LOCUS AK300965 1968 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ57609 complete cds, highly similar to
Villin-1.
ACCESSION AK300965
VERSION AK300965.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1968)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1968
/clone="SMINT2014651"
/clone_lib="SMINT2"
/db_xref="H-InvDB:HIT000495589"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="small intestine"
CDS 230..1780
/codon_start=1
/note="highly similar to Villin-1"
/protein_id="BAG62590.1"
/transl_table=1
/translation="MSHALNFIKAKQYPPSTQVEVQNDGAESAVFQQLFQKWTASNRT
SGLGKTHTVGSVAKVEQVKFDATSMHVKPQVAAQQKMVDDGSGEVQVWRIENLELVPV
DSKWLGHFYGGDCYLLLYTYLIGEKQHYLLYVWQGSQASQDEITASAYQAVILDQKYN
GEPVQIRVPMGKEPPHLMSIFKGRMVVYQGGTSRTNNLETGPSTRLFQVQGTGANNTK
AFEVPARANFLNSNDVFVLKTQSCCYLWCGKGCSGDEREMAKMVADTISRTEKQVVVE
GQEPANFWMALGGKAPYANTKRLQEENLVITPRLFECSNKTGRFLATEIPDFNQDDLE
EDDVFLLDVWDQVFFWIGKHANEEEKKAAATTAQEYLKTHPSGRDPETPIIVVKQGHE
PPTFTGWFLAWDPFKWSNTKSYEDLKAELGNSRDWSQITAEVTSPKVDVFNANSNLSS
GPLPIFPLEQLVNKPVEELPEGVDPSRKEEHLSIEDFTQAFGMTPAAFSALPRWKQQN
LKKEKGLF"
BASE COUNT 503 a 527 c 546 g 392 t
ORIGIN
1 acaattccct gagatctccc aggtggcagc tgcctcccca agacaggctg cactcaaact
61 gtaccatgtg tctgactccg aggggaatct ggtggtgagg gaagtcgcca cacggccact
121 gacacaggac ctgctcagtc acgaggactg ttacatcctg gaccaggggg gcctgaagat
181 ctacgtgtgg aaagggaaga aagccaatga gcaggagaag aagggagcca tgagccatgc
241 gctgaacttc atcaaagcca agcagtaccc accaagcaca caggtggagg tgcagaatga
301 tggggctgag tcggccgtct ttcagcagct cttccagaag tggacagcgt ccaaccggac
361 ctcaggccta ggcaaaaccc acactgtggg ctccgtggcc aaagtggaac aggtgaagtt
421 cgatgccaca tccatgcatg tcaagcctca ggtggctgcc cagcagaaga tggtagatga
481 tgggagtggg gaagtgcagg tgtggcgcat tgagaaccta gagctggtac ctgtggattc
541 caagtggcta ggccacttct atgggggcga ctgctacctg ctgctctaca cctacctcat
601 cggcgagaag cagcattacc tgctctacgt ttggcagggc agccaggcca gccaagatga
661 aattacagca tcagcttatc aagccgtcat cctggaccag aagtacaatg gtgaaccagt
721 ccagatccgg gtcccaatgg gcaaggagcc acctcatctt atgtccatct tcaagggacg
781 catggtggtc taccagggag gcacctcccg aactaacaac ttggagaccg ggccctccac
841 acggctgttc caggtccagg gaactggcgc caacaacacc aaggcctttg aggtcccagc
901 gcgggccaat ttcctcaatt ccaatgatgt ctttgtcctc aagacccagt cttgctgcta
961 tctatggtgt gggaagggtt gtagcgggga cgagcgggag atggccaaga tggttgctga
1021 caccatctcc cggacggaga agcaagtggt ggtggaaggg caggagccag ccaacttctg
1081 gatggccctg ggtgggaagg ccccctatgc caacaccaag agactacagg aagaaaacct
1141 ggtcatcacc ccccggctct ttgagtgttc caacaagact gggcgcttcc tggccacaga
1201 gatccctgac ttcaatcagg atgacttgga agaggatgat gtgttcctac tagacgtctg
1261 ggaccaggtc ttcttctgga ttgggaaaca tgccaacgag gaggagaaga aggccgcagc
1321 aaccactgca caggaatacc tcaagaccca tcccagcggg cgtgaccctg agacccccat
1381 cattgtggtg aagcagggac acgagccccc caccttcaca ggctggttcc tggcttggga
1441 tcccttcaag tggagtaaca ccaaatccta tgaggacctg aaggcggagc ttggcaactc
1501 tagggactgg agccagatca ctgctgaggt cacaagcccc aaagtggacg tgttcaatgc
1561 taacagcaac ctcagttctg ggcctctgcc catcttcccc ctggagcagc tagtgaacaa
1621 gcctgtagag gagctccccg agggtgtgga ccccagcagg aaggaggaac acctgtccat
1681 tgaagatttc actcaggcct ttgggatgac tccagctgcc ttctctgctc tgcctcgatg
1741 gaagcaacaa aacctcaaga aagaaaaagg actattttga gaagagtagc tgtggttgta
1801 aagcagtacc ctaccctgat tgtagggtct cattttctca ccgatattag tcctacacca
1861 attgaagtga aattttgcag atgtgcctat gagcacaaac ttctgtggca aatgccagtt
1921 ttgtttaata atgtacctat tccttcagaa agatgatacc ccaaaagg
//