LOCUS AK300828 1446 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ58461 complete cds, highly similar to Serpin
B7.
ACCESSION AK300828
VERSION AK300828.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1446)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1446
/cell_line="SK-N-SH"
/cell_type="neuroblastoma"
/clone="SKNSH2009756"
/clone_lib="SKNSH2"
/db_xref="H-InvDB:HIT000495452"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
CDS 315..1406
/codon_start=1
/note="highly similar to Serpin B7"
/protein_id="BAG62480.1"
/transl_table=1
/translation="MASLAAANAEFCFNLFREMDDNQGNGNVFFSSLSLFAALALVRL
GAQDDSLSQIDKSGLQSQLKRVFSDINASHKDYDLSIVNGLFAEKVYGFHKDYIECAE
KLYDAKVERVDFTNHLEDTRRNINKWVENETHGKIENVIGEGGISSSAVMVLVNAVYF
KGKWQSAFTKSETINCHFKSPKCSGKAVAMMHQERKFNLSVIEDPSMKILELRYNGGI
NMYVLLPENDLSEIENKLTFQNLMEWTNPRRMTSKYVEVFFPQFKIEKNYEMKQYLRA
LGLKDIFDESKADLSGIASGGRLYISRMMHKSYIEVTEEGTEATAATGSNIVEKQLPQ
STLFRADHPFLFVIRKDDIILFSGKVSCP"
BASE COUNT 420 a 298 c 329 g 399 t
ORIGIN
1 aataagcagc tgcctgtgca gagtgcaggc tgcacctttg gacagccttt aaaactgaat
61 tctcagaatt ttagaacaaa tttttgtcta gaaatgctga ctttggttca ttaggtagtg
121 gtaaaacagg ctcccttcga agctctcctt catcaccttc ctaagtgcat gggggaaaat
181 acctagggct caacagtctt gagaagtgtg gaaacatttt ctttgtgagt gagaacagat
241 cacctagaga aaggagacca gattcccatc actgcttctg ggtatcagat gctagcgctg
301 cactccattt tgcaatggcc tcccttgctg cagcaaatgc agagttttgc ttcaacctgt
361 tcagagagat ggatgacaat caaggaaatg gaaatgtgtt cttttcctct ctgagcctct
421 tcgctgccct ggccctggtc cgcttgggcg ctcaagatga ctccctctct cagattgata
481 agtcagggct ccagtctcaa ctgaaaagag ttttttctga tataaatgca tcccacaagg
541 attatgatct cagcattgtg aatgggcttt ttgctgaaaa agtgtatggc tttcataagg
601 actacattga gtgtgccgaa aaattatacg atgccaaagt ggagcgagtt gactttacga
661 atcatttaga agacactaga cgtaatatta ataagtgggt tgaaaatgaa acacatggca
721 aaatcgagaa cgtgattggt gaaggtggca taagctcatc tgctgtaatg gtgctggtga
781 atgctgtgta cttcaaaggc aagtggcaat cagccttcac caagagcgaa accataaatt
841 gccatttcaa atctcccaag tgctctggga aggcagtcgc catgatgcat caggaacgga
901 agttcaattt gtctgttatt gaggacccat caatgaagat tcttgagctc agatacaatg
961 gtggcataaa catgtacgtt ctgctgcctg agaatgacct ctctgaaatt gaaaacaaac
1021 tgacctttca gaatctaatg gaatggacca atccaaggcg aatgacctct aagtatgttg
1081 aggtattttt tcctcagttc aagatagaga agaattatga aatgaaacaa tatttgagag
1141 ccctagggct gaaagatatc tttgatgaat ccaaagcaga tctctctggg attgcttcgg
1201 ggggtcgtct gtatatatca aggatgatgc acaaatctta catagaggtc actgaggagg
1261 gcaccgaggc tactgctgcc acaggaagta atattgtaga aaagcaactc cctcagtcca
1321 cgctgtttag agctgaccac ccattcctat ttgttatcag gaaggatgac atcatcttat
1381 tcagtggcaa agtttcttgc ccttgaaaat ccaattggtt tctgttatag cagtccccac
1441 aacatc
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