LOCUS AK301272 2886 bp mRNA linear HUM 31-JUL-2008
DEFINITION Homo sapiens cDNA FLJ55373 complete cds, highly similar to
Isoleucyl-tRNA synthetase, cytoplasmic (EC 6.1.1.5).
ACCESSION AK301272
VERSION AK301272.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2886)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2886
/clone="SPLEN2041114"
/clone_lib="SPLEN2"
/db_xref="H-InvDB:HIT000495896"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="spleen"
CDS 104..2422
/codon_start=1
/note="highly similar to Isoleucyl-tRNA synthetase,
cytoplasmic (EC 6.1.1.5)"
/protein_id="BAG62834.1"
/transl_table=1
/translation="MLQQVPENINFPAEEEKILEFWTEFNCFQECLKQSKHKPKFTFY
DGPPFATGLPHYGHILAGTIKDIVTRYAHQNYKDVQDPSVFVTFPLEEDETVSLVAWT
TTPWTLPSNLAVCVNPEMQYVKIKDVARGRLLILMEARLSALYKLESDYEILERFPGA
YLKGKKYNAYRFLIQNVLRLQKEEEIEFLYNENTVRESPNITDRWILSFMQSLIGFFE
TEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGMEDCVMALETLSSVLLSLC
RLMAPYTPFLTELMYQNLKVLIDPVSVQDKDTLSIHYLMLPRVREELIDKKTESAVSQ
MQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDPEALKDIKSLEKYIIEELNVRKVTLS
TDKNKYGIRLRAEPDHMVLGKRLKGAFKAVMTSIKQLSSEELEQFQKTGTIVVEGHEL
HDEDIRLMYTFDQATGGTAQFEAHSDAQALVLLDVTPDQSMVDEGMAREVINRIQKLR
KKCNLVPTDEITVYYKAKSEGTYLNSVIESHTEFIFTTIKAPLKPYPVSPSDKVLIQE
KTQLKGSELEITLTRGSSLPGPACAYVNLNICANGSEQGGVLLLENPKGDNRLDLLKL
KSVVTSIFGVKNTELAVFHDETEIQNQTDLLSLSGKTLCVTAGSAPSLINSSSTLLCQ
YINLQLLNAEPQECLMGTVGTLLLENPLGQNGLTHQGLLYEAAKVFGLRSRKLKLFLN
ETQTQEITEDIPVKTLNMKTVYVSVLPTTADF"
BASE COUNT 871 a 595 c 618 g 802 t
ORIGIN
1 atcacctccg tgcgcccggt tgcagcgtgg acgccggatg agttgctttt aggcttgctg
61 gcccgcgggg ctgtccaggc acgcgaggcc cctcagcaac aaaatgcttc aacaagttcc
121 agaaaacata aattttcctg ctgaagaaga gaaaatcttg gagttttgga ctgaatttaa
181 ttgttttcag gaatgcttaa agcaatcaaa acataaacca aaatttacct tctatgatgg
241 tcctcctttt gcaactggac tgcctcacta tggacatata cttgcgggta caattaaaga
301 tatagttaca agatatgctc accagaatta taaggatgtt caagatcctt cagtatttgt
361 aactttccct ttggaagaag atgaaactgt atctttagtt gcttggacaa ccactccctg
421 gactctacct agtaaccttg ctgtgtgtgt taatccagaa atgcaatatg tgaaaattaa
481 agatgttgcc agaggacgat tactcatttt aatggaagcc agattgtcag ccctctataa
541 attggagagt gactatgaga tccttgaaag atttcctggt gcctatctta aaggcaagaa
601 gtacaatgcc tatcgcttct taatccagaa cgttctgagg ctccagaagg aggaagaaat
661 agaatttctc tacaatgaga acacggttag agaaagcccc aacattacag accggtggat
721 cctgtccttc atgcagtctc tcattggctt ctttgagact gaaatggcag cttataggct
781 ttatactgtg gtgcctcgcc tggtcaagtt tgtagatatt ctgaccaatt ggtatgttag
841 aatgaaccgc agaagattaa agggtgaaaa tgggatggag gattgtgtca tggccctaga
901 aaccttgtct agtgttctgc tttctctttg cagacttatg gctccctaca caccttttct
961 cactgaattg atgtaccaga atctaaaggt gctgattgac cctgtttctg ttcaggacaa
1021 ggacacactc agcattcact acctcatgct gccccgtgtt cgagaagaat tgattgacaa
1081 gaaaacagag agtgcagtat ctcagatgca gtctgtgatt gaacttggaa gagtgatcag
1141 agaccgaaaa actattccca taaagtatcc tttgaaagaa attgtggtta tccatcaaga
1201 tccagaagct cttaaagata tcaagtcttt ggagaagtat atcattgagg aactcaatgt
1261 tcgaaaagtt acactgtcta cagataaaaa caagtatggc attcggctaa gggcagaacc
1321 agatcacatg gtcctgggga agcgtctgaa gggagccttt aaggcagtga tgacgtccat
1381 caagcagttg agcagtgagg agctggagca gttccagaag actgggacca ttgttgtgga
1441 aggccatgaa ttgcacgatg aagacatccg cctcatgtac acctttgatc aggccacagg
1501 tgggactgcg caatttgaag cacactcaga tgctcaggct ttggtcctct tagatgtcac
1561 tcctgaccag tcaatggtag acgaaggaat ggctcgggaa gtcatcaatc gcatacagaa
1621 acttcgcaaa aagtgcaatc tggttccaac tgatgaaatc acagtgtact ataaagcaaa
1681 gtctgaagga acatatctga atagtgttat tgaaagccac acagagttca tatttaccac
1741 cataaaggct cccttgaaac catatccagt ttctccatcg gataaagtcc ttattcaaga
1801 aaaaacacag ttgaagggat ctgaactgga aattacactc accagaggat cttcccttcc
1861 tggtcctgct tgtgcatatg tcaatcttaa catttgtgca aatggcagtg aacaaggtgg
1921 agtattgctc ctggaaaatc caaaaggtga caataggttg gaccttttaa agctgaagag
1981 tgttgtcact agcatttttg gtgtgaaaaa tacagagctg gctgtcttcc atgatgaaac
2041 agaaatacaa aaccaaactg acttactgag tcttagtgga aaaacacttt gtgtgactgc
2101 aggatcggct ccctctctga tcaacagttc tagtactctt ctttgtcagt atatcaacct
2161 acagctcctg aatgcagagc cacaagagtg tttaatgggg acagtgggca ctctcctgct
2221 tgaaaaccca cttgggcaga atggactcac ccaccaaggt cttctgtatg aagcagccaa
2281 ggtgtttggc cttcggagca ggaagctaaa gctgtttctg aatgagaccc aaacgcagga
2341 aattacagaa gacatccccg tgaagacttt gaatatgaag actgtgtatg tttctgtgtt
2401 accaacaaca gcagacttct agcatgtact tatcaatgtt gttcggtcag cccttcccta
2461 attacaccta tcccctacac atacatgcac atagacacac acatgaacac actgaagata
2521 tttccttcag gtgtgtgtaa aatacgctgc ttggattgaa attcaaatgg gattgattag
2581 tcaagtaact tgagtcctca cagtaatctt cacacttaac cttagacacc tatgcagtca
2641 tgttgggagc aggttacaat gttacttcag cccacagttt atttctatac ttgagttctt
2701 aagtacagaa gatagaagtg atttaaatgg catagtatat atatcatttt ctggcctttt
2761 aaaatttatt tgagacctct tgatgaaatg gacatattat atatttctgc cacctggatt
2821 ttcctggata atttgatgga atattttaag tttcagtaaa tcagaacaat aaacaaactc
2881 agatat
//