LOCUS AK301431 2423 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ61430 complete cds, highly similar to
ATP-dependent RNA helicase DDX50 (EC 3.6.1.-).
ACCESSION AK301431
VERSION AK301431.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2423)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2423
/clone="SYNOV2019114"
/clone_lib="SYNOV2"
/db_xref="H-InvDB:HIT000496055"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="synovial membrane tissue from rheumatoid
arthritis"
CDS 72..2207
/codon_start=1
/note="highly similar to ATP-dependent RNA helicase DDX50
(EC 3.6.1.-)"
/protein_id="BAG62959.1"
/transl_table=1
/translation="MPGKLLWGDIMELEAPLEESESQKKERQKSDRRKSRHHYDSDEK
SETRENGVTDDLDAPKAKKSKMKEKLNGDTEEGFNRLSDEFSKSHKSRRKDLPNGDID
EYEKKSKRVSSLDTSTHKSSDNKLEDTLTREQKEGAFSNFPISEETIKLLKGRGVTYL
FPIQVKTFGPVYEGKDLIAQARTGTGKTFSFAIPLIERLQRNQETIKKSRSPKVLVLA
PTRELANQVAKDFKDITRKLSVAWFYGGTSYQSQINHIRNGIDILVGTPGRIKDHLQS
GRLDLSKLRHVVLDEVDQMLDLGFAEQVEDIIHESYKTDSEDNPQTLLFSATCPQWVY
KVAKKYMKSRYEQVDLVGKMTQKAATTVEHLAIQCHWSQRPAVIGDVLQVYSGSEGRA
IIFCETKKNVTEMAMNPHIKQNAQCLHGDIAQSQREITLKGFREGSFKVLVATNVAAR
GLDIPEVDLVIQSSPPQDVESYIHRSGRTGRAGRTGICICFYQPRERGQLRYVEQKAG
ITFKRVGVPSTMDLVKSKSMDAIRSLASVSYAAVDFFRPSAQRLIEEKGAVDALAAAL
AHISGASSFEPRSLITSDKGFVTMTLESLEEIQDVSCAWKELNRKLSSNAVSQITRMC
LLKGNMGVCFDVPTTESERLQAEWHDSDWILSVPAKLPEIEEYYDGNTSSNSRQSRQG
SRSGSRQDGRRRSGNRNRSRSGGHKRSFD"
BASE COUNT 781 a 444 c 557 g 641 t
ORIGIN
1 ctttttcacg ctgtcgctgc ccgtaggtgg ttgtggccac tgtgcccgga gggaggcggc
61 ggtggccagt aatgcctggg aaactcctct ggggggacat tatggagctg gaagcaccct
121 tggaggagtc cgagagccag aagaaggaga ggcaaaagag tgacagaagg aagtcaaggc
181 accattatga ctcggatgag aaatcagaaa caagagaaaa tggtgttaca gatgacctgg
241 atgctcccaa ggccaaaaaa tctaaaatga aagagaagct aaatggagac actgaagaag
301 gatttaatag actttcagat gaattctcca aatctcataa gtcaagaaga aaagatctac
361 caaatggaga tatagatgaa tatgaaaaaa aatcaaagcg agtatcatct ttagatactt
421 ctactcataa atcaagtgat aataaactag aggatacctt aacacgtgaa cagaaagaag
481 gagccttctc caattttcct atttctgaag agactataaa gcttctgaaa ggtcgagggg
541 taacatatct ctttcctatt caagttaaga cctttggtcc tgtatatgaa ggaaaagatt
601 taatagctca agcacggaca ggaacaggaa agacattctc ttttgccatc cccttaattg
661 aaagactcca aagaaatcaa gaaacaatta aaaaaagccg ctcaccaaag gtacttgttt
721 tggctccaac aagggaactg gcaaaccaag tagccaaaga cttcaaagat ataactagga
781 aactcagcgt ggcgtggttt tatggtggaa catcatatca aagccaaatt aatcatattc
841 gaaatggtat tgacatcttg gttggaacac ctggtcgtat caaagaccat ctgcagagtg
901 gccgattgga tctttctaaa ctgcgacatg ttgtgcttga tgaagtggat cagatgttag
961 atttaggttt cgctgaacaa gttgaagata ttattcatga atcctacaaa actgattctg
1021 aagacaatcc tcagacttta cttttttctg caacttgccc acagtgggta tacaaagttg
1081 caaaaaaata catgaaatcc agatatgaac aggttgacct tgttggaaaa atgactcaaa
1141 aggctgcaac tactgtggaa catttggcca tccagtgtca ttggtctcag aggccagcag
1201 ttattggaga tgtccttcaa gtctacagtg ggtctgaagg gagggctatt attttctgtg
1261 agaccaagaa gaatgtaact gaaatggcca tgaatccaca cataaaacag aatgcccagt
1321 gtttacatgg ggacattgca cagtcacaaa gagaaattac actaaaaggc ttcagagaag
1381 gtagttttaa agttttggtg gcaaccaatg tggctgcccg tggtttggac attcctgaag
1441 ttgacctggt gattcaaagt tctcctcctc aggatgttga gtcctatatc catcgctctg
1501 gacgcacagg tagagctgga cggacaggga tttgtatatg tttttatcaa ccaagagaaa
1561 gaggtcaact aagatatgtg gaacaaaaag caggaattac ttttaaacgt gtaggtgttc
1621 cttctacaat ggatttagtt aaatctaaaa gcatggatgc catcaggtct ctggcttccg
1681 tttcttatgc tgctgttgat tttttccgac catcagctca gagactgata gaagagaaag
1741 gtgcagtgga tgcattggct gcagctttag cccacatttc tggtgcatca agctttgaac
1801 cacgatcttt gatcacctct gataaggggt ttgtgaccat gactctggaa agcctagagg
1861 aaatacagga tgtcagctgt gcttggaaag aacttaacag aaagctgagt agtaatgcag
1921 tgtctcagat taccagaatg tgcctcctga aagggaatat gggtgtttgc tttgatgttc
1981 ctacaactga gtcagaaagg ttacaggcag agtggcatga ttccgactgg atactctcag
2041 tgccagccaa attacctgaa attgaagaat attatgatgg aaacacatct tctaattcca
2101 gacagagtcg acaaggaagt cgctcaggaa gtcgacaaga tggtagaaga cgaagtggga
2161 atagaaatcg atcaagaagt gggggccaca aacggagttt tgactgagta tttgatagtt
2221 aatctaccag tgtgagcttg cctatttctg cctaatcatg tacattatcc accaaaaatt
2281 aggtcatcat agttgaggta tgtgtctgct atttgcaaag aagttggtcg tattttttta
2341 aaaagtattt cacaagaatg gaacaaatct acttatccag ttataccttt gaataaaaaa
2401 acctcctttt attgtctctt ttc
//