LOCUS AK301286 1072 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ60006 complete cds, highly similar to Pepsin
A precursor (EC 3.4.23.1).
ACCESSION AK301286
VERSION AK301286.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1072)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1072
/clone="STOMA2000861"
/clone_lib="STOMA2"
/db_xref="H-InvDB:HIT000495910"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="stomach"
CDS 53..901
/codon_start=1
/note="highly similar to Pepsin A precursor (EC 3.4.23.1)"
/protein_id="BAG62845.1"
/transl_table=1
/translation="MKWLLLLGLVALSECIMYKVPLIRKKSLRRTLSERGLLKDFLKK
HNLNPARKYFPQWKAPTLVDEQPLENYLDMEYFGTIGIGTPAQDFTVLFDTGSSNLWV
PSVYCSSLACTNHNRFNPEDSSTYQSTSETVSITYGISSLPDIVFTINGVQYPVPPSA
YILLVSQDLFSVYLSADDQSGSVVIFGGIDSSYYTGSLNWVPVTVEGYWQITVDSITM
NGEAIACAEGCQAIVDTGTSLLTGPTSPIANIQSDIGASENSDGDMVVSCSAISSLPD
IVFTIH"
BASE COUNT 250 a 348 c 257 g 217 t
ORIGIN
1 gcaccttcct cccgtcttgc cttctccctc gagttgggac ccgggaagaa ccatgaagtg
61 gctgctgctg ctgggtctgg tggcactctc tgagtgcatc atgtacaagg tccccctcat
121 cagaaagaag tccttgaggc gcaccctgtc cgagcgtggc ctgctgaagg acttcctgaa
181 gaagcacaac ctcaacccag ccagaaagta cttcccccag tggaaggctc ccaccctggt
241 agatgaacag cccctggaga actacctgga tatggagtac ttcggcacta tcggcatcgg
301 aactcctgcc caggatttca ctgtcctctt tgacaccggc tcctccaacc tgtgggtgcc
361 ctcagtctac tgctccagtc ttgcctgcac caaccacaac cgcttcaacc ctgaggattc
421 ttccacctac cagtccacca gcgagacagt ctccatcacc tacggcatca gcagcctgcc
481 cgacatcgtc ttcaccatca atggagtcca gtaccccgtg ccacccagtg cctacatcct
541 gctggtttct caggacctct tctctgtcta cctcagcgcc gatgaccaga gtggcagcgt
601 ggtgatcttt ggtggcattg actcttctta ctacactgga agtctgaact gggtgcctgt
661 taccgtcgag ggttactggc agatcaccgt ggacagcatc accatgaacg gagaggccat
721 cgcctgcgct gagggctgcc aggccattgt tgacaccggc acctctctgc tgaccggccc
781 aaccagcccc attgccaaca tccagagcga catcggagcc agcgagaact cagatggcga
841 catggtggtc agctgctcag ccatcagcag cctgcccgac atcgtcttca ccatccacta
901 gaggggttca cacagaatgt ggacacagag ttcccctctg cagacggaaa gtacacttcc
961 acgagctgaa gccaagaggc agaggcagac gaacatctgc cctagacagc ctccagagaa
1021 aaagaatata ttaaaaacaa atgcaggaat aagaactcgg atacagcccc ct
//