LOCUS AK301176 3382 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ59288 partial cds, highly similar to
Roundabout homolog 2 precursor.
ACCESSION AK301176
VERSION AK301176.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3382)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3382
/clone="SPLEN2023779"
/clone_lib="SPLEN2"
/db_xref="H-InvDB:HIT000495800"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="spleen"
CDS 173..>3382
/codon_start=1
/note="highly similar to Roundabout homolog 2 precursor"
/protein_id="BAG62763.1"
/transl_table=1
/translation="MARRHERVTRRMWTWAPGLLMMTVVFWGHQGNGQGQGSRLRQED
FPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLP
SGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVV
AAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYT
CVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKK
DDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVRKMEASATLTVRARPVAPP
QFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSP
TGDLTITNIQRSDAGYYVCQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLA
VDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTC
VATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGT
PGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLS
DPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQP
QFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQ
GMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIW
CLGNETRFHINKTVDAAIRSAIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRR
NEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYA
VTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGD
VLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHE
LAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNGGKGG"
BASE COUNT 968 a 785 c 873 g 756 t
ORIGIN
1 agacagagag tgggagaaac cggggagcag cgggagcagc aggtccgggg ggagctgttc
61 cgctgcgctg ccctcgttat tcacacggac gctgcggagc ttcccagggc tgcttccctg
121 tccccctggg tggaggctgc cgtctaaacc tgactccaga gtttaagatg caatggccag
181 aagacatgaa cgtgtcacta gaaggatgtg gacatgggct ccgggactgt tgatgatgac
241 tgtggtgttt tggggtcatc aggggaatgg acaaggccaa ggatcgcgtc ttcgccagga
301 ggactttccc ccgcggattg tggagcatcc ttccgatgtc atcgtctcta agggcgagcc
361 cacgactctg aactgcaagg cggagggccg gccaacgccc accattgagt ggtacaaaga
421 tggggagcga gtggagactg acaaggacga tccccggtcc cacaggatgc ttctgcccag
481 cggatcctta ttcttcttgc gcatcgtgca cgggcgcagg agtaaacctg atgaaggaag
541 ctacgtttgt gttgcgagga actatcttgg tgaagcagtg agtcgaaatg cgtctctgga
601 agtggcattg ttacgagatg acttccgaca aaaccccaca gatgttgtag tggcagctgg
661 agagcctgca atcctggagt gccagcctcc ccggggacac ccagaaccca ccatctactg
721 gaaaaaagac aaagttcgaa ttgatgacaa ggaagaaaga ataagtatcc gtggtggaaa
781 actgatgatc tccaatacca ggaaaagtga tgcagggatg tatacttgtg ttggtaccaa
841 tatggtggga gaaagggaca gtgacccagc agagctgact gtctttgaac gacccacatt
901 tctcaggagg ccaattaacc aggtggtact ggaggaagaa gctgtagaat ttcgttgtca
961 agtccaagga gatcctcaac caactgtgag gtggaaaaag gatgatgcag acttgccaag
1021 aggaaggtat gacatcaaag acgattacac actaaggatt aaaaagacca tgagtacaga
1081 tgaaggcacc tatatgtgta ttgctgagaa tcgggttaga aaaatggaag cctctgctac
1141 actcaccgtc cgagctcgcc ctgttgctcc cccacagttt gtggttcggc cgagagatca
1201 gattgttgct caaggtcgaa cagtgacatt tccctgtgaa actaaaggaa acccacagcc
1261 agctgttttt tggcagaaag aaggcagcca gaacctactt ttcccaaacc aaccccagca
1321 gcccaacagt agatgctcag tgtcaccaac tggagacctc acaatcacca acattcaacg
1381 ttccgacgcg ggttactacg tctgccaggc tttaactgtg gcaggaagca ttttagcaaa
1441 agctcaactg gaggttactg atgttttgac agatagacct ccacctataa ttctacaagg
1501 cccagccaac caaacgctgg cagtggatgg tacagcgtta ctgaaatgta aagccactgg
1561 tgatcctctt cctgtaatta gctggttaaa ggagggattt acttttccgg gtagagatcc
1621 aagagcaaca attcaagagc aaggcacact gcagattaag aatttacgga tttctgatac
1681 tggcacttat acttgtgtgg ctacaagttc aagtggagag acttcctgga gtgcagtgct
1741 ggatgtgaca gagtctggag caacaatcag taaaaactat gatttaagtg acctgccagg
1801 gccaccatcc aaaccgcagg tcactgatgt tactaagaac agtgtcacct tgtcctggca
1861 gccaggtacc cctggaaccc ttccagcaag tgcatatatc attgaggctt tcagccaatc
1921 agtgagcaac agctggcaga ccgtggcaaa ccatgtaaag accaccctct atactgtaag
1981 aggactgcgg cccaatacaa tctacttatt catggtcaga gcgatcaacc cccaaggtct
2041 cagtgaccca agtcccatgt cagatcctgt gcgcacacaa gatatcagcc caccagcaca
2101 aggagtggac cacaggcaag tgcagaaaga gctaggagat gtccttgtcc gtcttcataa
2161 tccagttgtg ctgactccca ccacggttca ggtcacatgg acggttgatc gccaacccca
2221 gtttatccaa ggctaccgag tgatgtatcg tcagacttca ggtctgcagg cgacatcttc
2281 gtggcagaat ttagatgcca aagtcccgac tgaacgaagt gctgtcttag tcaacctgaa
2341 aaagggggtg acttatgaaa ttaaagtacg gccatatttt aatgagttcc aaggaatgga
2401 tagtgaatct aaaacggttc gtactactga agaagcccca agtgccccac cacagtctgt
2461 cactgtactg acagttggaa gctacaatag cacaagtatt agtgtttcct gggatcctcc
2521 tcctccagat caccagaatg gaattatcca agaatacaag atctggtgtc taggaaatga
2581 aacgcgattc catatcaaca aaactgtgga tgcagccatt cggtccgcaa taattggtgg
2641 attattccca ggtattcaat accgggtaga ggttgcagct agtaccagtg caggggttgg
2701 agtaaagagt gagccacagc caataataat cgggagacgc aatgaagttg tcattactga
2761 aaacaataac agcataactg agcaaatcac tgatgtggtg aagcaaccag cctttatagc
2821 tggtattggt ggtgcctgct gggtaattct gatgggtttt agcatatggt tgtattggcg
2881 aagaaagaag aggaagggac tcagtaatta tgctgttacg tttcaaagag gagatggagg
2941 actaatgagc aatggaagcc gtccaggtct tctcaatgct ggtgatccca gctatccatg
3001 gcttgctgat tcttggccag ccacgagctt gccagtaaat aatagcaaca gtggcccaaa
3061 tgagattgga aattttggcc gtggagatgt gctgccacca gttccaggcc aaggggataa
3121 aacagcaacg atgctctcag atggagccat ttatagtagc attgacttca ctaccaaaac
3181 cagttacaac agttccagcc aaataacaca ggctacccca tatgccacga cacagatctt
3241 gcattccaac agcatacatg aattggctgt cgatctgcct gatccacaat ggaaaagctc
3301 aattcagcaa aaaacagatc tgatgggatt tggttattct ctacctgatc agaacaaagg
3361 taacaatggt gggaaaggtg ga
//