LOCUS AK301212 2495 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ59445 complete cds, highly similar to
Peroxisomal multifunctional enzyme type 2.
ACCESSION AK301212
VERSION AK301212.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2495)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2495
/clone="SPLEN2029320"
/clone_lib="SPLEN2"
/db_xref="H-InvDB:HIT000495836"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="spleen"
CDS 80..2236
/codon_start=1
/note="highly similar to Peroxisomal multifunctional
enzyme type 2"
/protein_id="BAG62787.1"
/transl_table=1
/translation="MGSPLRFDGRVVLVTGAGAVNDLGGDFKGVGKGSLAADKVVEEI
RRRGGKAVANYDSVEEGEKVVKTALDAFGRIDVVVNNAGILRDHSFARISDEDWDIIH
RVHLRGSFQVTRAAWEHMKKQKYGRIIMTSSASGIYGNFGQANYSAAKLGLLGLANSL
AIEGRKSNIHCNTIAPNAGSRMTQTVMPEDLVEALKPEYVAPLVLWLCHESCEENGGL
FEVGAGWIGKLRWERTLGAIVRQKNHPMTPEAVKANWKKICDFENASKPQSIQESTGS
IIEVLSKIDSEGGVSANHTSRATSTATSGFAGAIGQKLPPFSYAYTELEAIMYALGVG
ASIKDPKDLKFIYEGSSDFSCLPTFGVIIGQKSMMGGGLAEIPGLSINFAKVLHGEQY
LELYKPLPRAGKLKCEAVVADVLDKGSGVVIIMDVYSYSEKELICHNQFSLFLVGSGG
FGGKRTSDKVKVAVAIPNRPPDAVLTDTTSLNQAALYRLSGDWNPLHIDPNFASLAGF
DKPILHGLCTFGFSARRVLQQFADNDVSRFKAVKARFAKPVYPGQTLQTEMWKEGNRI
HFQTKVQETGDIVISNAYVDLAPTSGTSAKTPSEGGKLQSTFVFEEIGRRLKDIGPEV
VKKVNAVFEWHITKGGNIGAKWTIDLKSGSGKVYQGPAKGAADTTIILSDEDFMEVVL
GKLDPQKAFFSGRLKARGNIMLSQKLQMILKDYAKL"
BASE COUNT 722 a 466 c 616 g 691 t
ORIGIN
1 attcgccgcc tcctcctgtc ccgcagtcgg cgtccagcgg ctctgcttgt tcgtgtgtgt
61 gtcgttgcag gccttattca tgggctcacc gctgaggttc gacgggcggg tggtactggt
121 caccggcgcg ggggcagtga atgatttggg aggggacttc aaaggagttg gtaaaggctc
181 cttagctgct gataaggttg ttgaagaaat aagaaggaga ggtggaaaag cagtggccaa
241 ctatgattca gtggaagaag gagagaaggt tgtgaagaca gccctggatg cttttggaag
301 aatagatgtt gtggtcaaca atgctggaat tctgagggat cattcctttg ctaggataag
361 tgatgaagac tgggatataa tccacagagt tcatttgcgg ggttcattcc aagtgacacg
421 ggcagcatgg gaacacatga agaaacagaa gtatggaagg attattatga cttcatcagc
481 ttcaggaata tatggcaact ttggccaggc caattatagt gctgcaaagt tgggtcttct
541 gggccttgca aattctcttg caattgaagg caggaaaagc aacattcatt gtaacaccat
601 tgctcctaat gcgggatcac ggatgactca gacagttatg cctgaagatc ttgtggaagc
661 cctgaagcca gagtatgtgg cacctcttgt cctttggctt tgtcacgaga gttgtgagga
721 gaatggtggc ttgtttgagg ttggagcagg atggattgga aaattacgct gggagcggac
781 tcttggagct attgtaagac aaaagaatca cccaatgact cctgaggcag tcaaggctaa
841 ctggaagaag atctgtgact ttgagaatgc cagcaagcct cagagtatcc aagaatcaac
901 tggcagtata attgaagttc tgagtaaaat agattcagaa ggaggagttt cagcaaatca
961 tactagtcgt gcaacgtcta cagcaacatc aggatttgct ggagctattg gccagaaact
1021 ccctccattt tcttatgctt atacggagct ggaagctatt atgtatgccc ttggagtggg
1081 agcgtcaatc aaggatccaa aagatttgaa atttatttat gaaggaagtt ctgatttctc
1141 ctgtttgccc accttcggag ttatcatagg tcagaaatct atgatgggtg gaggattagc
1201 agaaattcct ggactttcaa tcaactttgc aaaggttctt catggagagc agtacttaga
1261 gttatataaa ccacttccca gagcaggaaa attaaaatgt gaagcagttg ttgctgatgt
1321 cctagataaa ggatccggtg tagtgattat tatggatgtc tattcttatt ctgagaagga
1381 acttatatgc cacaatcagt tctctctctt tcttgttggc tctggaggct ttggtggaaa
1441 acggacatca gacaaagtca aggtagctgt agccatacct aatagacctc ctgatgctgt
1501 acttacagat accacctctc ttaatcaggc tgctttgtac cgcctcagtg gagactggaa
1561 tcccttacac attgatccta actttgctag tctagcaggt tttgacaagc ccatattaca
1621 tggattatgt acatttggat tttctgccag gcgtgtgtta cagcagtttg cagataatga
1681 tgtgtcaaga ttcaaggcag ttaaggctcg ttttgcaaaa ccagtatatc caggacaaac
1741 tctacaaact gagatgtgga aggaaggaaa cagaattcat tttcaaacca aggtccaaga
1801 aactggagac attgtcattt caaatgcata tgtggatctt gcaccaacat ctggtacttc
1861 agctaagaca ccctctgagg gcgggaagct tcagagtacc tttgtatttg aggaaatagg
1921 acgccgccta aaggatattg ggcctgaggt ggtgaagaaa gtaaatgctg tatttgagtg
1981 gcatataacc aaaggcggaa atattggggc taagtggact attgacctga aaagtggttc
2041 tggaaaagtg taccaaggcc ctgcaaaagg tgctgctgat acaacaatca tactttcaga
2101 tgaagatttc atggaggtgg tcctgggcaa gcttgaccct cagaaggcat tctttagtgg
2161 caggctgaag gccagaggga acatcatgct gagccagaaa cttcagatga ttcttaaaga
2221 ctacgccaag ctctgaaggg cacactacac tattaataaa aatggaatca ttaaatactc
2281 tcttcaccca aatatgcttg attattctgc aaaagtgatt agaactaaga tgcaggggaa
2341 attgcttaac attttcagat atcagataac tgcagatttt cattttctac taatttttca
2401 tgtatcatta tttttacaag gaactatata taagctagca cataattatc cttctgttct
2461 tagatctgta tcttcataat aaaaaaattt tgccc
//