LOCUS AK300974 1776 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ58694 complete cds, highly similar to
NADP-dependent malic enzyme, mitochondrial precursor (EC 1.1.1.40).
ACCESSION AK300974
VERSION AK300974.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1776)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1776
/clone="SMINT2015489"
/clone_lib="SMINT2"
/db_xref="H-InvDB:HIT000495598"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="small intestine"
CDS 328..1356
/codon_start=1
/note="highly similar to NADP-dependent malic enzyme,
mitochondrial precursor (EC 1.1.1.40)"
/protein_id="BAH13386.1"
/transl_table=1
/translation="MGAALGTGTRLAPWPGRACGALPRWTPTAPAQGCHSKPGPARPV
PLKKRGYDVTRNPHLNKGMAFTLEERLQLGIHGLIPPCFLSQDVQLLRIMRYYERQQS
DLDKYIILMTLQDRNEKLFYRVLTSDVEKFMPIVYTPTVGLACQHYGLTFRRPRGLFI
TIHDKGHLATMLNSWPEDNIKAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGV
NPQQCLPVLLDVGTNNEELLRDPLYIGLKHQRVHGKAYDDLLDEFMQAVTDKFGINCL
IQFEDFANANAFRLLNKYRNKYCMFNDDIQGTTCHTFSCPWHLWAQVMFQHIPGPMGF
SEWLPRNS"
BASE COUNT 412 a 522 c 479 g 363 t
ORIGIN
1 aaacgcaaat ccccctctgt gcatttagtc ttgccaagat ggagagtctc aggcgggcat
61 tctgagctgc ttcttgtcac tggggaaaac cacgtgcacg cagtgctgag gcgccccacg
121 cgcgtcctca ggggaagcca ggcactgaga acaaaggttg tgggaccgct ctttgaggaa
181 ctcaccagct ccgcgagctc gcggccgcca gcgcggtcca cccattggcg ggagaggact
241 cacagaccgc agctcctcac cccgcctgct ccgcagcggg accgcgtaga cctcagctcc
301 ccgagcagcg caggtctgcc aaggaccatg ggtgccgcgc tggggacagg cacgcggctg
361 gctccctggc cgggccgggc ctgcggcgcc ctcccgcgct ggacacccac cgcgcccgcc
421 caaggctgcc actccaagcc tggcccggcg cgccctgtgc ccctgaagaa gcgcggatac
481 gatgtcacca ggaaccctca tctcaacaag gggatggcct ttacccttga agaaaggctg
541 cagcttggaa tccacggcct aatcccgccc tgctttctga gccaggacgt ccagctcctc
601 cgaatcatga gatattacga gcggcagcag agtgacctgg acaagtacat cattctcatg
661 acactccaag accggaacga gaagctcttc taccgagtgc tgacttcgga tgtggagaag
721 ttcatgccaa tcgtgtacac gcctaccgtg gggctggcct gtcagcacta tggcctgact
781 ttccgcaggc cccgtggact gttcatcacc attcatgaca aaggtcatct tgcaacaatg
841 ctgaattctt ggccagaaga caatattaag gccgtggtgg tgactgatgg ggagcgcatc
901 ctgggcctgg gagacctggg ctgctacggc atgggcatcc ctgtgggcaa gctggccctg
961 tacacggcat gcggaggggt gaacccgcag cagtgcctcc ctgtgctgct ggacgtcggc
1021 accaacaatg aggagctgct cagagaccct ctgtacatcg gcctgaaaca ccagcgcgtg
1081 cacgggaagg catacgatga cttgctggat gagttcatgc aggctgtgac agacaagttt
1141 ggaataaatt gcctcatcca atttgaagac ttcgccaatg ccaatgcctt ccgcctgctc
1201 aacaaatacc gtaacaagta ctgcatgttc aatgatgaca tccaaggaac aacctgccac
1261 actttcagct gcccatggca tttatgggca caagtcatgt tccagcacat ccctggaccc
1321 atgggcttct cagagtggtt acctcgaaac agctaagtag cttcctttcc agttttaaac
1381 ccctagtgca tagcccaaga gcaccccaaa aatgcaggtt aatgatggag ataactttat
1441 ttgccaaatt ttgtaatttg gagcttaaga accactgttt ttcctcttga gttctggcct
1501 gtaccaagtg cagaaatagc ttcccaaagg aaaaaaatgg gcaacagtac caaatgctgc
1561 tgagaggtca agaaagaaga aggctgagaa ctgattactg aatttggcca cctggaggta
1621 cttggggacc ctgacaagag gcacagcctc cgttgctgtg gcagggatct tggctgctct
1681 gcgaatcacc aacaacaagc tttccaatca cgtgtttgtt ttccaaggtg caggcgaggc
1741 agctatgggc attgcccacc tccttgtcat ggccct
//