LOCUS AK301536 1978 bp mRNA linear HUM 31-JUL-2008
DEFINITION Homo sapiens cDNA FLJ59773 complete cds, highly similar to 72 kDa
type IV collagenase precursor (EC 3.4.24.24).
ACCESSION AK301536
VERSION AK301536.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1978)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1978
/cell_type="mesenchymal stem cells"
/clone="T1ESE2002722"
/clone_lib="T1ESE2"
/db_xref="H-InvDB:HIT000496160"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from mesenchymal stem cells treated with
trichostatin and 5-azacytidine."
/organism="Homo sapiens"
CDS 141..1895
/codon_start=1
/note="highly similar to 72 kDa type IV collagenase
precursor (EC 3.4.24.24)"
/protein_id="BAG63035.1"
/transl_table=1
/translation="MQKFFGLPQTGDLDQNTIETMRKPRCGNPDVANYNFFPRKPKWD
KNQITYRIIGYTPDLDPETVDDAFARAFQVWSDVTPLRFSRIHDGEADIMINFGRWEH
GDGYPFDGKDGLLAHAFAPGTGVGGDSHFDDDELWTLGEGQVVRVKYGNADGEYCKFP
FLFNGKEYNSCTDTGRSDGFLWCSTTYNFEKDGKYGFCPHEALFTMGGNAEGQPCKFP
FRFQGTSYDSCTTEGRTDGYRWCGTTEDYDRDKKYGFCPETAMSTVGGNSEGAPCVFP
FTFLGNKYESCTSAGRSDGKMWCATTANYDDDRKWGFCPDQGYSLFLVAAHEFGHAMG
LEHSQDPGALMAPIYTYTKNFRLSQDDIKGIQELYGASPDIDLGTGPTPTLGPVTPEI
CKQDIVFDGIAQIRGEIFFFKDRFIWRTVTPRDKPMGPLLVATFWPELPEKIDAVYEA
PQEEKAVFFAGNEYWIYSASTLERGYPKPLTSLGLPPDVQRVDAAFNWSKNKKTYIFA
GDKFWRYNEVKKKMDPGFPKLIADAWNAIPDNLDAVVDLQGGGHSYFFKGAYYLKLEN
QSLKSVKFGSIKSDWLGC"
BASE COUNT 450 a 567 c 556 g 405 t
ORIGIN
1 agatgttgtc ttgtgagcgt gcgcgcgcct ggctggaggg gcactgagcc tggccgcagt
61 gttgccaata cctgaacacc ttctatggct gccccaagga gagctgcaac ctgtttgtgc
121 tgaaggacac actaaagaag atgcagaagt tctttggact gccccagaca ggtgatcttg
181 accagaatac catcgagacc atgcggaagc cacgctgcgg caacccagat gtggccaact
241 acaacttctt ccctcgcaag cccaagtggg acaagaacca gatcacatac aggatcattg
301 gctacacacc tgatctggac ccagagacag tggatgatgc ctttgctcgt gccttccaag
361 tctggagcga tgtgacccca ctgcggtttt ctcgaatcca tgatggagag gcagacatca
421 tgatcaactt tggccgctgg gagcatggcg atggataccc ctttgacggt aaggacggac
481 tcctggctca tgccttcgcc ccaggcactg gtgttggggg agactcccat tttgatgacg
541 atgagctatg gaccttggga gaaggccaag tggtccgtgt gaagtatggg aacgccgatg
601 gggagtactg caagttcccc ttcttgttca atggcaagga gtacaacagc tgcactgata
661 ccggccgcag cgatggcttc ctctggtgct ccaccaccta caactttgag aaggatggca
721 agtacggctt ctgtccccat gaagccctgt tcaccatggg cggcaacgct gaaggacagc
781 cctgcaagtt tccattccgc ttccagggca catcctatga cagctgcacc actgagggcc
841 gcacggatgg ctaccgctgg tgcggcacca ctgaggacta cgaccgcgac aagaagtatg
901 gcttctgccc tgagaccgcc atgtccactg ttggtgggaa ctcagaaggt gccccctgtg
961 tcttcccctt cactttcctg ggcaacaaat atgagagctg caccagcgcc ggccgcagtg
1021 acggaaagat gtggtgtgcg accacagcca actacgatga tgaccgcaag tggggcttct
1081 gccctgacca agggtacagc ctgttcctcg tggcagccca cgagtttggc cacgccatgg
1141 ggctggagca ctcccaagac cctggggccc tgatggcacc catttacacc tacaccaaga
1201 acttccgtct gtcccaggat gacatcaagg gcattcagga gctctatggg gcctctcctg
1261 acattgacct tggcaccggc cccaccccca cgctgggccc tgtcactcct gagatctgca
1321 aacaggacat tgtatttgat ggcatcgctc agatccgtgg tgagatcttc ttcttcaagg
1381 accggttcat ttggcggact gtgacgccac gtgacaagcc catggggccc ctgctggtgg
1441 ccacattctg gcctgagctc ccggaaaaga ttgatgcggt atacgaggcc ccacaggagg
1501 agaaggctgt gttctttgca gggaatgaat actggatcta ctcagccagc accctggagc
1561 gagggtaccc caagccactg accagcctgg gactgccccc tgatgtccag cgagtggatg
1621 ccgcctttaa ctggagcaaa aacaagaaga catacatctt tgctggagac aaattctgga
1681 gatacaatga ggtgaagaag aaaatggatc ctggcttccc caagctcatc gcagatgcct
1741 ggaatgccat ccccgataac ctggatgccg tcgtggacct gcagggcggc ggtcacagct
1801 acttcttcaa gggtgcctat tacctgaagc tggagaacca aagtctgaag agcgtgaagt
1861 ttggaagcat caaatccgac tggctaggct gctgagctgg ccctggctcc cacaggccct
1921 tcctctccac tgccttcgat acaccgggcc tggagaacta gagaaggacg cggagggg
//