LOCUS AK300815 2162 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ50233 complete cds, highly similar to
Serine/threonine-protein phosphatase with EF-hands 1 (EC 3.1.3.16).
ACCESSION AK300815
VERSION AK300815.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2162)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2162
/cell_line="SK-N-SH"
/cell_type="neuroblastoma"
/clone="SKNSH2005280"
/clone_lib="SKNSH2"
/db_xref="H-InvDB:HIT000495439"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
CDS 195..1961
/codon_start=1
/note="highly similar to Serine/threonine-protein
phosphatase with EF-hands 1 (EC 3.1.3.16)"
/protein_id="BAH13353.1"
/transl_table=1
/translation="MLENYTHIHKEELELRNQSLESEQDMRDRWDYVDSIDVPDSYNG
PRLQFPLTCTDIDLLLEAFKEQQILHAHYVLEVLFETKKVLKQMPNFTHIQTSPSKEV
TICGDLHGKLDDLFLIFYKNGLPSERNPYVFNGDFVDRGKNSIEILMILCVSFLVYPN
DLHLNRGNHEDFMMNLRYGFTKEILHKYKLHGKRILQILEEFYAWLPIGTIVDNEILV
IHGGISETTDLNLLHRVERNKMKSVLIPPTETNRDHDTDSKHNKVGVTFNAHGRIKTN
GSPTEHLTEHEWEQIIDILWSDPRGKNGCFPNTCRGGGCYFGPDVTSKILNKYQLKML
IRSHECKPEGYEICHDGKVVTIFSASNYYEEGSNRGAYIKLCSGTTPRFFQYQVTKAT
CFQPLRQRVDTMENSAIKILRERVISRKSDLTRAFQLQDHRKSGKLSVSQWAFCMENI
LGLNLPWRSLSSNLVNIDQNGNVEYMSSFQNIRIEKPVQEAHSTLVETLYRYRSDLEI
IFNAIDTDHSGLISVEEFRAMWKLFSSHYNVHIDDSQVNKLANIMDLNKDGSIDFNEF
LKAFYVVHRYEDLMKPDVTNLG"
BASE COUNT 696 a 434 c 447 g 585 t
ORIGIN
1 attagaatct atgactgaag aggatcggct aagagtggtt cctcgcagct taaagggagg
61 cacttttcac actctgtctt aaaatcagaa gttgaattca tgaacacata tgatttagat
121 agaagtcatg ggatgcagca gttcttcaac gaaaaccagg agatctgaca catttatcca
181 ccttcttttc cttcatgttg gaaaactaca cacatataca taaggaagag ctagaattaa
241 gaaatcagtc tcttgaaagc gaacaggaca tgagggatag atgggattat gtggactcga
301 tagatgtccc agactcctat aatggtcctc ggctacaatt tcctctcact tgtacggata
361 ttgatttact tcttgaggcc ttcaaggaac aacagatact tcatgcccat tatgtcttag
421 aggtgctatt tgaaaccaag aaagtcctga agcaaatgcc gaatttcact cacatacaaa
481 cttctccctc caaagaggta acaatctgtg gtgatttgca tgggaaactg gatgatcttt
541 ttttgatctt ctacaagaat ggtctcccct cagagaggaa cccgtatgtt tttaatggtg
601 actttgtaga tcgaggaaag aattccatag agatcctaat gatcctgtgt gtgagttttc
661 ttgtctaccc caatgacctg cacttgaaca gagggaacca cgaagatttt atgatgaatc
721 tgaggtatgg cttcacgaaa gaaattttgc ataaatataa gctacatgga aaaagaatct
781 tacaaatctt ggaagaattc tatgcctggc tcccaatcgg tacaatcgtt gacaatgaaa
841 tcctggtcat ccatggtggg atatcagaga ccacagactt gaatttactc caccgtgtag
901 agaggaacaa gatgaaatct gtgctgatac caccaacgga aacaaacaga gaccatgaca
961 ctgactcgaa gcacaataaa gtaggtgtga cttttaatgc acatggaaga atcaaaacaa
1021 atggatctcc tactgaacac ttaacagagc atgaatggga acagattatt gatattctgt
1081 ggagtgatcc cagaggcaaa aatggctgtt ttccaaatac gtgccgagga gggggctgct
1141 attttggacc agatgttact tccaagattc ttaataaata ccagttgaag atgctcatca
1201 ggtctcatga atgtaagccc gaagggtatg aaatctgtca tgatgggaag gtggtgacta
1261 tattttctgc ttctaattat tatgaagaag gcagcaatcg aggagcttac atcaaactat
1321 gttctggtac aactcctcga tttttccagt accaagtaac taaagcaacg tgctttcagc
1381 ctcttcgcca aagagtggat actatggaaa acagcgccat caagatatta agagagagag
1441 tgatttcacg aaaaagtgac cttactcgtg ctttccaact tcaagaccac agaaaatcag
1501 gaaaactttc tgtgagccag tgggcttttt gcatggagaa cattttgggg ctgaacttac
1561 catggagatc cctcagttcg aatctggtaa acatagacca aaatggaaac gttgaataca
1621 tgtccagctt ccagaatatc cgcattgaaa aacctgtaca agaggctcat tctactctag
1681 ttgaaactct gtacagatac agatctgacc tggaaatcat atttaatgcc attgacactg
1741 atcactcagg cctgatctcc gtggaagaat ttcgtgccat gtggaaactt tttagttctc
1801 actacaatgt tcacattgat gattcccaag tcaataagct tgccaacata atggacttga
1861 acaaagatgg aagcattgac tttaatgagt ttttaaaggc tttctatgta gtgcatagat
1921 atgaagactt gatgaaacct gatgtcacca accttggcta aacacaaatg agagcttccc
1981 tcaggctccc tgaaacagct aggcccaaat cacaagtaca gtcctttcca acacccctga
2041 aattcatagt cagtagcaga gaaaagcaga tcccaattca tcccacaaac agatgcatag
2101 tatgggtttt ggaagtccct agcaagctgt tattggtaag attaggttaa atgtcagtaa
2161 ta
//