LOCUS AK301294 2258 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ50253 complete cds, highly similar to Heat
shock 70 kDa protein 12B.
ACCESSION AK301294
VERSION AK301294.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2258)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2258
/clone="STOMA2002026"
/clone_lib="STOMA2"
/db_xref="H-InvDB:HIT000495918"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="stomach"
CDS 269..2071
/codon_start=1
/note="highly similar to Heat shock 70 kDa protein 12B"
/protein_id="BAG62852.1"
/transl_table=1
/translation="MMRKWEGGYPGVAHQKTPTCLLLTPEGAFHSFGYTARDYYHDLD
PEEARDWLYFEKFKMKIHSATDLTLKTQLEAVNGKTMPALEVFAHALRFFREHALQEL
REQSPSLPEKDTVRWVLTVPAIWKQPAKQFMREAAYLAGLVSRENAEQLLIALEPEAA
SVYCRKLRLHQLLDLSGRAPGGGRLGERRSIDSSFRQAREQLRRSRHSRTFLVESGVG
ELWAEMQAGDRYVVADCGGGTVDLTVHQLEQPHGTLKELYKASGGPYGAVGVDLAFEQ
LLCRIFGEDFIATFKRQRPAAWVDLTIAFEARKRTAGPHRAGALNISLPFSFIDFYRK
QRGHNVETALRRSSVNFVKWSSQGMLRMSCEAMNELFQPTVSGIIQHIEALLARPEVQ
GVKLLFLVGGFAESAVLQHAVQAALGARGLRVVVPHDVGLTILKGAVLLGQAPGVVRV
RRSPLTYGVGVLNRFVPGRHPPEKLLVRDGRRWCTDVFERFVAAEQSVALGEEVRRSY
CPARPGQRRVLINLYCCAAEDARFITDPGVRKCGALSLELEPADCGQDTAGAPPGRRE
IRAAMQFGDTEIKVTAVDVSTNRSVRASIDFLSN"
BASE COUNT 373 a 738 c 769 g 378 t
ORIGIN
1 gggctgcggc cgccgcagcg ggcacggcca acgagctgcg ggcccgggat cgcggcggct
61 ggacggggct ggagctgtcg ggagggcgga gctacagggc ctgcaaggat gttggctgtc
121 ccggagatgg gcctgcaggg gctgtacatc gaaacccgag gtccgagccc cccagcaggc
181 ctccttctct gtggtggtgg ccattgactt cggcaccacg tctagtggct atgctttcag
241 ctttgccagt gaccctgagg ccatccacat gatgaggaaa tgggagggcg gatacccggg
301 cgtggcccac cagaagaccc cgacctgcct gctgctgact ccggagggcg ccttccacag
361 ctttggctac accgcccgcg attactacca tgacctggac cccgaagagg cgcgggactg
421 gctctacttc gagaagttca agatgaagat ccacagcgcc acggatctca ccttgaagac
481 ccagctagag gcagtaaatg gaaagacgat gcccgccctg gaggtgttcg cccatgccct
541 gcgcttcttc agggagcacg cccttcagga gctgagggag cagagcccat cgctgccaga
601 gaaggacact gtgcgctggg tgttgacggt gcctgccatc tggaaacagc cagccaagca
661 gttcatgcgg gaggctgcct acctggctgg actagtgtcc cgagagaatg cagagcagct
721 actcatcgcc ctggagcccg aggccgcctc ggtatactgc cgcaagctgc gcctgcacca
781 gctcctggac ctgagtggcc gggccccagg tggtgggcgc ctgggtgagc gccgctccat
841 cgactccagc ttccgtcagg ctcgggagca gctgcgaagg tcccgccaca gccgcacgtt
901 cctggtggag tcaggcgtag gagagctgtg ggcagagatg caagcaggag accgctacgt
961 ggtggccgac tgcggcggag gcaccgtgga cctgacggtg caccagctgg agcagcccca
1021 tggcaccctc aaggagctct acaaggcatc tgggggccct tatggcgcgg tgggcgtgga
1081 cctggccttc gagcagctgc tgtgccgcat cttcggcgag gacttcatcg ccaccttcaa
1141 aaggcaacgg ccggcagcct gggtagatct gaccatcgcc ttcgaggctc gcaagcgcac
1201 tgctggccca caccgtgcag gggcgctcaa catctcgctg cccttctcct tcattgactt
1261 ctaccgcaag cagcggggcc acaacgtgga gaccgctctg cgcaggagca gcgtgaactt
1321 cgtgaagtgg tcctcacagg ggatgctccg aatgtcttgt gaagccatga acgagctctt
1381 tcagcccacc gtcagcggga tcatccagca catagaggcc ctgctggcac ggccggaggt
1441 gcagggtgtg aagctgctgt tcctagtggg cggcttcgcc gaatcagcgg tgctgcagca
1501 cgcggtgcag gcggcgctgg gcgcccgcgg tctgcgtgtc gtggtcccgc acgacgtggg
1561 cctcaccatc ctcaaaggcg cggtgctgct cggccaggcg ccgggcgtgg tgcgggtccg
1621 ccgctcgccg ctcacctatg gcgtgggcgt gctcaaccgc tttgtgcctg ggcgccaccc
1681 gcccgaaaag ctgctggttc gcgacggccg ccgctggtgc accgacgtct tcgagcgctt
1741 cgtggccgcc gagcagtcgg tggccctggg cgaggaggtg cggcgcagct actgcccggc
1801 gcgtcccggc cagcggcgcg tactcatcaa cctgtactgc tgcgcggcag aggatgcgcg
1861 cttcatcacc gaccccggcg tgcgcaaatg tggcgcgctc agcctcgagc ttgagcccgc
1921 cgactgcggc caggacaccg ccggcgcgcc tcccggccgc cgcgagatcc gcgccgccat
1981 gcagtttggc gacaccgaaa ttaaggtcac cgccgtcgac gtcagcacca atcgctccgt
2041 gcgcgcgtcc atcgactttc tttccaactg agggcgcgcc ggcgcggtgc cagcgccgtc
2101 tgcccggccc cgccctcttt cggttcaggg gcctgcggag cgggttgggg cgggggaaac
2161 gatagttctg cagtctgcgc ctttccacgc cctccagccc cgggggagat aaggtcatgg
2221 gagagtgggt ggggacacac ccagagactg gctttggg
//