LOCUS AK301234 1498 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ50030 complete cds, highly similar to Homo
sapiens calcium channel, voltage-dependent, alpha 2/delta subunit
4, transcript variant 3, mRNA.
ACCESSION AK301234
VERSION AK301234.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1498)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1498
/clone="SPLEN2033772"
/clone_lib="SPLEN2"
/db_xref="H-InvDB:HIT000495858"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="spleen"
CDS 83..886
/codon_start=1
/note="highly similar to Homo sapiens calcium channel,
voltage-dependent, alpha 2/delta subunit 4, transcript
variant 3, mRNA"
/protein_id="BAG62806.1"
/transl_table=1
/translation="MKLEFLQRKFWAATRQDLDCFVIDNNGFILISKRSRETGRFLGE
VDGAVLTQLLSMGVFSQVTMYDYQAMCKPSSHHHSAAQPLVSPISAFLTATRWLLQEL
VLFLLEWSVWGSWYDRGAEAKSVFHHSHKHKKQDPLQPCDTEYPVFVYQPAIREANGI
VECGPCQKVFVVQQIPNSNLLLLVTDPTCDCSIFPPVLQEATEVKYNASVKCDRMRSQ
KLRRRPDSCHAFHPEENAQDCGGASDTSASPPLLLLPVCAWGLLPQLLR"
BASE COUNT 317 a 449 c 410 g 322 t
ORIGIN
1 aacttgactc tatttttttc tctccccgtc tcttccctgg ccttgccctc tctctttctg
61 ccctgtagcc gcgggcgtcc aaatgaagct ggaattcctc cagcgcaaat tctgggcggc
121 aacgcggcag gatctggact gcttcgtcat cgacaacaac gggttcattc tgatctccaa
181 gaggtcccga gagacgggaa gatttctggg ggaggtggat ggtgctgtcc tgacccagct
241 gctcagcatg ggggtgttca gccaagtgac tatgtatgac tatcaggcca tgtgcaaacc
301 ctcgagtcac caccacagtg cagcccagcc cctggtcagc ccaatttctg ccttcttgac
361 ggcgaccagg tggctgctgc aggagctggt gctgttcctg ctggagtgga gtgtctgggg
421 ctcctggtac gacagagggg ccgaggccaa aagtgtcttc catcactccc acaaacacaa
481 gaagcaggac ccgctgcagc cctgcgacac ggagtacccc gtgttcgtgt accagccggc
541 catccgggag gccaacggga tcgtggagtg cgggccctgc cagaaggtat ttgtggtgca
601 gcagattccc aacagtaacc tcctcctcct ggtgacagac cccacctgtg actgcagcat
661 cttcccacca gtgctgcagg aggcgacaga agtcaaatat aatgcctctg tcaaatgtga
721 ccggatgcgc tcccagaagc tccgccggcg accagactcc tgccacgcct tccatccgga
781 ggagaatgcc caggactgcg gcggcgcctc ggacacctca gcctcgccgc ccctactcct
841 gctgcctgtg tgtgcctggg ggctactgcc ccaactcctg cggtgacacc acccagcctg
901 acctgtgttt tggcaaggtg atccttccag agccatccca caaagtcagc actgacatgg
961 gatgcagcta actgcagttg ggtcgccccc aggccaacgc tcctctcaat cctgggctgg
1021 tggcccctgg ctccggagaa tgctggatgg aacaggaaac caatcacctg gcaccacttt
1081 caagatgctt catggtgccc ggtaccatct gccctaggtc tcaacatgag catacttctg
1141 acctaacctt cctgtctcct cttcgggaag ccagcgtgag ctcagcttgg accaagacaa
1201 aataatttag ttcttcctgt actccagagt ccagacccag ccaagaaagg gtcagttgtt
1261 tctgaccctt tctgtcggag tggtctctgg tagaacccaa ggacttctgg gtactgagaa
1321 gcagcagcag aatgaggcca aatgcagaaa tggggctaag gcaagaatat gccccaacta
1381 aagcatagat tccccaaagt gaggctcatg gtgggaggcc actcaccttc ctagctgctg
1441 ctcgaaaagg ttttgactgt gttggggtgg gggttgggta agggaatggt caagactg
//