 658 g 467 t
ORIGIN
1 gactttcggc gccgtggcgg aatggcggct tccatctctg aggcgagcga cgctatggat
61 cccacagtgg tttgctaaga aggccatttt caactctcca ctggaggctg ctatggcgtt
121 ccctcacctg cagcagccca gctttctact ggacaccagc catacccttt gcttcctatg
181 gcctccacca cactagcctc ctaaagcgac acatctctca tcagacatct gtgaatgcag
241 accccgcctc ccacgagatc tggaggtcag aaactctgct ccaggttttt gttgaaatgt
301 ggcttcatca ctattccttg gagatgtatc aaaaaatgca gtcccctcat gccaaggagt
361 cgttcacgcc tactgaggag catgtgttgg tggtgcgcct gctgctgaag cacctgcacg
421 cctttgccaa cagcctgaag ccagagcagg cctcaccctc cgcccactcc cacgccacca
481 gccccctgga ggagttcaaa cgggctgctg tcccgaggtt cgtccagcag aaactctacc
541 tcttcttgca gcattgcttt ggccactggc ccctggacgc atcgttcaga gctgtcctgg
601 agatgtggct gagctacctg cagccgtggc ggtacgcgcc tgacaagcag gctccgggca
661 gcgactccca gccccggtgt gtgtcggaga aatgggcacc ctttgtccag gagaacctgc
721 tgatgtacac caagttgttt gtgggctttc tgaaccgcgc gctccgcaca gacctggtca
781 gccccaagca cgcgctcatg gtgttccgag tggccaaagt ctttgcccag cccaacctgg
841 ctgagatgat tcagaaaggt gagcagctat tcctggagcc agagctggtc atcccccacc
901 gccagcaccg actcttcacg gcccccacat tcactgggag cttcctgtca ccctggccac
961 cagcggtcac tgatgcctcc ttcaaggtga agagccacgt ctacagcctg gagggccagg
1021 actgcaagta caccccgatg tttgggcccg aggcccgcac cctggtcctg cgcctcgctc
1081 agctcatcac acaggccaaa cacacagcca agtccatctc cgaccagtgt gcggagagcc
1141 cggctggcca ctccttcctc tcatggctgg gctttagctc catggacacc aatggctcct
1201 acacagccaa cgacctggac gagatggggc aagacagtgt ccggaagaca gatgaatacc
1261 tggagaaggc cctggagtac ctgcgccaga tattccggct cagcgaagcg cagctcaggc
1321 agttcacact cgccttgggc accacccagg atgagaatgg aaaaaagcaa ctccccgact
1381 gcatcgtggg tgaggacgga ctcatcctta cgcccctggg gcggtaccag atcatcaatg
1441 ggctgcgaag gtttgaaatt gagtaccagg gggacccgga gctgcagccc atccggagct
1501 atgagatcgc cagcttggtc cgcacactct ttaggctgtc gtctgccatc aaccacagat
1561 ttgcaggaca gatggcggct ctgtgttccc gggatgactt cctcggcagc ttctgtcgct
1621 accacctcac agaacctggg ctggccagca ggcacctgct gagccctgtg gggcggaggc
1681 aggtggccgg ccacacccgc ggccccaggc tcagcctgcg cttcctgggc agttaccgga
1741 cgctggtctc gctgctgctg gccttcttcg tggcctctct gttctgcgtc gggcccctcc
1801 catgcacgct gctgctcacc ctgggctatg tcctctacgc ctctgccatg acactgctga
1861 ccgagcgggg gaagctgcac cagccctgaa ggtgtcagct gccttcagag caggctggag
1921 ggatttgcca cacagcccca cccttgggct gagaggacct gggaagcccc tccaggaggg
1981 aacacggtca tcctcgggct tctggagcgg ggttcctgca gccgcagagg catctggagg
2041 aaacgcaacc aagaaaggaa ggcaggtggg ccccagcaaa ggagtagctg ccagggctca
2101 acagctacgc tctgtgacag cgcagagctc agcgccggcc tttccctccc tccgccaagg
2161 actcacggcc aagccagctc tcggggcctt ttttccagtg cccatttggc tactctgctg
2221 caccaagctt gggagccagc ctgccaacag ccacctgggc ctggcctccc cactggctgg
2281 ccttgaggtt ggcagagtgg gttgtggcgc ttcctctctc tgtgtgggac caggacagtg
2341 gcttaagtct
//