LOCUS AK301532 979 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ53748 complete cds, moderately similar to
Peroxisomal membrane protein 4.
ACCESSION AK301532
VERSION AK301532.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 979)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..979
/cell_type="mesenchymal stem cells"
/clone="T1ESE2000488"
/clone_lib="T1ESE2"
/db_xref="H-InvDB:HIT000496156"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from mesenchymal stem cells treated with
trichostatin and 5-azacytidine."
/organism="Homo sapiens"
CDS 104..538
/codon_start=1
/note="moderately similar to Peroxisomal membrane protein
4"
/protein_id="BAG63033.1"
/transl_table=1
/translation="MAAPPQLRALLVVVNALLRKRRYHAALAVLKGFRNGAVYGAKIR
APHALVMTFLFRNGRGTCVCAASRRSCGPYCRPHISTPGTWHGLCSPTRVSVPCSPTY
KARPTQHTHSWRPSSGVSWCLEKTITSTARSTCTCCHASCLP"
BASE COUNT 194 a 302 c 264 g 219 t
ORIGIN
1 acgagtcggc ggcgctaccg aggggctgtg ggcgcgcagc tggaacctcc ggctgtcagt
61 gcgcttacag ttcctaaccc cgaccctgcg cgcagcccgc actatggcag ccccgccgca
121 gctaagggct ctgctcgtag tcgtcaacgc actgctgcgc aagcgccgct accacgctgc
181 gttggccgtg cttaagggct tccggaacgg ggctgtctat ggagccaaaa tccgggcccc
241 tcacgcgctg gtcatgacct ttctcttccg gaatggcagg ggcacctgtg tttgtgcagc
301 ctccaggaga agctgtgggc catactgcag gccacatata tccactcctg gaacctggca
361 cggtttgtgt tcacctacaa gggtctccgt gccctgcagt cctacataca aggcaagacc
421 tacccagcac acgcattcct ggcggccttc ctcgggggta tcctggtgtt tggagaaaac
481 aataacatca acagccagat caacatgtac ctgttgtcac gcgtcctgtt tgccctgagc
541 cgcctggctg tagagaaggg ctacatccct gaacccaggt gggacccgtt cccgctgctc
601 actgcggtgg tgtgggggct ggtgctgtgg ctctttgagt atcaccgatc caccctgcag
661 ccctcgctgc agtcctccat gacctacctc tatgaggaca gcaatgtatg gcacgacatc
721 tcagacttcc tcatctataa caagagccgt ccctccaatt aatgcagccc tgaggtgtct
781 ggctgtggct caagatttgg ccccatgcag accctcccaa aggatactgc cttctcaaga
841 tcataggcct cagactccaa ctggtgttat cccagggttc cgtttgctga agtaacaaca
901 ctgattttaa aatcccagtg ggtacctttg tatggtggca caagtggccg aatcaggctg
961 aggaatctac ggcttggtt
//